Results 21 to 30 of about 145,939 (263)

Engineering of Streptomyces lividans for heterologous expression of secondary metabolite gene clusters

Microbial Cell Factories, 2020
Background Heterologous expression of secondary metabolite gene clusters is used to achieve increased production of desired compounds, activate cryptic gene clusters, manipulate clusters from genetically unamenable strains, obtain natural products from ...
Yousra Ahmed, Yuriy Rebets, Marta Rodríguez Estévez, Josef Zapp, Maksym Myronovskyi, Andriy Luzhetskyy   +5 more
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Robust expression and secretion of Xylanase1 in Chlamydomonas reinhardtii by fusion to a selection gene and processing with the FMDV 2A peptide. [PDF]

PLoS ONE, 2012
Microalgae have recently received attention as a potential low-cost host for the production of recombinant proteins and novel metabolites. However, a major obstacle to the development of algae as an industrial platform has been the poor expression of ...
Beth A Rasala, Philip A Lee, Zhouxin Shen, Steven P Briggs, Michael Mendez, Stephen P Mayfield   +5 more
doaj   +1 more source

Exploration of the Strategy for Improving the Expression of Heterologous Sweet Protein Monellin in Aspergillus niger

Journal of Fungi, 2023
Aspergillus niger is a primary cell factory for food-grade protein (enzyme) production due to its strong protein secretion capacity and unique safety characteristics. The bottleneck issue for the current A.
Ke Li, Junwei Zheng, Leyi Yu, Bin Wang, Li Pan   +4 more
doaj   +1 more source

18-Hydroxydolabella-3,7-diene synthase – a diterpene synthase from Chitinophaga pinensis

Beilstein Journal of Organic Chemistry, 2017
The product obtained in vitro from a diterpene synthase encoded in the genome of the bacterium Chitinophaga pinensis, an enzyme previously reported to have germacrene A synthase activity during heterologous expression in Escherichia coli, was identified ...
Jeroen S. Dickschat, Jan Rinkel, Patrick Rabe, Arman Beyraghdar Kashkooli, Harro J. Bouwmeester   +4 more
doaj   +1 more source

Recombinant production of bacterial toxins and their derivatives in the methylotrophic yeast Pichia pastoris

Microbial Cell Factories, 2005
The methylotrophic yeast Pichia pastoris is a popular heterologous expression host for the recombinant production of a variety of prokaryotic and eukaryotic proteins. The rapid emergence of P.
Gurkan Cemal, Ellar David J
doaj   +1 more source

Production of Recombinant Biopharmaceuticals in Chlamydomonas reinhardtii

International Journal of Plant Biology, 2022
This review aimed to present Chlamydomonas reinhardtii as an alternative for heterologous protein production, especially for biopharmaceuticals, and its general characteristics when compared with other expression systems. The need to produce heterologous
Cesar Andres Diaz Arias, Caroline Frere Martiniuc de Oliveira, João Vitor Dutra Molino, Livia Seno Ferreira-Camargo, Marcelo Chuei Matsudo, João Carlos Monteiro de Carvalho   +5 more
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Broad-Host-Range Expression Reveals Native and Host Regulatory Elements That Influence Heterologous Antibiotic Production in Gram-Negative Bacteria

mBio, 2017
Heterologous expression has become a powerful tool for studying microbial biosynthetic gene clusters (BGCs). Here, we extend the transformation-associated recombination cloning and heterologous expression platform for microbial BGCs to include Gram ...
Jia Jia Zhang, Xiaoyu Tang, Michelle Zhang, Darlene Nguyen, Bradley S. Moore   +4 more
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Altered fatty acid composition of Nicotiana benthamiana and Nicotiana excelsior leaves under transient overexpression of the cyanobacterial desC gene

Biologia Plantarum, 2020
Transient heterologous gene expression in two model plant species, Nicotiana benthamiana and N. excelsior, has been used to study the localization of the heterologous Δ9 acyl-lipid desaturase (Δ9 desaturase) of Synechococcus vulcanus in different cell ...
M. BERESTOVOY, O.S. PAVLENKO, A.A. TYURIN, E.N. GORSHKOVA, I.V. GOLDENKOVA-PAVLOVA   +4 more
doaj   +1 more source

Heterologous expression facilitates the discovery and characterization of marine microbial natural products

Engineering Microbiology
Microbial natural products and their derivatives have been developed as a considerable part of clinical drugs and agricultural chemicals. Marine microbial natural products exhibit diverse chemical structures and bioactivities with substantial potential ...
Shuang Zhao, Ruiying Feng, Yuan Gu, Liyuan Han, Xiaomei Cong, Yang Liu, Shuo Liu, Qiyao Shen, Liujie Huo, Fu Yan   +9 more
doaj   +1 more source

Current state of molecular and metabolic strategies for the improvement of L-asparaginase expression in heterologous systems

Frontiers in Pharmacology, 2023
Heterologous expression of L-asparaginase (L-ASNase) has become an important area of research due to its clinical and food industry applications. This review provides a comprehensive overview of the molecular and metabolic strategies that can be used to ...
Nicolás Lefin, Javiera Miranda, Jorge F. Beltrán, Lisandra Herrera Belén, Brian Effer, Adalberto Pessoa, Jorge G. Farias, Mauricio Zamorano   +7 more
doaj   +1 more source