Results 1 to 10 of about 292,553 (270)

Combined proteomics and CRISPR‒Cas9 screens in PDX identify ADAM10 as essential for leukemia in vivo

Molecular Cancer, 2023
Background Acute leukemias represent deadly malignancies that require better treatment. As a challenge, treatment is counteracted by a microenvironment protecting dormant leukemia stem cells. Methods To identify responsible surface proteins, we performed
Ehsan Bahrami, Jan Philipp Schmid, Vindi Jurinovic, Martin Becker, Anna-Katharina Wirth, Romina Ludwig, Sophie Kreissig, Tania Vanessa Duque Angel, Diana Amend, Katharina Hunt, Rupert Öllinger, Roland Rad, Joris Maximilian Frenz, Maria Solovey, Frank Ziemann, Matthias Mann, Binje Vick, Christian Wichmann, Tobias Herold, Ashok Kumar Jayavelu, Irmela Jeremias   +20 more
doaj   +1 more source

A reporter system for enriching CRISPR/Cas9 knockout cells in technically challenging settings like patient models

Scientific Reports, 2021
CRISPR/Cas9 represents a valuable tool to determine protein function, but technical hurdles limit its use in challenging settings such as cells unable to grow in vitro like primary leukemia cells and xenografts derived thereof (PDX).
Wen-Hsin Liu, Kerstin Völse, Daniela Senft, Irmela Jeremias   +3 more
doaj   +1 more source

Aging of hematopoietic stem cells [PDF]

Blood, 2018
AbstractHematopoietic stem cells (HSCs) ensure a balanced production of all blood cells throughout life. As they age, HSCs gradually lose their self-renewal and regenerative potential, whereas the occurrence of cellular derailment strongly increases. Here we review our current understanding of the molecular mechanisms that contribute to HSC aging.
Gerald de Haan, Seka Simone Lazare
openaire   +3 more sources

Hematopoietic Stem Cells [PDF]

, 2006
Hematopoietic stem cells (HSCs) have the capacity to self-renew and the potential to differentiate into all of the mature blood cell types. The ability to prospectively identify and isolate HSCs has been the subject of extensive investigation since the first transplantation studies implying their existence almost 50 years ago.
Robert G, Hawley, Ali, Ramezani, Teresa S, Hawley   +2 more
openaire   +2 more sources

IFNα-mediated remodeling of endothelial cells in the bone marrow niche

Haematologica, 2017
In the bone marrow, endothelial cells are a major component of the hematopoietic stem cell vascular niche and are a first line of defense against inflammatory stress and infection.
Áine M. Prendergast, Andrea Kuck, Mieke van Essen, Simon Haas, Sandra Blaszkiewicz, Marieke A. G. Essers   +5 more
doaj   +1 more source

DARS2 overexpression is associated with PET/CT metabolic parameters and affects glycolytic activity in lung adenocarcinoma

Journal of Translational Medicine, 2023
Background This study investigated the correlation between the expression of DARS2 and metabolic parameters of 18F-FDG PET/CT, and explored the potential mechanisms of DARS2 affecting the proliferation and glycolysis of lung adenocarcinoma (LUAD) cells ...
Xu-Sheng Liu, Ling-Ling Yuan, Yan Gao, Xing Ming, Yao-Hua Zhang, Yu Zhang, Zi-Yue Liu, Yi Yang, Zhi-Jun Pei   +8 more
doaj   +1 more source

Gene expression profiles and cytokine environments determine the in vitro proliferation and expansion capacities of human hematopoietic stem and progenitor cells

Hematology, 2022
Objective The interplay between intrinsic and extrinsic elements involved in the physiology of hematopoietic cells is not completely understood. In the present study, we analyzed the transcriptional profiles of human cord blood-derived hematopoietic stem
Roberto Dircio-Maldonado, Rosario Castro-Oropeza, Patricia Flores-Guzman, Alberto Cedro-Tanda, Fredy Omar Beltran-Anaya, Alfredo Hidalgo-Miranda, Hector Mayani   +6 more
doaj   +1 more source

Identification of the niche and phenotype of the first human hematopoietic stem cells [PDF]

, 2014
In various vertebrate species, the dorsal aorta (Ao) is the site of specification of adult hematopoietic stem cells (HSCs). It has been observed that the upregulation of essential hematopoietic transcription factors and the formation of specific intra ...
Stanislav Rybtsov, Anderson, Richard A., Turner, Marc L., Anderson, Richard A, Ivanovs, Andrejs, Andrejs Ivanovs, Richard A. Anderson, Turner, Marc L, Marc L. Turner, Alexander Medvinsky, Medvinsky, Alexander, Rybtsov, Stanislav   +11 more
core   +1 more source

Generation and characterization of erythroid cells from human embryonic stem cells and induced pluripotent stem cells : an overview [PDF]

, 2011
Because of the imbalance in the supply and demand of red blood cells (RBCs), especially for alloimmunized patients or patients with rare blood phenotypes, extensive research has been done to generate therapeutic quantities of mature RBCs from ...
Bönig, Halvard-Björn, Chang, Kai-Hsin, Kai-Hsin Chang, Thalia Papayannopoulou, Halvard Bonig, Papayannopoulou, Thalia   +5 more
core   +1 more source

Making a Hematopoietic Stem Cell [PDF]

Trends in Cell Biology, 2016
Previous attempts to either generate or expand hematopoietic stem cells (HSCs) in vitro have involved either ex vivo expansion of pre-existing patient or donor HSCs or de novo generation from pluripotent stem cells (PSCs), comprising both embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs).
Michael G, Daniel, Carlos-Filipe, Pereira, Ihor R, Lemischka, Kateri A, Moore   +3 more
openaire   +2 more sources