Results 91 to 100 of about 700,500 (193)

Complete protection against P. berghei malaria upon heterologous prime/boost immunization against circumsporozoite protein employing Salmonella type III secretion system and Bordetella adenylate cyclase toxoid [PDF]

, 2008
Sterile immunity against malaria can be achieved by the induction of IFNgamma-producing CD8(+) T cells that target infected hepatocytes presenting epitopes of the circumsporozoite protein (CSP). In the present study we evaluate the protective efficacy of
Sebo, Peter, Kamanova, Jana, Jacobs, Thomas, Tartz, Susanne, Fleischer, Bernhard, Rüssmann, Holger, Heussler, Volker, Sturm, Angelika   +7 more
core   +1 more source

Biocatalysts for Peptide Geranylation With Linear and Cyclic Prenyl Groups

Angewandte Chemie International Edition, EarlyView.
A combination of genome mining, synthetic biology, and biocatalytic assays revealed enzymes that install linear and cyclized prenyl moieties on ribosomally or chemically synthesized peptides. The promiscuity of both enzymes, while being specific for modification of C‐terminal tryptophans, enables enzymatic access to peptide terpenoid hybrids.
Sarolt Magyari, Leon F. Schwarz, Luca Schlautmann, Kai Schließmann, Christopher Slotman, Nina von Felten, Claus‐Michael Lehr, Rolf Müller, Alexander F. Kiefer, Jörn Piel, Florian Hubrich   +10 more
wiley   +1 more source

Genetic heterologous prime–boost vaccination strategies for improved systemic and mucosal immunity

Expert Review of Vaccines, 2009
In this article, we discuss the advantages and progress made with heterologous prime-boost vaccination strategies. Although the consecutive use of DNA and recombinant viral vectors induce greatly enhanced and sustained levels of both cell-mediated and humoral immunity in preclinical models, the results have not yet been translated to clinical use ...
Ramshaw, Ian, Ranasinghe, Charani
openaire   +3 more sources

Isolation and Characterization of Random Citrate Synthase Variants That Increase Acetate Generation by Escherichia coli

Biotechnology and Bioengineering, EarlyView.
ABSTRACT A method to select Escherichia coli citrate synthase variants having reduced activity was developed based initially on the increased generation of acid, observed visually by prolonged color change in the pH indicator bromocresol purple. Five hundred fifty‐two potential variants were isolated and each were cultured on glucose as sole carbon ...
Jeffrey K. Dodelin, Ronnie L. Fulton, Abigail E. Rose, Mark A. Eiteman   +3 more
wiley   +1 more source

Comparison of vaccine efficacy elicited with heterologous DNA-VLP prime-boost versus homologous DNA-DNA, VLP-VLP prime-boost strategies in mice following 1000 MLD₅₀ of homologous (A/Shenzhen/406H/06, clade 2.3.4) and heterologous (A/Cambodia/P0322095/05, clade 1) H5N1 virus challenge.

, 2013
A) Percentage of original body weight in individual mice after the 1,000 MLD50 of homologous H5N1 virus challenge. Black color: PBS control group; red color: heterologous DNA-VLP prime-boost group; pink color: homologous VLP-VLP prime-boost group; blue ...
Fan Zhou (196667), Philippe Buchy (106901), Cheguo Tsai (354200), Paul Zhou (354201), Ramona Alikiiteaga Gutiérrez (193968), Vincent Deubel (204926), Heng Ding (354199)   +6 more
core   +1 more source

Exosome‐mimetic vesicle‐encapsulated multi‐responsive antibacterial hydrogel core‐shell microneedle patch promotes scarless healing of infected wounds

BMEMat, EarlyView.
The core‐shell microneedle patch VP@FNV‐CuO2‐MN was fabricated by encapsulating n‐CuO2 in a moderately crosslinked PVA/CMCS/Borax4 hydrogel shell and VP‐loaded fibroblast membrane nanovesicles (VP@FNV) in a highly crosslinked PVA/CMCS/Borax6 hydrogel core. Upon application to an infected wound, the acidic microenvironment triggers Fenton‐like reactions
Ziwei Jiang, Huihui Zhang, Lianglong Chen, Zhangfeifan Yang, Sai Cao, Yufang He, Xiaoyang Liu, Siew Yee Wong, Xu Li, Hai Zhou, Ziyan Li, Zijun Zheng, Lei Yang   +12 more
wiley   +1 more source

Heterologous Prime-Boost Vaccination with the LACK Antigen Protects against Murine Visceral Leishmaniasis [PDF]

Infection and Immunity, 2005
ABSTRACTThis study reports the efficacy of a heterologous prime-boost vaccination using DNA and vaccinia viruses (Western Reserve [WR] virus and modified [attenuated] vaccinia virus Ankara [MVA]) expressing the LACK antigen (Leishmaniahomologue of receptors for activated C kinase) and an intradermal murine infection model employingLeishmania infantum ...
Blaise, Dondji, Eva, Pérez-Jimenez, Karen, Goldsmith-Pestana, Mariano, Esteban, Diane, McMahon-Pratt   +4 more
openaire   +2 more sources

Comparison of vaccine efficacy elicited with heterologous DNA-VLP prime-boost versus homologous DNA-DNA, VLP-VLP prime-boost strategies in mice following 10 MLD₅₀ of homologous (A/Shenzhen/406H/06, clade 2.3.4) and heterologous (A/Cambodia/P0322095/05, clade 1) H5N1 virus challenge.

, 2013
A) Percentage of original body weight in individual mice after challenged with 10 MLD50 of homologous (A/Shenzhen/406H/06, clade 2.3.4) H5N1 virus. Black color: PBS control group; red color: heterologous DNA-VLP prime-boost group; pink color: homologous ...
Fan Zhou (196667), Philippe Buchy (106901), Cheguo Tsai (354200), Paul Zhou (354201), Ramona Alikiiteaga Gutiérrez (193968), Vincent Deubel (204926), Heng Ding (354199)   +6 more
core   +1 more source

Bridging the translational gap in clinical nanomedicine: From rational design to clinical reality

BMEMat, EarlyView.
Nanotechnology offers a multi‐faceted approach to modern healthcare, encompassing high‐sensitivity diagnostic imaging, innovative vaccine delivery, and targeted therapeutics. This review explores how these nanomedicine applications address critical challenges in cancer recurrence and the treatment of neurologic and respiratory diseases to combat rising
Ahmed H. Ghonaim, Marwa Ahmed El‐Naka, Wedad M. Nageeb, Atta Ullah Khan, Noura M. Seleem, Aliaa Abdelghafar, Doaa Zamel, Yang Liu, Qigai He, Wentao Li   +9 more
wiley   +1 more source

Potent immunogenicity of DNA vaccines encoding Plasmodium vivax transmission-blocking vaccine candidates Pvs25 and Pvs28 - Evaluation of homologous and heterologous antigen-delivery prime-boost strategy

, 2004
Transmission-blocking vaccines target the sexual stages of the malaria parasite and prevent further development within the mosquito vector halting the transmission of the parasite.
Kongkasuriyachai, Darin, Sattabongkot, Jetsumon, Collins, William E., Stowers, Anthony, Torii, Motomi, Kumar, Nirbhay, Bartels-Andrews, Lucy, Tsuboi, Takafumi, Sullivan, Joann   +8 more
core   +1 more source