Results 111 to 120 of about 194,742 (384)
mBio, 2021 Circular Rep-encoding single-stranded DNA (CRESS-DNA) viruses infect members from all three domains of life (Archaea, Prokarya, and Eukarya). The replicase (Rep) from these viruses is responsible for initiating rolling circle replication (RCR) of their ...
Elvira Tarasova +4 more
doaj +1 more source
Structure of the eukaryotic replicative CMG helicase and pumpjack motion
Nature Structural &Molecular Biology, 2016 The CMG helicase is composed of Cdc45, Mcm2–7 and GINS. Here we report the structure of the Saccharomyces cerevisiae CMG, determined by cryo-EM at a resolution of 3.7–4.8 Å.
Zuanning Yuan +6 more
semanticscholar +1 more source
Human Schlafen 14 Cleavage of Short Double‐Stranded RNAs Underpins its Antiviral Activity
Advanced Science, EarlyView.SLFN14 is associated with human diseases. SLFN14 is found to cleave RNAs containing short duplexes. The cryo‐EM structures of SLFN14 and SLFN14‐hairpin RNA complex reveal that SLFN14 assembles into a ring‐like dimer; two RNase domains form an RNA‐binding groove accommodating a hairpin RNA.
Mengyun Li +8 more
wiley +1 more source
Primase is required for helicase activity and helicase alters the specificity of primase in the enteropathogen Clostridium difficile [PDF]
Open Biology, 2016 DNA replication is an essential and conserved process in all domains of life and may serve as a target for the development of new antimicrobials. However, such developments are hindered by subtle mechanistic differences and limited understanding of DNA ...
Erika van Eijk +8 more
doaj +1 more source
Advanced Science, EarlyView.A rapid, portable lateral flow strip assay for on‐site mRNA quality control is developed, enabling sequence‐independent analysis of 5' capping, integrity, and LNPs encapsulation efficiency. This cost‐effective method offers real‐time stability monitoring, reduced sample requirements, and comparable accuracy to standard techniques, enhancing ...
Dengwang Luo +9 more
wiley +1 more source
Frontiers in Bioscience, 2012 Superfamily 2 helicases are involved in all aspects of RNA metabolism, and many steps in DNA metabolism. This review focuses on the basic mechanistic, structural and biological properties of each of the families of helicases within superfamily 2. There are ten separate families of helicases within superfamily 2, each playing specific roles in nucleic
Kevin D. Raney, Alicia K. Byrd
openaire +3 more sources
Active and passive mechanisms of helicases [PDF]
Nucleic Acids Research, 2010 In this work, we discuss the active or passive character of helicases. In the past years, several studies have used the theoretical framework proposed by Betterton and Julicher [Betterton, M.D. and Julicher, F. (2005) Opening of nucleic-acid double strands by helicases: active versus passive opening. Phys. Rev.
Manosas, Maria +3 more
openaire +4 more sources
Velocity and processivity of helicase unwinding of double-stranded nucleic acids
, 2005 Helicases are molecular motors which unwind double-stranded nucleic acids (dsNA) in cells. Many helicases move with directional bias on single-stranded (ss) nucleic acids, and couple their directional translocation to strand separation.
Bhattacharjee S M +9 more
core +1 more source
USP8‐Governed MDA5 Homeostasis Promotes Innate Immunity and Autoimmunity
Advanced Science, EarlyView.This study reveals that USP8 stabilizes MDA5 via AKT‐mediated phosphorylation (Ser718), enhancing their interaction and MDA5 deubiquitination. USP8 inactivation degrades MDA5, suppressing type I IFN and cytokine production. Pharmacological inhibition of USP8/AKT alleviates MDA5‐driven autoimmunity, demonstrating the USP8‐MDA5 axis as a therapeutic ...
Qimin Zhang +9 more
wiley +1 more source
The NSP5, ORF6 and NSP13 of SARS‐CoV‐2 Cooperate to Modulate Inflammatory Cell Death Activation
Advanced Science, EarlyView.ZBP1 is capable of initiating a large cell death complex to induce programmed cell death during SARS‐CoV‐2 infection. However, SARS‐CoV‐2 can inhibit the activation of ZBP1‐mediated cell death by targeting key components of this complex. This suppression of ZBP1‐mediated cell death may account for the increased mortality observed in patients co ...
Huan Wang +16 more
wiley +1 more source