Results 71 to 80 of about 134,478 (313)

Expression profiling of snoRNAs in normal hematopoiesis and AML [PDF]

, 2018
Key Points A subset of snoRNAs is expressed in a developmental- and lineage-specific manner during human hematopoiesis. Neither host gene expression nor alternative splicing accounted for the observed differential expression of snoRNAs in ...
Helton, Nichole, Ley, Timothy J., Link, Daniel C., Spencer, David H., Trissal, Maria, Warner, Wayne A., White, Brian S.   +6 more
core   +2 more sources

Targeting rapid TKI‐induced AXL upregulation overcomes adaptive ERK reactivation and exerts antileukemic effects in FLT3/ITD acute myeloid leukemia

Molecular Oncology, EarlyView.
Adaptive ERK reactivation hinders FLT3 tyrosine kinase inhibitor (TKI) treatment in FLT3/ITD acute myeloid leukemia. Here, we report that FLT3 TKI treatment rapidly induces AXL expression and upregulation that is temporally associated with the adaptive ERK reactivation.
Tessa S. Seale, Li Li, J. Kyle Bruner, Melody Chou, Bao Nguyen, Jaesung Seo, Ruiqi Zhu, Mark J. Levis, Christine A. Pratilas, Donald Small   +9 more
wiley   +1 more source

Differentiating erythroblasts adapt to mechanical stimulation by upregulation of cholesterol biosynthesis via S1P/SREBP-induced HMGCR expression

Scientific Reports
Understanding how mechanical stress affects erythropoiesis is crucial to produce transfusable erythrocytes in fluid-turbulent bioreactors. We investigated the effects of shear-stress on differentiating CD49d+CD235a+ primary human erythroblasts (EBL) at ...
Giulia Iacono, Asena Abay, Joan S. Gallego Murillo, Francesca Aglialoro, Nurcan Yagci, Eszter Varga, Tieme Bijlsma, Justine Sohler, Kerly Fu, Julie A. Reisz, Amy Argabright, Angelo D’Alessandro, Arthur F. Svendsen, Marieke von Lindern, Emile van den Akker   +14 more
doaj   +1 more source

Circulating tumor DNA (ctDNA) trajectories predict survival in trifluridine/tipiracil‐treated metastatic colorectal cancer patients

Molecular Oncology, EarlyView.
The authors applied joint/mixed models that predict mortality of trifluridine/tipiracil‐treated metastatic colorectal cancer patients based on circulating tumor DNA (ctDNA) trajectories. Patients at high risk of death could be spared aggressive therapy with the prospect of a higher quality of life in their remaining lifetime, whereas patients with a ...
Matthias Unseld, Stefan Kühberger, Ricarda Graf, Christine Beichler, Markus Braun, Nadia Dandachi, Ellen Heitzer, Gerald W. Prager   +7 more
wiley   +1 more source

Generation and characterization of human iPSC lines SANi001-A and SANi002-A from mobilized peripheral blood derived megakaryoblasts

Stem Cell Research, 2017
Mobilized peripheral blood (MPB) CD34+ cells were differentiated to CD34+/CD41+ megakaryoblasts. Cells were sorted to obtain a pure megakaryoblast population which was reprogrammed with a hOKSM self-silencing polycistronic lentiviral vector.
Marten Hansen, Eszter Varga, Tatjana Wüst, Clemens Mellink, Anne-Marie van der Kevie-Kersemaekers, Marieke von Lindern, Emile van den Akker   +6 more
doaj   +1 more source

Hemoglobin switching in mice carrying the Klf1^Nan variant

Haematologica, 2020
Haploinsufficiency for transcription factor KLF1 causes a variety of human erythroid phenotypes, such as the In(Lu) blood type, increased HbA2 levels, and hereditary persistence of fetal hemoglobin. Severe dominant congenital dyserythropoietic anemia IV
Anne Korporaal, Nynke Gillemans, Steven Heshusius, Ileana Cantú, Emile van den Akker, Thamar B. van Dijk, Marieke von Lindern, Sjaak Philipsen   +7 more
doaj   +1 more source

