Results 61 to 70 of about 132,829 (164)

Gene expression profiling analysis of bisphenol A-induced perturbation in biological processes in ER-negative HEK293 cells.

PLoS ONE, 2014
Bisphenol A (BPA) is an environmental endocrine disruptor which has been detected in human bodies. Many studies have implied that BPA exposure is harmful to human health.
Rong Yin, Liang Gu, Min Li, Cizhong Jiang, Tongcheng Cao, Xiaobai Zhang   +5 more
doaj   +1 more source

Use of Short-Lived Green Fluorescent Protein for the Detection of Proteasome Inhibition

BioTechniques, 2001
Human embryonic kidney (HEK293) cells were stably transduced with a retroviral vector containing an expression cassette for a short-lived green fluorescent protein (d2EGFP) and the neomycin resistance gene (Neor).
C. Andreatta, P. Nahreini, A.R. Hovland, B. Kumar, J. Edwards-Prasad, K.N. Prasad   +5 more
doaj   +1 more source

Tissue culture protocol for HEK293 cells v1

Lewis lab tissue culture protocol for HEK293 cells.
Susanne Herbst, Patrick Lewis
openaire   +1 more source

GAPDH suppresses adenovirus-induced oxidative stress and enables a superfast production of recombinant adenovirus

Genes and Diseases
Recombinant adenovirus (rAdV) is a commonly used vector system for gene transfer. Efficient initial packaging and subsequent production of rAdV remains time-consuming and labor-intensive, possibly attributable to rAdV infection-associated oxidative ...
Guozhi Zhao, Piao Zhao, Yonghui Wang, Hui Zhang, Yi Zhu, Jiamin Zhong, Wulin You, Guowei Shen, Changqi Luo, Ou Mei, Xingye Wu, Jingjing Li, Yi Shu, Hongwei Wang, William Wagstaff, Hue H. Luu, Yang Bi, Lewis L. Shi, Russell R. Reid, Tong-Chuan He, Li Jiang, Wei Tang, Jiaming Fan, Ziwei Tang   +23 more
doaj   +1 more source

Neurokinin 1 receptor mediates membrane blebbing and sheer stress-induced microparticle formation in HEK293 cells.

PLoS ONE, 2012
Cell-derived microparticles participate in intercellular communication similar to the classical messenger systems of small and macro-molecules that bind to specialized membrane receptors. Microparticles have been implicated in the regulation of a variety
Panpan Chen, Steven D Douglas, John Meshki, Florin Tuluc   +3 more
doaj   +1 more source

Construction of HEK293 and C2C12 cell models transfected by LMNA mutant and related intracellular sublocalization of lamin A/C [PDF]

Jichu yixue yu linchuang, 2020
Objective To construct an expression vector of fusion protein from wild type LMNA and mutant and the lentivirus vector of LMNA mutant, to study the expression and sublocalization of lamin A/C and the change of nucleus in HEK293 and C2C12 cells.
TAN Dan-dan, CHAI Jing-yan, LIU Jian-yun, NIE Hong-bing, XIONG Hui, WU Xiang-bin
doaj  

Keeping all secondary structures of the non-coding region in the circular genome of human bocavirus 2 is important for DNA replication and virus assembly, as revealed by three hetero-recombinant genomic clones

Emerging Microbes and Infections, 2019
The episomal structures of all human bocavirus (HBoV) genotypes have been deciphered, including the circular genome of HBoV2 (HBoV2-C1). To discern the role of the circular HBoV2 genome, three distinct linearized HBoV2-C1 genomes were cloned into ...
Linqing Zhao, Tao Wang, Yuan Qian, Jingdong Song, Runan Zhu, Liying Liu, Liping Jia, Huijin Dong   +7 more
doaj   +1 more source

Volume sensitive efflux of taurine in HEK293 cells overexpressing phospholemman

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2000
The role of the phospholemman (PLM) on the efflux of taurine and chloride induced by swelling was studied in HEK293 cells overexpressing stable transfected PLM. PLM, a substrate for protein kinases C and A, is a protein that induces an anion current in Xenopus oocytes and forms taurine-selective channels in lipid bilayers.
Morales-Mulia, Marcela, Pasantes-Morales, Herminia, Morán, Julio   +2 more
openaire   +2 more sources

Engineered AAV2.7m8 Serotype Shows Significantly Higher Transduction Efficiency of ARPE-19 and HEK293 Cell Lines Compared to AAV5, AAV8 and AAV9 Serotypes

Pharmaceutics
The level of transduction efficiency of the target retinal cells affects the choice of AAV serotype and the outcome of gene replacement therapy for inherited retinal diseases.
Dzerassa Gurtsieva, Ekaterina Minskaia, Sofia Zhuravleva, Elena Subcheva, Elena Sakhibgaraeva, Andrew Brovin, Artem Tumaev, Alexander Karabelsky   +7 more
doaj   +1 more source

The Effect of Aspirin and Ibuprofen on the Proliferation of Cervical Cancer Cells (HeLa) Compared to Non-Cancerous Cells (HEK 293) in Cell Culture Medium

Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum, 2018
Background and Objectives: Studies have shown that anti-inflammatory drugs can affect growth and development of cancer. This study was carried out to determine the effects of aspirin and ibuprofen on viability of cervical cancer cells (HeLa) compared to ...
Rahim Ahmadi, Zahra Karimi Ghezeli, Foroozan Gravand, Mahin Naghshineh   +3 more
doaj