Results 141 to 150 of about 150,776 (304)

Effects of MWCNTs on Kv4.2 trafficking in HEK293 cells.

, 2014
A, an original recording of flow cytometry showing the fluorescence of Flag which represented the population of Kv4.2 in cell membrane. A leftward shift of the trace meant a reduction of fluorescence intensity in membrane and vise versa.
Ji-Min Cao (465416), Qing-Hua Liu (592193), Bo-Wei Wu (592192), Li Zhang (8200), Xiu-Li Cheng (592191), Jie Meng (152665), Hai-Yan Xu (558074), Xiao-Qiu Tan (592190)   +7 more
core   +1 more source

ZDHHC18‐Mediated Palmitoylation of ORF3a Promotes SARS‐CoV‐2 Pathogenesis by Antagonizing TRIM16‐Mediated Ubiquitination and Proteasomal Degradation

Advanced Science, EarlyView.
Palmitoylation by ZDHHC18 blocks ORF3a K27‐linked ubiquitination mediated by TRIM16, thereby preventing its proteasomal degradation and strengthening viral pathogenesis. Targeting palmitoylation through a pharmacological inhibitor (2‐BP), a competitive inhibitory peptide (OPIP), or adenovirus‐mediated knockdown of ZDHHC18 expression presents a ...
Sidi Yang, Kun Li, Lihong Liu, Linsen Zeng, Qifeng Deng, Jiacheng Huang, Xiaoran Dong, Xin Wang, Jianwei Liang, Hongchao Liu, Hong Peng, Yuxin Lin, Xiaolu Xie, Yuzhen Ye, Tiefeng Xu, Zhaohuan Wang, Chun‐Mei Li, Deyin Guo   +17 more
wiley   +1 more source

Close up of a capsule, revealing living HEK293 cells inside.

, 2014
Close up of a capsule, revealing living HEK293 cells inside.
Łukasz Adaszek (599076), Monika Michałowska (599074), Wojciech Łopuszyński (599077), Zbigniew Grądzki (599078), Stanislaw Winiarczyk (599075), Walter H. Günzburg (315731), Brian Salmons (77633)   +6 more
core   +1 more source

Balancing Stealth and Targetability: cCPP‐ and PEG‐Modified Liposomes for the Targeted Delivery of Anti‐HER2 Nanobodies

Advanced Science, EarlyView.
This study addresses the rapid renal clearance of anti‐HER2 nanobodies by developing specialized liposomal carriers. It details the formulation of dual modified liposomes, demonstrating how PEGylation preserves structural integrity and cell‐penetrating peptides enhance cellular interaction.
Giulia Pander, Maria del Pilar Palacios Cisneros, Clara Certa, Megan Stierli, Katharina Beck, Nicolas Färber, Lisa Blank, Fiona Tanner, Eric Mühlberg, Sabrina Wohlfart, Christian Kleist, Jörg Huwyler, Gert Fricker, Walter Mier, Philipp Uhl   +14 more
wiley   +1 more source

Development of HEK293T cell reference materials for β-thalassemia genetic testing using prime editing

Türk Biyokimya Dergisi
β-Thalassemias, caused by mutations in HBB, are hereditary blood disorders that impose a significant global health burden. Detecting these mutations through accurate genetic analysis is essential. This study aimed to create a panel of cell type reference
Ren Baoyan, Lu Jiahao, Tan Weihe, Lin Kangfeng, Xu Jingping, Zheng Yu, Xing Xingan, Zhou Qiaomiao   +7 more
doaj   +1 more source

BACE2 modulates human IAPP protein levels HEK293 cells.

, 2016
(A) RNAseq FPKM values for basal expression of human IAPP, BACE2, BACE1, PCSK1, PCSK2 and APP in HEK293 cells. (B) HEK293 cells co-transfected with, or without, hIAPP plasmid DNA (1μg) and varying concentrations of hBACE2 plasmid DNA, hBACE1 plasmid DNA,
Jonitha Gardner (328549), Helene Baribault (328546), Ping Cao (564151), Li Miao (734548), Trace Tsuruda (2011873), Yang Li (7082), Linda Huang (2141800), Kui Chen (157443), Xiaosu Wu (756219), Jed Wiltzius (2141794), Ryan D. White (1656982), Oliver Homann (2141806), Harvey Yamane (559007), Ingrid C. Rulifson (2141809), Kim Samayoa (2141803), David Kopecky (2141812), Tim Carlson (2141797)   +16 more
core   +1 more source

A Plug‐and‐Play Platform for Customizing Multivalent Degraders and Degrader‐Drug Conjugates

Advanced Science, EarlyView.
Membrane proteins remain challenging targets for conventional TPD approaches. Here, the authors develop UPTAB, a modular platform leveraging ultrahigh‐affinity orthogonal Im/CL protein pairs for lysosomal degradation of membrane proteins. Mono‐targeted (Type‐I), dual‐targeted (Type‐II), and tri‐targeted (Type‐III) UPTABs enable simultaneous degradation
Mengqing Zhao, Yan Deng, Jianjian Han, Yong Xie, Kai Bao, Lixin Ma, Lilong Liu, Wuxiang Mao   +7 more
wiley   +1 more source

A Novel Class of “Super‐Strained” Spiro Heterocycles: Gateway to 1‐Azaspiro[3.3]heptane Derivatives, and Biological Validation

Angewandte Chemie, EarlyView.
A new class of “super‐strained” spiro heterocycles—spirocyclic 1‐azabicyclo[1.1.0]butanes—was synthesized via insertion of cyclobutane‐, oxetane‐, and azetidine‐containing sulfonium reagents into substituted azirines. The stability of this new class of compounds was studied.
Philipp Natho, Annarita Vicenti, Marco Colella, Francesco Pasca, Giuseppe Romanazzi, Mauro Niso, Carmen Abate, Ernesto Mesto, Emanuela Schingaro, Renzo Luisi   +9 more
wiley   +2 more sources

The Cytotoxic Effect of Metformin on Cervical Cancer (Hela) Cells in Comparison with Non-Cancerous Kidney Cells

Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum, 2018
Background and Objectives: Studies have shown that antidiabetic drugs can inhibit cancer cells proliferation. The aim of this study was to investigate the cytotoxic effects of metformin on cervical cancer (Hela) cells in comparison with non-cancerous ...
Zahra Gholamhoseyni, Rahim Ahmadi, Yeganeh Hamidi   +2 more
doaj  

Tissue culture protocol for HEK293 cells v1

Lewis lab tissue culture protocol for HEK293 cells.
Susanne Herbst, Patrick Lewis
openaire   +1 more source