Results 71 to 80 of about 196,489 (314)
Molecular Chain Elongation Mechanism for n‐Caproate Biosynthesis by Megasphaera Hexanoica
Advanced Science, EarlyView.The medium‐chain carboxylate‐producing microbe Megasphaera hexanoica is analyzed through genomics, transcriptomics, and metabolic engineering. The n‐caproate biosynthetic pathway is reconstructed in Escherichia coli, highlighting MhTHL as a key β‐ketothiolase. Structural analysis and mutagenesis reveal that MhTHL has a distinct substrate‐binding pocket,
Byoung Seung Jeon +8 more
wiley +1 more source
Genetic Approaches to Reveal the Connectivity of Adult-Born Neurons
Frontiers in Neuroscience, 2011 Much has been learned about the environmental and molecular factors that influence the division, migration, and programmed cell death of adult-born neurons in the mammalian brain.
Benjamin eArenkiel
doaj +1 more source
Retrosynthetic Design of Heterologous Pathways
, 2013 Tools from metabolic engineering and synthetic biology are synergistically used in order to develop high-performance cell factories. However, the number of successful applications has been limited due to the complexity of exploring efficiently the metabolic space for the discovery of candidate heterologous pathways.
Carbonell, Pablo +2 more
openaire +4 more sources
Advanced Science, EarlyView.Understanding the virus‐host arms race has been a fascinating topic in virology; contributing significantly to the development of treatments and control strategies for viral diseases. CHV1 employs a sophisticated counter‐defense mechanism in its fungal host by utilizing a viral silencing suppressor to inhibit fungal FMN reductase; thereby modulating ...
Shiyu Zhai +8 more
wiley +1 more source
H2S Is a Potential Universal Reducing Agent for Prx6‐Type Peroxiredoxins
Advanced Science, EarlyView.We report the identification of H2S as reducing agent for Prx6‐type peroxiredoxins. In contrast to common physiological reducing agents of other peroxiredoxins, HS− is small enough to access the active site of oxidized Prx6‐type enzymes in the fully‐folded protein conformation.
Lukas Lang +3 more
wiley +1 more source
T and B cell responses in different immunization scenarios for COVID-19: a narrative review
Frontiers in ImmunologyVaccines against COVID-19 have high efficacy and low rates of adverse events. However, none of the available vaccines provide sterilizing immunity, and reinfections remain possible.
Eva Piano Mortari +4 more
doaj +1 more source
Advanced Science, EarlyView.The artificial biosynthetic pathway potentially involved in biosynthesis of enfumafungin is first reconstructed in an optimized Aspergillus oryzae chassis with the increased site‐specific integration efficiency and shortened time for marker recycling, which requires an artificial fusion enzyme EfuA(TC)FsoA(GT).
Yaohui Gao +11 more
wiley +1 more source
Frontiers in ImmunologyIntroductionMelioidosis is a major cause of disease and mortality in endemic tropical regions, and the etiologic agent, Burkholderia pseudomallei, is being isolated increasingly from an expanded range of environmental and clinical sources in locations ...
Sergei S. Biryukov +17 more
doaj +1 more source
Advanced Science, EarlyView.This study presents a refined adaptive laboratory evolution (ALE) strategy to resolve the trade‐off between tolerance and biosynthetic efficiency in toxic chemical production. Combining in vivo mutagenesis, microdroplet‐based evolution, and biosensor‐assisted selection, this strategy rapidly yields robust “win‐win” phenotypes.
Yufei Zhang +7 more
wiley +1 more source
Advanced Science, EarlyView.This study engineers Yarrowia lipolytica to produce succinic acid (SA) from lignocellulosic biomass. Adaptive evolution and multi‐omics analysis identify regulators that alleviate xylose metabolic disorder. Multi‐copy integration of xylose metabolic genes resolves redox imbalances from a futile fatty acid cycle, producing high‐titer SA from corn stover
Yutao Zhong +7 more
wiley +1 more source