Robust acute myeloid leukemia engraftment in humanized scaffolds using injectable biomaterials and intravenous xenotransplantation

Molecular Oncology, EarlyView.
Patient‐derived xenografts (PDXs) can be improved by implantation of a humanized niche. We tested different biomaterials and approaches, and demonstrate that the combination of an injectable biomaterial for scaffold creation plus an intravenous route for acute myeloid leukemia (AML) xenotransplantation provide the most convenient and robust approach to
Daniel Busa, Zdenka Herudkova, Jan Hyl, Jakub Vlazny, Filip Sokol, Kvetoslava Matulova, Adam Folta, Jakub Hynst, Lucy Vojtova, Leos Kren, Martin Repko, Zdenek Racil, Jiri Mayer, Martin Culen   +13 more
wiley   +1 more source

Isoform-specific potentiation of stem and progenitor cell engraftment by AML1/RUNX1 [PDF]

, 2007
Background: AML1/RUNX1 is the most frequently mutated gene in leukaemia and is central to the normal biology of hematopoietic stem and progenitor cells. However, the role of different AML1 isoforms within these primitive compartments is unclear.
Ailles, Bae, Blyth, Brunstein, Cai, Calkhoven, Cantor, Cashman, Chickarmane, Christiansen, de Bruijn, de Guzman, de Wynter, Demaison, Dengli Hong, Elaine Dzierzak, Freyssinier, Friedman, Fujita, Growney, Guenechea, Harada, Ichikawa, Imai, Ito, Itoh, Jin, Kasugai, Keitaro Matsuo, Levanon, Ling, Masao Seto, Miyoshi, Miyoshi, Mulloy, Nakase, Nishihara, Nishimura, Niwa, North, North, O&apos, Ohkawara, Okuda, Okuda, Osato, Persons, Piacibello, Putz, Rajeev Gupta, Reif, Rodrigues, Sasaki, Schwieger, Shinobu Tsuzuki, Song, Sorrentino, Speck, Stirewalt, Sun, Sun, Swiers, Tanaka, Tariq Enver, Taussig, Tonnelle, Tsuzuki, Wang, Wechsler, Xu, Yagi, Yin, Zhang   +72 more
core   +3 more sources

CRISPR targeting of FOXL2 c.402C>G mutation reduces malignant phenotype in granulosa tumor cells and identifies anti‐tumoral compounds

Molecular Oncology, EarlyView.
FOXL2 c.402C>G mutation drives granulosa cell tumors. Using CRISPR technology, we selectively corrected this mutation, reducing malignancy and increasing sensitivity to dasatinib and ketoconazole. Transcriptomic changes revealed potential therapeutic targets, demonstrating CRISPR's promise for treating this rare ovarian cancer.
Sandra Amarilla‐Quintana, Paloma Navarro, Iván Hernández, Alejandra Ramos, Ana Montero‐Calle, Pablo Cabezas‐Sainz, Maria J Barrero, Diego Megías, Borja Vilaplana‐Martí, Carolina Epifano, Déborah Gómez‐Dominguez, Sara Monzón, Isabel Cuesta, Laura Sánchez, Rodrigo Barderas, Jesús García‐Donas, Alberto Martín, Ignacio Pérez de Castro   +17 more
wiley   +1 more source

Improved Human Erythropoiesis and Platelet Formation in Humanized NSGW41 Mice

Stem Cell Reports, 2016
Human erythro-megakaryopoiesis does not occur in humanized mouse models, preventing the in vivo analysis of human hematopoietic stem cell (HSC) differentiation into these lineages in a surrogate host.
Susann Rahmig, Romy Kronstein-Wiedemann, Juliane Fohgrub, Nicole Kronstein, Aleksandra Nevmerzhitskaya, Martin Bornhäuser, Max Gassmann, Alexander Platz, Rainer Ordemann, Torsten Tonn, Claudia Waskow   +10 more
doaj   +1 more source