Results 1 to 10 of about 145,939 (263)

A highly efficient heterologous expression platform to facilitate the production of microbial natural products in Streptomyces [PDF]

Microbial Cell Factories
Background Heterologous expression in Streptomyces provides a platform for mining natural products (NPs) encoded by cryptic biosynthetic gene clusters (BGCs) of bacteria.
Xiuling Wang, Ping Lin, Qiyao Shen, Xueyan Feng, Shouying Xu, Qijun Zhang, Yang Liu, Cailing Ren, Daojing Yong, Qiong Duan, Liujie Huo, Youming Zhang, Gang Li, Jun Fu, Ruijuan Li   +14 more
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Advance in Heterologous Expression of Biomass-Degrading Auxiliary Activity 10 Family of Lytic Polysaccharide Monooxygenases

Fermentation, 2023
AA10 family lytic polysaccharide monooxygenases (AA10 LPMOs) are mainly distributed in bacteria. Because of their characteristics of oxidative degradation of crystalline polysaccharides, such as cellulose and chitin, they have great application potential
Hongyu Zhang, Zixuan Zhou, Tingting Lou, Rong Xiang, Deguang Zhang, Danyun Wang, Suying Wang   +6 more
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Alternative sigma factor over-expression enables heterologous expression of a type II polyketide biosynthetic pathway in Escherichia coli. [PDF]

PLoS ONE, 2013
BACKGROUND: Heterologous expression of bacterial biosynthetic gene clusters is currently an indispensable tool for characterizing biosynthetic pathways.
David Cole Stevens, Kyle R Conway, Nelson Pearce, Luis Roberto Villegas-Peñaranda, Anthony G Garza, Christopher N Boddy   +5 more
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Heterologous expression of a fully active Azotobacter vinelandii nitrogenase Fe protein in Escherichia coli

mBio, 2023
The functional versatility of the Fe protein, the reductase component of nitrogenase, makes it an appealing target for heterologous expression, which could facilitate future biotechnological adaptations of nitrogenase-based production of valuable ...
Joseph B. Solomon, Yiling A. Liu, Kamil Górecki, Robert Quechol, Chi Chung Lee, Andrew J. Jasniewski, Yilin Hu, Markus W. Ribbe   +7 more
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Dudomycins: New Secondary Metabolites Produced after Heterologous Expression of an Nrps Cluster from Streptomyces albus ssp. Chlorinus Nrrl B-24108

Microorganisms, 2020
Since the 1950s, natural products of bacterial origin were systematically developed to be used as drugs with a wide range of medical applications. The available treatment options for many diseases are still not satisfying, wherefore, the discovery of new
Constanze Lasch, Marc Stierhof, Marta Rodríguez Estévez, Maksym Myronovskyi, Josef Zapp, Andriy Luzhetskyy   +5 more
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Reconstitution of Polyketide-Derived Meroterpenoid Biosynthetic Pathway in Aspergillus oryzae

Journal of Fungi, 2021
The heterologous gene expression system with Aspergillus oryzae as the host is an effective method to investigate fungal secondary metabolite biosynthetic pathways for reconstruction to produce un-natural molecules due to its high productivity and ...
Takayoshi Awakawa, Ikuro Abe
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Construction of T7-Like Expression System in Pseudomonas putida KT2440 to Enhance the Heterologous Expression Level

Frontiers in Chemistry, 2021
Pseudomonas putida KT2440 has become an attractive chassis for heterologous expression with the development of effective genetic manipulation tools. Improving the level of transcriptional regulation is particularly important for extending the potential ...
Tianxin Liang, Jun Sun, Shuyun Ju, Shuyun Ju, Shenyi Su, Lirong Yang, Lirong Yang, Jianping Wu, Jianping Wu   +8 more
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Xenopus Oocytes: A Tool to Decipher Molecular Specificity of Insecticides towards Mammalian and Insect GABA—A Receptors

Membranes, 2022
The number of insect GABA receptors (GABAr) available for expression studies has been recently increased by the cloning of the Acyrthosiphon pisum (pea aphid) RDL subunits.
Anaïs Bertaud, Thierry Cens, Rosanna Mary, Matthieu Rousset, Elodie Arel, Jean-Baptiste Thibaud, Michel Vignes, Claudine Ménard, Sébastien Dutertre, Claude Collet, Pierre Charnet   +10 more
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A set of vectors and strains for chromosomal integration in fission yeast

Scientific Reports, 2023
The expression of heterologous genes is an important technique in yeast genetics. In fission yeast, the leu1 and ura4 genes have been used mainly as selectable markers for heterologous expression.
Akihisa Matsuyama, Atsushi Hashimoto, Shinichi Nishimura, Minoru Yoshida   +3 more
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Recent Advances in the Heterologous Expression of Biosynthetic Gene Clusters for Marine Natural Products

Marine Drugs, 2022
Marine natural products (MNPs) are an important source of biologically active metabolites, particularly for therapeutic agent development after terrestrial plants and nonmarine microorganisms.
Yushan Xu, Xinhua Du, Xionghui Yu, Qian Jiang, Kaiwen Zheng, Jinzhong Xu, Pinmei Wang   +6 more
doaj   +1 more source