Results 51 to 60 of about 150,776 (304)
Rabies virus-like particles expressed in HEK293 cells
Vaccine, 2014 Rabies is an infectious viral disease with a mortality rate close to 100%. Currently, there is a need to generate cheaper and more immunogenic vaccines for the effective prevention of rabies, mostly in developing countries. Virus-like particles have been widely used in viral vaccine production due to their high immunogenicity and safety during the ...
Fontana, Diego Sebastian +3 more
openaire +3 more sources
Production and Purification of Zika Virus NS1 Glycoprotein in HEK293 Cells [PDF]
, 2020 In this chapter, we describe production and purification of the Zika virus NS1 glycoprotein in human embryonic kidney (HEK293T) cells at small, research laboratory scale. The expression of secreted NS1 (sNS1) and the C-terminal β-ladder domain in HEK293T cells were tested in a small-scale transfection before scaling up to a larger-scale transfection ...
Kim, YC +4 more
openaire +2 more sources
, 2013 Human embryonic kidney cells 293 (HEK293) are widely used as cellular heterologous expression systems to study transfected ion channels. This work characterizes the endogenous expression of TRPM4 channels in HEK293 cells. TRPM4 is an intracellular Ca(2+)-
Amarouch, Mohamed Yassine +2 more
core +1 more source
, 2013 Cell lysates were prepared from HEK293/pcDNA3.1, HEK293/ABCB1 and HEK293/MRP7 cells (A), HEK293/MRP7 cells incubated with 5 µM BBA for different time periods (0, 24, 48 and 72 h) (B).
Atish Patel (285161) +10 more
core +1 more source
Molecular Oncology, EarlyView.EXOSC10, an essential nuclear RNA exosome‐associated 3′‐5′ exoribonuclease, is inhibited by the anticancer drug 5‐fluorouracil (5‐FU), and EXOSC10 depletion increases 5‐FU sensitivity. The colon‐cancer variant EXOSC10S402T, located in a proteolysis motif, is stable and nuclear but nonfunctional in vivo.
Radhika Sain +10 more
wiley +1 more source
Orai1 Expression, Mitosis and Cell Cycle Progression in HEK293 Cells [PDF]
Biophysical Journal, 2010 Calcium influx is needed for cell proliferation and recent identification of Orai1 as the main constituent for both store-operated (SOCE) and non capacitative (NCCE) calcium entries led us to investigate the role of Orai1 in mitosis and cell cycle progression in HEK293 cells.
Borowiec, Anne-Sophie +8 more
openaire +1 more source
Molecular Oncology, EarlyView.Here, we demonstrate that HS1BP3 interacts with Cortactin through a proline‐rich region (PRR3.1) and show that this interaction, and HS1BP3 itself, promote cancer cell proliferation and invasion. Inhibition of this interaction leads to build‐up of TKS5 in multivesicular endosomes and altered secretion of CD63 and CD9, providing an explanation for the ...
Arja Arnesen Løchen +9 more
wiley +1 more source
Nanomaterials, 2019 Graphene, a two-dimensional carbon sheet with single-atom thickness, shows immense promise in several nanoscientific and nanotechnological applications, including in sensors, catalysis, and biomedicine.
Sangiliyandi Gurunathan +12 more
doaj +1 more source
FEBS Open Bio, EarlyView.Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane +11 more
wiley +1 more source
Patulin suppresses α1-adrenergic receptor expression in HEK293 cells [PDF]
Scientific Reports, 2020 AbstractPatulin (PAT) is a common mycotoxin contaminant of apple products linked to impaired metabolic and kidney function. Adenosine monophosphate activated protein kinase (AMPK), abundantly expressed in the kidney, intercedes metabolic changes and renal injury.
Pillay, Yashodani +4 more
openaire +3 more sources