Results 71 to 80 of about 4,870,076 (306)
Molecular Oncology, EarlyView.Isocitrate dehydrogenase 1 (IDH1) mutations are highly recurrent in multiple human cancer types, including cholangiocarcinoma and glioma. IDH1 R132C is the most common IDH1 mutation in cholangiocarcinoma and likely arises from APOBEC3A‐ or APOBEC3B‐mediated deamination.
Kelly E. Butler +3 more
wiley +1 more source
Substance P induces rapid and transient membrane blebbing in U373MG cells in a p21-activated kinase-dependent manner. [PDF]
PLoS ONE, 2011 U373MG astrocytoma cells endogenously express the full-length neurokinin 1 receptor (NK1R). Substance P (SP), the natural ligand for NK1R, triggers rapid and transient membrane blebbing and we report that these morphological changes have different ...
John Meshki +4 more
doaj +1 more source
Clusterin purification from HEK293E cells v1 [PDF]
, 2021 This protocol details the preocedure of clusterin purification from HEK293E cells.
openaire +1 more source
, 2013 Results revealed that OGD reduced mitochondrial activity and cell viability in HEK293 and HEK293DOR after OGD. Exposure to moderate hypothermia alone, DADLE or combination thereof led to neuroprotection, which was best manifested in HEK293DOR cells. MTT:
Yuji Kaneko (106226) +4 more
core +1 more source
Targeting the EpCAM‐AXL axis to overcome drug resistance in lung cancer
Molecular Oncology, EarlyView.Lung cancer cells often evade therapy by hijacking signaling pathways. We reveal that cleaved EpCAM (sEpCAM) stabilizes the oncogenic protein AXL, driving NF‐κB and STAT3‐mediated chemoresistance. This EpCAM‐AXL axis identifies a high‐risk patient subset with poor prognosis.
Alexa Guerrero‐Alba +5 more
wiley +1 more source
A Multi-Omics Analysis of Recombinant Protein Production in Hek293 Cells
PLoS ONE, 2012 Hek293 cells are the predominant hosts for transient expression of recombinant proteins and are used for stable expression of proteins where post-translational modifications performed by CHO cells are inadequate. Nevertheless, there is little information available on the key cellular features underpinning recombinant protein production in Hek293 cells.
Dietmair, Stefanie +5 more
openaire +7 more sources
Deciphering µ‐opioid receptor phosphorylation and dephosphorylation in HEK293 cells [PDF]
British Journal of Pharmacology, 2012 BACKGROUND AND PURPOSEThe molecular basis of agonist‐selective signalling at the µ‐opioid receptor is poorly understood. We have recently shown that full agonists such as [D‐Ala2‐MePhe4‐Gly‐ol]enkephalin (DAMGO) stimulate the phosphorylation of a number of carboxyl‐terminal phosphate acceptor sites including threonine 370 (Thr370) and serine 375 ...
Christian, Doll +5 more
openaire +2 more sources
, 2013 Nude mice were inoculated with MZ2-MEL cells in the right dorsal hip. After about 20 days, when the tumour was about 1–1.5×1–1.5 cm size, HEK293-pmeLUC (A) or HEK293-cytLUC (B) cells were injected s.c.
Lizzia Raffaghello (124257) +5 more
core +1 more source
FEBS Open Bio, EarlyView.Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya +2 more
wiley +1 more source
, 2014 A. Flow cytometry data on HEK293/B7/gfp and HEK293/F7/gfp cells 24h and 48 h after heat shock at 40 and 42°C. The proportion of GFP-positive HEK293/F7/gfp cells 24 and 48 h after heat shock was significantly higher than the control at 37°C ...
Ekaterina Savchenko (610852) +5 more
core +1 more source