Results 151 to 160 of about 4,870,076 (306)

H4K12 Lactylation Regulates NDUFS7 to Drive Microglia Reverse Electron Transport in Spinal Cord Injury

Advanced Science, EarlyView.
Lactate accumulation after SCI promotes H4K12la lactylation, which upregulates NDUFS7, triggering mitochondrial RET and ROS, enforcing pro‐inflammatory microglial polarization. MM@mPTC, a biomimetic nanotherapeutic, degrades LDHA in activated microglia, reducing H4K12la lactylation and blocking RET, thus curbing ROS, fostering a reparative phenotype ...
Chenglong Hong, Zhichen Jiang, Zhouwei Wu, Chenyu Wu, Jiang Liu, Kaijie Guo, Yihui Liang, Shaobo Xu, Yuchen Jin, Yong Xiao, Lei Guo, Hui Xu, Sunren Sheng, Chenggui Wang   +13 more
wiley   +1 more source

Tissue culture protocol for HEK293 cells v1

Lewis lab tissue culture protocol for HEK293 cells.
Susanne Herbst, Patrick Lewis
openaire   +1 more source

UBIAD1 is accumulated in the juxta-nuclear area inside HEK293, T24 and PC-3 cells.

, 2013
A, Bioinformatics analysis indicates that UBIAD1 is a putative eight transmembrane protein. B, Subcellular localization of UBIAD1 in HEK293, T24 and PC-3 cells are similar.
Pan Jing (447402), Ling Hong (447405), Yuangan Wu (447403), Yanzhi Xia (447404), Dangfeng Wang (447401), Xian Wang (15374), Maorong Chen (45925)   +6 more
core   +1 more source

WFS1 Deficiency Impairs PIAS4‐Associated SUMOylation and Increases Ubiquitin‐Mediated Spermatogenesis‐Related Protein Degradation Leading to Testicular Male Infertility

Advanced Science, EarlyView.
The findings establish a critical role for WFS1 in human male fertility. Mechanistically, WFS1 interacts with PIAS4 to promote the SUMOylation of key spermatogenesis‐associated proteins, which in turn competitively inhibits their K48‐linked ubiquitin‐mediated degradation during spermatogenesis.
Yunchuan Tian, Guicheng Zhao, Xiang Wang, Xinyao Tang, Gan Shen, Tiechao Ruan, Chuan Jiang, Shikun Zhao, Jincheng Zhang, Xinyue Cui, Liangchai Zhuo, Jun Ma, Yihong Yang, Tingting Lin, Ying Shen   +14 more
wiley   +1 more source

Immunofluorescence of transfected HEK293 cells.

, 2015
Double-immunofluorescence of contactin-1-, neurofascin-155- and neurofascin-186-transfected HEK293 cells with a control anti-contactin antibody (A, J), control anti-neurofascin155 (D), control anti-neurofascin186 (G) and sera of patients with MMN (B, E ...
Heidrun H. Krämer (776339), Stephen G. Waxman (504800), Kathrin Doppler (776337), Judy King Man Ng (776340), Sulayman D. Dib-Hajj (504799), Andreas Weishaupt (230628), Luise Appeltshauser (776338), Peter Brophy (776343), Edgar Meinl (776341), Carmen Villmann (776342), Claudia Sommer (230646)   +10 more
core   +1 more source

EphB1 Receptor Blockade Augments Morphine Antinociception via Inhibiting GRK2‐Mediated µ‐Opioid Receptor Internalization

Advanced Science, EarlyView.
Administration of opioids activates ephrinB2‐EphB1 receptor signaling. Activated EphB1 receptors induce GRK2‐mediated phosphorylation of MOR at Ser375, which initiates the internalization of MOR and reduces opioid antinociception at the behavioral level. ABSTRACT Opioids are the primary treatment for severe pain in clinical practice.
Bo Peng, Xiaqing Lin, Hongyu Yao, Cheng Li, Huabao Liao, Yuexin Liu, Boxu Cao, Xue‑Jun Song   +7 more
wiley   +1 more source

The Cytotoxic Effect of Metformin on Cervical Cancer (Hela) Cells in Comparison with Non-Cancerous Kidney Cells

Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum, 2018
Background and Objectives: Studies have shown that antidiabetic drugs can inhibit cancer cells proliferation. The aim of this study was to investigate the cytotoxic effects of metformin on cervical cancer (Hela) cells in comparison with non-cancerous ...
Zahra Gholamhoseyni, Rahim Ahmadi, Yeganeh Hamidi   +2 more
doaj  

Live and Let Live: The Remarkable Story of HEK293 Cells [PDF]

Human Gene Therapy, 2020
openaire   +2 more sources

HSP70 stabilizes TRIM63-containing CBs in HEK293 cells.

, 2017
(A) HEK293 cells were transiently transfected with FLAG-TRIM63 (+) or vector alone (-). The association of TRIM63 with endogenous HSP70 was analyzed with co-immunoprecipitation followed by western blotting (WB).
Takahiro Takekiyo (2293357), Tohru Ichimura (2486053), Masato Taoka (189054), Ikuo Shoji (127861), Naomi Hachiya (3615488), Takamasa Uekita (3615485), Yuki Kawaguchi (1706257)   +6 more
core   +1 more source

Mechanically Regulated Secretion: How Physical Forces Instruct the Secretory Pathway and Remodel the Secretome

Advanced Science, EarlyView.
Mechanical cues, including ECM stiffness, stretch, compression, shear stress, and traction forces, remodel trafficking across the secretory pathway, from the ER and Golgi to endolysosomal compartments and the plasma membrane. The resulting secretome reshapes ECM composition and tissue mechanics, establishing feedback loops that support homeostasis or ...
Domenico Russo, Seetharaman Parashuraman, Maria Luigia Maresca, Angela Francesca Dinoi, Alessia Calcagnì, Riccardo Rizzo   +5 more
wiley   +1 more source