Results 171 to 180 of about 29,136 (254)

A Comparative Biochemical Study of Oleate Hydratases

ChemBioChem, Volume 27, Issue 15, 14 August 2026.
Four oleate hydratases (Ohys) from diverse homologous families (HFams) were characterized side‐by‐side in terms of expression levels, oligomeric state, flavin adenine dinucleotide (FAD) affinity, thermostability, and activity. A general favorable effect of the reduction of FAD to FADH2 for Ohy activity was demonstrated, with (R)‐10‐hydroxystearic acid (
Maxim van Delft, Alejandro Gran‐Scheuch, Ulf Hanefeld, Peter‐Leon Hagedoorn   +3 more
wiley   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, Volume 16, Issue 8, Page 1452-1458, August 2026.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Streptavidins Coordinate Biotin Sequestration and Self‐Resistance Within a Biotin‐Pathway Antibiotic Network

Advanced Science, Volume 13, Issue 46, 17 August 2026.
A conserved genomic region between two streptavidin genes in Streptomyces packages biosynthetic gene clusters for diverse biotin‐pathway antibiotics: acidomycin, stravidin, the new non‐proteinogenic amino acid ANDA, and the new BioA inhibitor α‐methyl‐KAPA.
Sumire Kurosawa, Jingjun Mo, Xiaotong Diao, Feng Xie, Tingting Wang, Haowen Zhao, Dalei Wu, Rolf Müller, Chengzhang Fu   +8 more
wiley   +1 more source

Comparison of Receptor‐Mediated Endocytosis and Its Application to Enhance DNA Transfection by TFAMoplex

Advanced NanoBiomed Research, Volume 6, Issue 8, August 2026.
We developed an assay to distinguish cellular binding from internalization. Compatible with microscopy and high‐throughput screening, the method identifies ligand‐mediated uptake. Applying top candidates to a protein‐based DNA carrier enhanced transfection efficiency, providing a rational strategy to improve non‐viral gene delivery systems.
David Scherer, Steffen Honrath, Jonas Kurmann, Alexander Klipp, Finn Brigger, Jean‐Christophe Leroux   +5 more
wiley   +1 more source

Comparative immunogenicity and neutralizing antibody responses post heterologous vaccination with CoronaVac (Sinovac) and Vaxzevria (AstraZeneca) in HIV-infected patients with varying CD4+ T lymphocyte counts. [PDF]

Hum Vaccin Immunother
Chittrakarn S, Siripaitoon P, Chusri S, Kanchanasuwan S, Charoenmak B, Hortiwakul T, Kantikit P, Kositpantawong N.   +7 more
europepmc   +1 more source

Endochitinase Overexpression in Transgenic Tomato Plants Improves Resistance to Phytopathogens

Biotechnology Journal, Volume 21, Issue 8, August 2026.
Graphical Abstract and Lay Summary Transgenic tomato plants overexpressing an endochitinase gene showed confirmed transgene expression and enhanced resistance. Upon plants inoculations with Xanthomonas euvesicatoria pv. perforans and Sclerotinia sclerotiorum, disease severity was significantly reduced compared to wild‐type plants, demonstrating broad ...
Lucas José de Sousa, Ana Carolina Mendes Bezerra, Ivonaldo Reis Santos, Ana Clara Muniz Martins Vilela, Osmundo Brilhante de Oliveira Neto, Glaucia Barbosa Cabral, Alice Maria Quezado‐Duval, Pedro Ricardo Vieira Hamann, Eliane Ferreira Noronha, Luiz Eduardo Bassay Blum, Angela Mehta   +10 more
wiley   +1 more source

Corrigendum: Heterologous vaccination with inactivated vaccine and mRNA vaccine augments antibodies against both spike and nucleocapsid proteins of SARS-CoV-2: a local study in Macao. [PDF]

Front Immunol
Ng HM, Lei CL, Fu S, Li E, Leong SI, Nip CI, Choi NM, Lai KS, Tang XJ, Lei CL, Xu RH.   +10 more
europepmc   +1 more source

The power of many: when genetics met yeasts and high‐throughput

Biological Reviews, Volume 101, Issue 4, Page 1662-1682, August 2026.
ABSTRACT In recent years, complex technological capabilities have evolved, driven by the need to solve complex and integrative biological questions through global analyses. New equipment allows the scaling up and automation of processes which previously were carried out on a very limited scale.
Víctor A. Tallada, Víctor Carranco
wiley   +1 more source

Deep Mutational Scanning for the Study and Engineering of Protein Assemblies

Chemistry–Methods, Volume 6, Issue 8, August 2026.
This review explores how deep mutational scanning (DMS) can be used to improve understanding and accelerate engineering of protein assemblies, from natural fibers to engineered nanocages. We discuss various types of protein assemblies and their characterization, provide an introduction to the DMS technique, and then highlight examples in which DMS has ...
Jenna B. Wolfanger, Shoili Banerjee, Carolyn E. Mills   +2 more
wiley   +1 more source

Humoral and cellular responses to SARS‐CoV‐2 variants after ancestral COVID‐19 vaccines in people with HIV and lung transplant recipients

Clinical &Translational Immunology, Volume 15, Issue 8, 2026.
This study compares the humoral and cellular immunogenicity of Dose 3 of Ancestral SARS‐CoV‐2 vaccine in participants with HIV and/or following lung transplantation with healthy controls. We highlight differences along a spectrum of immunocompromise, and the value of SARS‐CoV‐2 vaccination, even in the absence of detectable neutralisation responses ...
David WJ Griffin, Paul A Gill, Irene Boo, Shir Sun, Raffi Gugasyan, Alina Wang, Scott J Bornheimer, Stuart Turville, Anupriya Aggarwal, Greg I Snell, Glen P Westall, Emily SJ Edwards, Anna Coldham, Menno C van Zelm, Heidi E Drummer, James H McMahon   +15 more
wiley   +1 more source