Sodium Current Measurements in HEK293 Cells
Bio-Protocol, 2013 This protocol is used to measure sodium currents from heterologously transfected cells lines, such as HEK293 cells. Standard whole cell patch clamp technique is used to assess ion channel function. This protocol has been used to study cardiac type Nav1.5
Helena Riuró +5 more
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Antioxidant Efficacy of Esculetin against Tert-Butyl Hydroperoxide-Induced Oxidative Stress in HEK293 Cells [PDF]
Current Issues in Molecular Biology, 2022 Esculetin is an antioxidant and anti-inflammatory compound derived from coumarin. Oxidative stress can cause overproduction of reactive oxygen species (ROS), which can lead to the development of chronic kidney failure.
Woo Kwon Jung +4 more
doaj +2 more sources
M1 muscarinic receptor activation mediates cell death in M1-HEK293 cells. [PDF]
PLoS ONE, 2013 HEK293 cells have been used extensively to generate stable cell lines to study G protein-coupled receptors, such as muscarinic acetylcholine receptors (mAChRs).
E Scott Graham +6 more
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Expression of Large Full-Length PfEMP1 Proteins in HEK293 Cells. [PDF]
Methods Mol Biol, 2022 Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) is a family of proteins expressed on the surface of red blood cells infected by Plasmodium falciparum. PfEMP1 proteins play a vital role in parasite virulence, and thus are important vaccine candidates to prevent severe disease.
Renn JP, Doritchamou JYA, Duffy PE.
europepmc +3 more sources
Scalable Production of AAV Vectors in Orbitally Shaken HEK293 Cells [PDF]
Molecular Therapy: Methods & Clinical Development, 2019 Adeno-associated virus (AAV) vectors are currently among the most commonly applied for in vivo gene therapy approaches. The evaluation of vectors during clinical development requires the production of considerable amounts of highly pure and potent ...
Daniel Blessing +7 more
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MicroRNA and Hemophilia-A Disease: Bioinformatics Prediction and Experimental Analysis [PDF]
Cell Journal, 2021 Objective: Hemophilia-A is a common genetic abnormality resulted from decreased or lack of factor VIII (FVIII) pro-coagulant protein function caused by mutations in the F8 gene.
Halimeh Rezaei +2 more
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Kidney Diseases, 2022 Introduction: Acute kidney injury (AKI) is a major public health problem worldwide. However, there is no definitive therapies to treat established AKI. In this study, we used FG-4592 to induce hypoxia inducible factor (HIF) expression in cells and then ...
Zhao-Ying Ding +7 more
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A Comparative Study on the Efficiency of CRISPR-Cas9 in Human Embryonic Kidney 293 Cells and Peripheral Blood Mononuclear Cells for Disruption in Programmed Cell Death Protein 1 [PDF]
Journal of Genetic Resources, 2022 CRISPR-Cas9 is the most important tool in genome engineering in recent years. The efficiency of this instrument on active and non-active genes is variable.
Seyed Ali Alambeladi +2 more
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HEK293S Cells Have Functional Retinoid Processing Machinery [PDF]
The Journal of General Physiology, 2002 Rhodopsin activation is measured by the early receptor current (ERC), a conformation-associated charge motion, in human embryonic kidney cells (HEK293S) expressing opsins. After rhodopsin bleaching in cells loaded with 11-cis-retinal, ERC signals recover in minutes and recurrently over a period of hours by simple dark adaptation, with no added ...
Brueggemann, Lioubov I. +1 more
openaire +2 more sources
Distinct mechanical behavior of HEK293 cells in adherent and suspended states [PDF]
PeerJ, 2015 The mechanical features of individual animal cells have been regarded as indicators of cell type and state. Previously, we investigated the surface mechanics of cancer and normal stromal cells in adherent and suspended states using atomic force ...
Seyed Mohammad Ali Haghparast +2 more
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