Results 61 to 70 of about 194,330 (301)

Rabies virus-like particles expressed in HEK293 cells

Vaccine, 2014
Rabies is an infectious viral disease with a mortality rate close to 100%. Currently, there is a need to generate cheaper and more immunogenic vaccines for the effective prevention of rabies, mostly in developing countries. Virus-like particles have been widely used in viral vaccine production due to their high immunogenicity and safety during the ...
Fontana, Diego Sebastian, Kratje, Ricardo Bertoldo, Etcheverrigaray, Marina, Prieto, Claudio   +3 more
openaire   +3 more sources

Production and Purification of Zika Virus NS1 Glycoprotein in HEK293 Cells [PDF]

, 2020
In this chapter, we describe production and purification of the Zika virus NS1 glycoprotein in human embryonic kidney (HEK293T) cells at small, research laboratory scale. The expression of secreted NS1 (sNS1) and the C-terminal β-ladder domain in HEK293T cells were tested in a small-scale transfection before scaling up to a larger-scale transfection ...
Kim, YC, Garcia-Larragoiti, N, Lopez-Camacho, C, Viveros-Sandoval, ME, Reyes-Sandoval, A   +4 more
openaire   +2 more sources

Orai1 Expression, Mitosis and Cell Cycle Progression in HEK293 Cells [PDF]

Biophysical Journal, 2010
Calcium influx is needed for cell proliferation and recent identification of Orai1 as the main constituent for both store-operated (SOCE) and non capacitative (NCCE) calcium entries led us to investigate the role of Orai1 in mitosis and cell cycle progression in HEK293 cells.
Borowiec, Anne-Sophie, El Boustany, Charbel, Katsogiannou, Maria, Bidaux, Gabriel, Delcourt, Philippe, Dewailly, Etienne, Slomianny, Christian, Prevarskaya, Natalia, Capiod, Thierry   +8 more
openaire   +1 more source

Potential therapeutic targeting of BKCa channels in glioblastoma treatment

Molecular Oncology, EarlyView.
This review summarizes current insights into the role of BKCa and mitoBKCa channels in glioblastoma biology, their potential classification as oncochannels, and the emerging pharmacological strategies targeting these channels, emphasizing the translational challenges in developing BKCa‐directed therapies for glioblastoma treatment.
Kamila Maliszewska‐Olejniczak, Karolina Pytlak, Sandra Jaworowska, Bogusz Kulawiak, Piotr Bednarczyk   +4 more
wiley   +1 more source

Interaction of HS1BP3 with cortactin modulates TKS5 localisation, cell secretion and cancer malignancy

Molecular Oncology, EarlyView.
Here, we demonstrate that HS1BP3 interacts with Cortactin through a proline‐rich region (PRR3.1) and show that this interaction, and HS1BP3 itself, promote cancer cell proliferation and invasion. Inhibition of this interaction leads to build‐up of TKS5 in multivesicular endosomes and altered secretion of CD63 and CD9, providing an explanation for the ...
Arja Arnesen Løchen, Kristiane Søreng, Chiara Veroni, Laura Trachsel‐Moncho, Nagham Asp, Robin Gaupset, Lars Gustav Lyckander, Helene Knævelsrud, Lars Eftang, Anne Simonsen   +9 more
wiley   +1 more source

Patulin suppresses α1-adrenergic receptor expression in HEK293 cells [PDF]

Scientific Reports, 2020
AbstractPatulin (PAT) is a common mycotoxin contaminant of apple products linked to impaired metabolic and kidney function. Adenosine monophosphate activated protein kinase (AMPK), abundantly expressed in the kidney, intercedes metabolic changes and renal injury.
Pillay, Yashodani, Nagiah, Savania, Phulukdaree, Alisa, Krishnan, Anand, Chuturgoon, Anil A.   +4 more
openaire   +3 more sources

Anchorage‐independent and faster growth in clonal population from UV‐irradiated NER‐deficient cells

FEBS Open Bio, EarlyView.
UV‐irradiated cells expressing a DDB2 mutant protein unable to interact with PCNA (DDB2PCNA‐) form clones able to grow without anchorage. Different experimental approaches reveal heterogeneity in cell cycle regulation and drug response within these clones, emphasizing the crucial role of the DDB2‐PCNA interaction in preventing cellular transformation ...
Paola Perucca, Anna Tricarico, Martina Furfaro, Priyam Ghosh, Ennio Prosperi, Lucia Anna Stivala, Ornella Cazzalini   +6 more
wiley   +1 more source

Evaluation of Graphene Oxide Induced Cellular Toxicity and Transcriptome Analysis in Human Embryonic Kidney Cells

Nanomaterials, 2019
Graphene, a two-dimensional carbon sheet with single-atom thickness, shows immense promise in several nanoscientific and nanotechnological applications, including in sensors, catalysis, and biomedicine.
Sangiliyandi Gurunathan, Muhammad Arsalan Iqbal, Muhammad Qasim, Chan Hyeok Park, Hyunjin Yoo, Jeong Ho Hwang, Sang Jun Uhm, Hyuk Song, Chankyu Park, Jeong Tae Do, Youngsok Choi, Jin-Hoi Kim, Kwonho Hong   +12 more
doaj   +1 more source

Meta‐analysis fails to show any correlation between protein abundance and ubiquitination changes

FEBS Open Bio, EarlyView.
We analyzed over 50 published proteomics datasets to explore the relationship between protein levels and ubiquitination changes across multiple experimental conditions and biological systems. Although ubiquitination is often associated with protein degradation, our analysis shows that changes in ubiquitination do not globally correlate with changes in ...
Nerea Osinalde, Unai Alduntzin, Gorka Prieto, Ugo Mayor   +3 more
wiley   +1 more source

Deconvolution of complex G protein-coupled receptor signaling in live cells using dynamic mass redistribution measurements [PDF]

, 2010
Label-free biosensor technology based on dynamic mass redistribution (DMR) of cellular constituents promises to translate GPCR signaling into complex optical 'fingerprints' in real time in living cells.
Blattermann, S, Drewke, C., Gomeza, J., Hennen, S., Janssen, N., Kebig, A., Kostenis, E., Merten, N., Milligan, G., Mohr, K., Mohr-Andra, M., Muller, A., Schmidt, J., Schroder, R., Smith, N.J., Wenzel, J., Zahn, S.   +16 more
core   +1 more source