Results 11 to 20 of about 261,803 (304)
Modelling hCDKL5 Heterologous Expression in Bacteria [PDF]
Metabolites, 2021 hCDKL5 refers to the human cyclin-dependent kinase like 5 that is primarily expressed in the brain. Mutations in its coding sequence are often causative of hCDKL5 deficiency disorder, a devastating neurodevelopmental disorder currently lacking a cure ...
Marco Fondi +9 more
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Heterologous Expression of Functional Ptr ToxA [PDF]
Molecular Plant-Microbe Interactions, 2000 Ptr ToxA, a proteinaceous host-selective toxin (HST) produced by the fungus Pyrenophora tritici-repentis, was expressed in Escherichia coli and purified as a polyhistidine-tagged, fusion protein (NC-FP).
Robert P. Tuori +2 more
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Heterologous expression of human transketolase [PDF]
The International Journal of Biochemistry & Cell Biology, 1998 Transketolase belongs to the family of thiamin diphosphate dependent enzymes. The aim of this study was to establish a bacterial expression system for human transketolase in order to investigate the functional characteristics of mammalian transketolases. The level of recombinant human enzyme expressed in Escherichia coli was modest.
Schenk, Gerhard +2 more
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Heterologous Expression of Limulus Rhodopsin [PDF]
Journal of Biological Chemistry, 2003 Invertebrates such as Drosophila or Limulus assemble their visual pigment into the specialized rhabdomeric membranes of photoreceptors where phototransduction occurs. We have investigated the biosynthesis of rhodopsin from the Limulus lateral eye with three cell culture expression systems: mammalian COS1 cells, insect Sf9 cells, and amphibian Xenopus ...
Barry E, Knox +8 more
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A set of vectors and strains for chromosomal integration in fission yeast
Scientific Reports, 2023 The expression of heterologous genes is an important technique in yeast genetics. In fission yeast, the leu1 and ura4 genes have been used mainly as selectable markers for heterologous expression.
Akihisa Matsuyama +3 more
doaj +1 more source
Marine Drugs, 2022 Marine natural products (MNPs) are an important source of biologically active metabolites, particularly for therapeutic agent development after terrestrial plants and nonmarine microorganisms.
Yushan Xu +6 more
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A condition-specific codon optimization approach for improved heterologous gene expression in Saccharomyces cerevisiae [PDF]
, 2014 All authors are with the Department of Chemical Engineering, The University of Texas at Austin, 200 E Dean Keeton St. Stop C0400, Austin, TX 78712, USA -- Hal S.
Alper, Hal S. +3 more
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Expression of Heterologous OsDHAR Gene Improves Glutathione (GSH)-Dependent Antioxidant System and Maintenance of Cellular Redox Status in Synechococcus elongatus PCC 7942. [PDF]
, 2020 An excess of reactive oxygen species (ROS) can cause severe oxidative damage to cellular components in photosynthetic cells. Antioxidant systems, such as the glutathione (GSH) pools, regulate redox status in cells to guard against such damage ...
Beld, Joris +9 more
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Efficient Protein Expression and Virus-Induced Gene Silencing in Plants Using a Crinivirus-Derived Vector. [PDF]
, 2018 Plant virus-based vectors are valuable tools for recombinant gene expression and functional genomics for both basic and applied research. In this study, Lettuce infectious yellows virus (LIYV) of the genus Crinivirus was engineered into a virus vector ...
Falk, Bryce W, Qiao, Wenjie
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Frontiers in Microbiology, 2018 Streptomyces lividans is a suitable host for the heterologous expression of biosynthetic gene clusters (BGCs) from actinomycetes to discover “cryptic” secondary metabolites. To improve the heterologous expression of BGCs, herein we optimized S.
Qinying Peng +11 more
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