Results 21 to 30 of about 1,211,483 (357)

A simple way to increase recovery of the expressed VH and VL genes in single-sorted human B cells

BioTechniques, 2019
Cloning VH and VL genes from individual antigen-specific B cells is an attractive approach for producing monoclonal antibodies of the desired specificity.
Sergey V Guselnikov, Tatyana N Belovezhets, Sergey V Kulemzin, Andrey A Gorchakov, Alexander V Taranin   +4 more
doaj   +1 more source

Differences in human IgG1 and IgG4 S228P monoclonal antibodies viscosity and self-interactions: Experimental assessment and computational predictions of domain interactions

mAbs, 2021
Human/humanized IgG4 antibodies have reduced effector function relative to IgG1 antibodies, which is desirable for certain therapeutic purposes. However, the developability and biophysical properties for IgG4 antibodies are not well understood. This work
Pin-Kuang Lai, Gaurav Ghag, Yao Yu, Veronica Juan, Laurence Fayadat-Dilman, Bernhardt L. Trout   +5 more
doaj   +1 more source

Light and heavy chain deposition disease with focal amyloid deposition diagnosed with mass spectrometry: a case report

BMC Nephrology, 2023
Background Light and heavy chain deposition disease (LHCDD) is a rare condition characterised by the deposition of immunoglobulin components in the kidneys.
Yuki Shimamoto, Naoki Takahashi, Nagaaki Katoh, Yuki Matsui, Yusuke Mochizuki, Masanori Ito, Masahide Yazaki, Fuyuki Kametani, Kenji Kasuno, Yoshiki Sekijima, Hironobu Naiki, Masayuki Iwano   +11 more
doaj   +1 more source

A role for Tctex-1 (DYNLT1) in controlling primary cilium length [PDF]

, 2011
The microtubule motor complex cytoplasmic dynein is known to be involved in multiple processes including endomembrane organization and trafficking, mitosis, and microtubule organization.
MacCarthy-Morrogh, LJ, Miss Nicola, Smyllie, Palmer, KJ, Stephens, DJ   +3 more
core   +1 more source

Vibration of a Flexible Hanging Chain with a Mass on the End in a Nonstationary Regime of the Motion Mechanism in Hoisting Machines [PDF]

Journal of Applied and Computational Mechanics
The article is focused on the vibrations of a heavy chain with a mass on the end when the hoisting machines are in a nonstationary regime of motion, assuming that motor or brake torque is a linear function of the speed of the motion mechanism.
Vassil Zlatanov, Svetoslav Nikolov
doaj   +1 more source

Analysis of monoclonal immunoglobulins from bone marrow plasma cells using immunopurification and LC-MS

Journal of Mass Spectrometry and Advances in the Clinical Lab, 2023
Introduction: Clonal plasma cells secrete immunoglobulins, each with the exact same amino acid sequence, that are referred to as monoclonal immunoglobulins.
David R. Barnidge, Angela Dispenzieri, Dragan Jevremovic, David L. Murray   +3 more
doaj   +1 more source

Layer guided-acoustic plate mode biosensors for monitoring MHC-peptide interactions [PDF]

, 2006
The transduction signals from the immobilisation of a class I heavy chain, HLA-A2, on a layer guided acoustic plate mode device, followed by binding of beta(2)-microglobulin and subsequent selective binding of a target peptide are ...
Dodi, I. A., Evans, Carl, McHale, Glen, Newton, Michael, Paston, S. J., Percival, Carl, Rees, Robert, Stanley, Simon   +7 more
core   +1 more source

Acquisition of N-Glycosylation Sites in Immunoglobulin Heavy Chain Genes During Local Expansion in Parotid Salivary Glands of Primary Sjögren Patients

Frontiers in Immunology, 2018
Previous studies revealed high incidence of acquired N-glycosylation sites acquired N-glycosylation sites in RNA transcripts encoding immunoglobulin heavy variable region (IGHV) 3 genes from parotid glands of primary Sjögren’s syndrome (pSS) patients. In
Annie Visser, Marieke E. Doorenspleet, Marieke E. Doorenspleet, Marieke E. Doorenspleet, Niek de Vries, Niek de Vries, Fred K. L. Spijkervet, Arjan Vissink, Richard J. Bende, Hendrika Bootsma, Frans G. M. Kroese, Nicolaas A. Bos   +11 more
doaj   +1 more source

Gamma-heavy chain disease

Klinicka Onkologie, 2020
Gamma-heavy chain disease is a rare disease, described so far in approximately 150 cases. The aim of this work was laboratory dia-gnostics of immunoglobulin heavy chain disease.A 60-year-old patient was referred to the University Hospital in Ostrava for suspected marginal zone lymphoma from gastric bio-psy.
Tomas Jelinek, Roman Hájek, David Zeman, Pavlína Kušnierová   +3 more
openaire   +2 more sources

Crystal Structure and Characterization of Human Heavy-Chain Only Antibodies Reveals a Novel, Stable Dimeric Structure Similar to Monoclonal Antibodies

Antibodies, 2020
We report the novel crystal structure and characterization of symmetrical, homodimeric humanized heavy-chain-only antibodies or dimers (HC2s). HC2s were found to be significantly coexpressed and secreted along with mAbs from transient CHO HC/LC ...
Carl Mieczkowski, Soheila Bahmanjah, Yao Yu, Jeanne Baker, Gopalan Raghunathan, Daniela Tomazela, Mark Hsieh, Mark McCoy, Corey Strickland, Laurence Fayadat-Dilman   +9 more
doaj   +1 more source