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Regulatory domain of human heat shock transcription Factor-2 is not regulated by hemin or heat shock

Journal of Cellular Biochemistry, 1999
Heat shock transcription factor 2 (HSF-2) activates transcription of heat shock proteins in response to hemin in the human erythroleukemia cell line, K562. To understand the regulation of HSF-2 activation, a series of deletion mutants of HSF-2 fused to the GAL-4 DNA binding domain were generated.
Z, Zhu, N F, Mivechi
openaire   +2 more sources

Heat Shock Transcription Factors

2022
Klaus-Dieter Scharf, L. Nover
openaire   +1 more source

Bending of promoter DNA on binding of heat shock transcription factor

Nature, 1986
The Drosophila heat-shock transcription factor (HSTF) has been shown to bind to three domains of the heat shock protein 70 gene (hsp 70) control region. The most critical of these for transcriptional activation appears to be the one closest to the TATA-homology region.
D J, Shuey, C S, Parker
openaire   +2 more sources

Expression and Purification of Human Heat-Shock Transcription Factor 1

Protein Expression and Purification, 1997
Heat-shock factor 1 (HSF1) is a 57-kDa cytoplasmic protein which binds to the promoters of heat-shock genes and activates transcription during heat shock. We describe here the expression and purification of the 529 amino acids form of human HSF1. We designed a new and complete purification protocol involving ammonium sulfate precipitation, heparin ...
Soncin, Fabrice   +2 more
openaire   +3 more sources

In vitro activation of heat shock transcription factor by 4-hydroxynonenal

Chemico-Biological Interactions, 1992
In the activation of eukaryotic heat shock genes, the acquisition of a binding ability to specific DNA sequence by a transcriptional activator, heat shock factor (HSF), is believed to be a crucial step. The induction of this new DNA binding activity of HSF is also obtained in a cell-free system (in vitro activation) by hyperthermia or at physiological ...
F, Cajone, M, Crescente
openaire   +2 more sources

Increased temperature, not cardiac load, activates heat shock transcription factor 1 and heat shock protein 72 expression in the heart

American Journal of Physiology - Regulatory Integrative and Comparative Physiology, 2007
Scott Powers   +2 more
exaly  

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