Results 21 to 30 of about 132,729 (299)
ATPase mechanism of the 5'-3' DNA helicase, RecD2: evidence for a pre-hydrolysis conformation change [PDF]
, 2013 The superfamily 1 helicase, RecD2, is a monomeric, bacterial enzyme with a role in DNA repair, but with 5'-3' activity unlike most enzymes from this superfamily. Rate constants were determined for steps within the ATPase cycle of RecD2 in the presence of
Webb, Martin R, Toseland, Christopher P.
core +1 more source
Recognition of two distinct elements in the RNA substrate by the RNA-binding domain of the T. thermophilus DEAD box helicase Hera [PDF]
, 2013 DEAD box helicases catalyze the ATP-dependent destabilization of RNA duplexes. Whereas duplex separation is mediated by the helicase core shared by all members of the family, flanking domains often contribute to binding of the RNA substrate.
Wöhnert, Jens +5 more
core +1 more source
Vaccines, 2021 In response to the ongoing COVID-19 pandemic, the global effort to develop high efficacy countermeasures to control the infection are being conducted at full swing.
Irfan A. Rather +6 more
doaj +1 more source
EcoSal Plus, 2010 DNA and RNA helicases are organized into six superfamilies of enzymes on the basis of sequence alignments, biochemical data, and available crystal structures. DNA helicases, members of which are found in each of the superfamilies, are an essential group of motor proteins that unwind DNA duplexes into their component single strands in a ...
openaire +2 more sources
Domain orientation in the RNA helicase YxiN and the role of conformational changes for RNA unwinding [PDF]
, 2010 The RNA helicase YxiN from Bacillus subtilis is a member of the family of DEAD box proteins. YxiN is able to unwind RNA double strands in an ATP-dependent manner. The ability to catalyse RNA rearrangement is in vivo presumably necessary for the bacterial
Karow, Anne R.
core +1 more source
Biochemical characterization of Plasmodium falciparum parasite specific helicase 1 (PfPSH1)
FEBS Open Bio, 2019 Malaria, a disease caused by infection with parasites of the genus Plasmodium, causes millions of deaths worldwide annually. Of the five Plasmodium species that can infect humans, Plasmodium falciparum causes the most serious parasitic infection.
Manish Chauhan +4 more
doaj +1 more source
Biochemical Characterization of Middle East Respiratory Syndrome Coronavirus Helicase
mSphere, 2016 Middle East respiratory syndrome coronavirus (MERS-CoV) helicase is a superfamily 1 helicase containing seven conserved motifs. We have cloned, expressed, and purified a Strep-fused recombinant MERS-CoV nonstructural protein 13 (M-nsp13) helicase ...
Adeyemi O. Adedeji, Hilary Lazarus
doaj +1 more source
Structure of the Helicase Domain of DNA Polymerase Theta Reveals a Possible Role in the Microhomology-Mediated End-Joining Pathway [PDF]
, 2015 DNA polymerase theta (Polθ) has been identified as a crucial alternative non-homologous end-joining factor in mammalian cells. Polθ is upregulated in a range of cancer cell types defective in homologous recombination, and knockdown has been shown to ...
Aitkenhead, H +8 more
core +1 more source
Structural and Functional Studies of Chikungunya Virus nsP2
Proceedings, 2020 Chikungunya virus (CHIKV) is transmitted to humans through mosquitoes and causes Chikungunya fever. Nonstructural protein 2 (nsP2) contains an N-terminal RNA helicase with both nucleotide triphosphatase and RNA triphosphatase activities, and a C-terminal
Yee Song Law +10 more
doaj +1 more source
Viruses, 2022 Hepatitis E virus (HEV) is a major cause of acute viral hepatitis in humans globally. Considered for a long while a public health issue only in developing countries, the HEV infection is now a global public health concern.
Adeline Goulet +3 more
doaj +1 more source