Results 51 to 60 of about 4,870,076 (306)

Production and Purification of Zika Virus NS1 Glycoprotein in HEK293 Cells [PDF]

, 2020
In this chapter, we describe production and purification of the Zika virus NS1 glycoprotein in human embryonic kidney (HEK293T) cells at small, research laboratory scale. The expression of secreted NS1 (sNS1) and the C-terminal β-ladder domain in HEK293T cells were tested in a small-scale transfection before scaling up to a larger-scale transfection ...
Kim, YC, Garcia-Larragoiti, N, Lopez-Camacho, C, Viveros-Sandoval, ME, Reyes-Sandoval, A   +4 more
openaire   +2 more sources

Patulin suppresses α1-adrenergic receptor expression in HEK293 cells [PDF]

Scientific Reports, 2020
AbstractPatulin (PAT) is a common mycotoxin contaminant of apple products linked to impaired metabolic and kidney function. Adenosine monophosphate activated protein kinase (AMPK), abundantly expressed in the kidney, intercedes metabolic changes and renal injury.
Pillay, Yashodani, Nagiah, Savania, Phulukdaree, Alisa, Krishnan, Anand, Chuturgoon, Anil A.   +4 more
openaire   +3 more sources

Generation of Mt BART(+/−)·S13^- HEK293-EBV cells.

, 2023
A) Schematic diagram of BART(+/-)·S13+ EBV bacmids. A CTCF-binding site on the BART miRNA promoter was identified as BARTp CTCF BS. B) Sequences introduced at the points of recombination to introduce site-directed mutations at the S13 locus (EBV 138963 ...
Sora Huh (14504838), Hyosun Cho (267146), Donghyun Seo (1611292), Hideki Tanizawa (3175911), Seong Ho An (14504841), Minhee Lee (2194918), Miyeon Cho (2505967), Kyuhyun Kang (14504844), Hyojeung Kang (209084), Sun Hee Lee (667423), Kyoung-Dong Kim (14504835), Inuk Jung (3602396)   +11 more
core   +1 more source

25-Hydroxyvitamin D3 1-Alpha-Hydroxylase-Dependent Stimulation of Renal Klotho Expression by Spironolactone

Kidney & Blood Pressure Research, 2013
Background: Klotho, a transmembrane protein, protease and hormone mainly expressed in kidney, is required for the suppression of 1,25(OH)2D3-generating 25-hydroxyvitamin D3 1-alpha-hydroxylase (Cyp27b1) by FGF23.
Ioana Alesutan, Martina Feger, Tatsiana Pakladok, Sobuj Mia, Mohamed Siyabeldin E. Ahmed, Jakob Voelkl, Florian Lang   +6 more
doaj   +1 more source

Impact of cell type and epitope tagging on heterologous expression of G protein-coupled receptor: a systematic study on angiotensin type II receptor. [PDF]

PLoS ONE, 2012
Despite heterologous expression of epitope-tagged GPCR is widely adopted for functional characterization, there is lacking of systematic analysis of the impact of expression host and epitope tag on GPCR expression.
Lili Jiang, Gladys M K Teng, Elaine Y M Chan, Shannon W N Au, Helen Wise, Susanna S T Lee, Wing-Tai Cheung   +6 more
doaj   +1 more source

The pharmacology of hSK1 Ca2+-activated K+ channels expressed in mammalian cell lines. [PDF]

, 2000
The pharmacology of hSK1, a small conductance calcium-activated potassium channel, was studied in mammalian cell lines (HEK293 and COS-7). In these cell types, hSK1 forms an apamin-sensitive channel with an IC(50) for apamin of 8 nM in HEK293 cells and ...
Shah, M, Haylett, DG
core  

Immunoblotting of ABCB1/P-gp (A) and MRP7 (B) in HEK293 and HEK293/MRP7 cells and Immunofluorescence detection of MRP7 in HEK293/MRP7 cells (C).

, 2013
Cell lysates were prepared from HEK293/pcDNA3.1, HEK293/ABCB1 and HEK293/MRP7 cells (A), HEK293/MRP7 cells incubated with 5 µM BBA for different time periods (0, 24, 48 and 72 h) (B).
Atish Patel (285161), Dong-Mei Zhang (303527), Zhi-Jie Xiao (459853), Jun-Jiang Chen (285154), Amit K. Tiwari (223070), Wen-Cai Ye (303536), Ying-Jie Li (459854), Kamlesh Sodani (285160), Si-Dong Chen (285163), Dong-Hua Yang (223092), Zhe-Sheng Chen (223107)   +10 more
core   +1 more source

The heterodimeric amino acid transporters (HAT) of the SLC7/SLC3 family: A structure−function relationships and relevance to human pathology

FEBS Letters, EarlyView.
Heterodimeric amino acid transporters consist of SLC7 and SLC3 family proteins arranged in a conserved structural organization. They regulate nutrient transport across cell membranes, supporting essential cellular functions. These transporters also contribute to xenobiotic/drug uptake and distribution.
Mariafrancesca Scalise, Tiziano Mazza, Raffaella Scanga, Lara Console, Michele Galluccio, Cesare Indiveri   +5 more
wiley   +1 more source

Effects of preincubation of HEK293 and HEK293/MRP4 cells with MK571 or Cpd23 at 50 μM on the accumulation of 6-MP.

, 2018
Y-axis represents the ratio of 6-MP concentration compared to only 6-MP treated HEK293 or HEK293/MRP4 cells, respectively. 293+MK571 and 293+Cpd23 stand for HEK293 cells with MK571 or Cpd23-treated, while MRP4+MK571 and MRP4+Cpd23 stand for HEK293/MRP4 ...
Zhenming Liu (448850), Hongwei Jin (391981), Ya Chen (1437550), Liangren Zhang (114208), Xia Yuan (485523), Zhangping Xiao (5855825)   +5 more
core   +1 more source

Leucine‐rich glioma inactivated 1 (LGI1) is a ganglioside‐binding protein

FEBS Letters, EarlyView.
Neuronal hyperexcitability associated with a decrease/absence of the extracellular protein LGI1 has been suggested to be primarily due to the downregulation of Kv1 channel expression. The molecular mechanisms underlying this decrease have not yet been elucidated.
Kévin Debreux, Christian Lévêque, Fodil Azzaz, Marion Sangiardi, Sarosh R Irani, Michael Seagar, Jacques Fantini, Oussama El Far   +7 more
wiley   +1 more source