Results 101 to 110 of about 6,457,465 (175)

Insights into the dynamic cell associated and secreted proteome of Staphylococcus aureus cultured in TSB and milk media: a proteomic analysis

BMC Microbiology
The availability of nutrients is closely linked to the protein expression profile of microorganisms. In this study, a comparative investigation has been performed to examine the expression patterns of cell associated proteins and secreted proteins in ...
Wanting Zhu, Yujing Wang, Yanxin Li, Changjiang Zang, Hongning Jiang, Qijing Du, Jun Wang, Rongbo Fan, Rongwei Han, Yongxin Yang   +9 more
doaj   +1 more source

P2X-Receptor Antagonists Inhibit the Interaction of S. aureus Hemolysin A with Membranes

Toxins, 2017
The pore forming hemolysin A, Hla, is a major virulence factor of Staphylococcus aureus. Apparently, 1–2 pore(s) per cell suffice(s) to cause cell death.
Markus Schwiering, Matthias Husmann, Nadja Hellmann   +2 more
doaj   +1 more source

The Draft Genome Sequence of the Yersinia entomophaga Entomopathogenic Type Strain MH96T

Toxins, 2016
Here we report the draft genome of Yersinia entomophaga type strain MH96T. The genome shows 93.8% nucleotide sequence identity to that of Yersinia nurmii type strain APN3a-cT, and comprises a single chromosome of approximately 4,275,531 bp.
Mark R. H. Hurst, Amy Beattie, Eric Altermann, Roger M. Moraga, Lincoln A. Harper, Joanne Calder, Aurelie Laugraud   +6 more
doaj   +1 more source

FlhF is required for swarming motility and full pathogenicity of Bacillus cereus

Frontiers in Microbiology, 2016
Besides sporulation, Bacillus cereus can undergo a differentiation process in which short swimmer cells become elongated and hyperflagellated swarmer cells that favor migration of the bacterial community on a surface. The functionally enigmatic flagellar
Diletta Mazzantini, Francesco Celandroni, Sara Salvetti, Sokhna Aissatou Gueye, Antonella Lupetti, Sonia Senesi, Emilia Ghelardi, Emilia Ghelardi   +7 more
doaj   +1 more source

Engineered α-hemolysin pores with chemically and genetically-fused functional proteins

, 2016
Protein engineering could be used to bring two proteins together, which don't normally interact, in an oriented configuration. Using computer modelling and experimental work involving mutagenesis, a new dimer complex, (α7)2, was engineered with two α-hemolysin (αHL) heptamers (α7) units linked via disulfide bridges in a cap-to-cap orientation.
openaire   +3 more sources

Two forms of Vibrio cholerae O1 El Tor hemolysin derived from identical precursor protein

Biochimica et Biophysica Acta (BBA) - Biomembranes, 1999
Vibrio cholerae O1 grown in heart infusion broth produces two forms of El Tor hemolysin (ETH) monomers of 65 and 50 kDa. These monomers form several different sizes of mixed oligomers ranging from 180 to 280 kDa in the liposomal membranes. We found that the N-terminal amino acid sequences, NH2-Trp-Pro-Ala-Pro-Ala-Asn-Ser-Glu, of both the 65- and 50-kDa
Ikigai, Hajime, Ono, Toshihisa, Nakae, Taiji, Otsuru, Hiroshi, Shimamura, Tadakatsu   +4 more
openaire   +2 more sources

The anti-biofilm compound 4-ethoxybenzoic acid inhibits Staphylococcus aureus virulence factor production via a putative 4EB-binding pocket in key virulence-associated proteins

Frontiers in Microbiology
There is a need for dual action anti-virulence and anti-biofilm agents that target the opportunistic pathogen Staphylococcus aureus. Previous research determined that 0.8 mg/mL 4-ethoxybenzoic acid (4EB) reduced S.
Caroline C. Taylor, Adonis Aviles-Gonzalez, Alexander Marchesani, Christina Kiessling, Travis Patrick, Linxin Chen, Haozhe Yao, Zixuan Li, Abbie Seward, Kuk-Jeong Chin, Eric S. Gilbert   +10 more
doaj   +1 more source

Periodically driven force protein translocation through a α-hemolysin biological nano-pore

, 2018
We study the translocation of protein pulled under the action of time periodically external driving force through α-hemolysin nano-pore using Langevin molecular dynamical simulation. We observe that time depended external pulling force could enhance to more efficient translocation process as compared to protein translocation driven by constant external
openaire   +1 more source

The UPEC pore-forming toxin α-hemolysin triggers proteolysis of host proteins to disrupt cell adhesion, inflammatory, and survival pathways.

Cell Host and Microbe, 2012
B. Dhakal, M. A. Mulvey
semanticscholar   +1 more source

Encoding extracellular modification of artificial cell membranes using engineered self-translocating proteins

Nature Communications
The development of artificial cells has led to fundamental insights into the functional processes of living cells while simultaneously paving the way for transformative applications in biotechnology and medicine.
Alexander Harjung, Alessandro Fracassi, Neal K. Devaraj   +2 more
doaj   +1 more source