Results 31 to 40 of about 1,244,465 (348)

Chimerization of antibodies by isolation of rearranged genomic variable regions by the polymerase chain reaction [PDF]

, 1991
We describe a new method for amplification, by polymerase chain reaction (PCR), of rearranged segments encoding the variable part of light and heavy chains of an antibody (Ab) from the chromosomal DNA of hybridoma cells for the chimerization ofAbs.
Kaluzab, Brigitte, Schwirzke, Marina, Weidle, Ulrich H., Weiss, Elisabeth H., Weissenhorn, Winfried   +4 more
core   +1 more source

AL amyloidosis with non-amyloid forming monoclonal immunoglobulin deposition; a case mimicking AHL amyloidosis

BMC Nephrology, 2018
Background Immunoglobulin heavy-and-light-chain amyloidosis (AHL amyloidosis) is a newly established disease entity where both the immunoglobulin heavy-chain and light-chain compose amyloid fibrils. The immunoglobulins responsible for the amyloid fibrils
Shun Manabe, Chihiro Iwasaki, Michiyasu Hatano, Fuyuki Kametani, Masahide Yazaki, Kosaku Nitta, Michio Nagata   +6 more
doaj   +1 more source

Data to understand the biotransfer of heavy metals along the soil-plant-edible insect-human food chain in Africa

Data in Brief, 2023
Data on the biotransfer of heavy metals along the soil-plant-edible insect-human food chain collected along a 60km pollution gradient is presented here.
Susan Mwelwa, Donald Chungu, Frank Tailoka, Dennis Beesigamukama, Chrysantus M. Tanga   +4 more
doaj   +1 more source

AIRR-C IG Reference Sets: curated sets of immunoglobulin heavy and light chain germline genes

Frontiers in Immunology
IntroductionAnalysis of an individual’s immunoglobulin (IG) gene repertoire requires the use of high-quality germline gene reference sets. When sets only contain alleles supported by strong evidence, AIRR sequencing (AIRR-seq) data analysis is more ...
Andrew M. Collins, Mats Ohlin, Martin Corcoran, James M. Heather, James M. Heather, Duncan Ralph, Mansun Law, Jesus Martínez-Barnetche, Jian Ye, Eve Richardson, William S. Gibson, Oscar L. Rodriguez, Ayelet Peres, Gur Yaari, Corey T. Watson, William D. Lees, William D. Lees   +16 more
doaj   +1 more source

Immunoglobulin heavy chain exclusion in the shark. [PDF]

PLoS Biology, 2008
The adaptive immune system depends on specific antigen receptors, immunoglobulins (Ig) in B lymphocytes and T cell receptors (TCR) in T lymphocytes.
Karolina Malecek, Victor Lee, Wendy Feng, Jing Li Huang, Martin F Flajnik, Yuko Ohta, Ellen Hsu   +6 more
doaj   +1 more source

Cloning and expression of a human kinesin heavy chain gene: interaction of the COOH-terminal domain with cytoplasmic microtubules in transfected CV-1 cells. [PDF]

, 1992
To understand the interactions between the microtubule-based motor protein kinesin and intracellular components, we have expressed the kinesin heavy chain and its different domains in CV-1 monkey kidney epithelial cells and examined their distributions ...
Bernstein, HD, Hom-Booher, N, McCaffrey, G, Navone, F, Niclas, J, Sparks, L, Vale, RD   +6 more
core  

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Diversity in the HLA-I Recognition of HLA-F Monoclonal Antibodies: HLA-F or HLA-Ib Monospecific, HLA-E or HLA-G Bispecific Antibodies with or without HLA-Ia Reactivity

Antibodies
Previous investigators have used various anti-HLA-F monoclonal antibodies (mAbs) to demonstrate that the tissue distribution of HLA-F is highly restricted. Notably, these mAbs differed in their immunodiagnostic capabilities.
Mepur H. Ravindranath, Narendranath M. Ravindranath, Carly J. Amato-Menker, Fatiha El Hilali, Edward J. Filippone   +4 more
doaj   +1 more source

Partially functional outer arm dynein in a novel Chlamydomonas mutant expressing a truncated γ heavy chain [PDF]

, 2008
The outer dynein arm of Chlamydomonas flagella contains three heavy chains (α, β, and γ), each of which exhibits motor activity. How they assemble and cooperate is of considerable interest.
Kamiya Ritsu, King Stephen M., Liu Zhongmei, Nakazawa Yuki, Sakato Miho, Takazaki Hiroko, Yagi Toshiki, Yasunaga Takuo   +7 more
core   +2 more sources

Cell wall target fragment discovery using a low‐cost, minimal fragment library

FEBS Letters, EarlyView.
LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan, Mathew Stanley, Olawale Raimi, Andrew T. Ferenbach, Helge C Dorfmueller, Daan M. F. van Aalten   +5 more
wiley   +1 more source