Results 21 to 30 of about 4,193 (115)
The Hfq regulon of Neisseria meningitidis
FEBS Open Bio, 2017 The conserved RNA‐binding protein, Hfq, has multiple regulatory roles within the prokaryotic cell, including promoting stable duplex formation between small RNAs and mRNAs, and thus hfq deletion mutants have pleiotropic phenotypes. Previous proteome and transcriptome studies of Neisseria meningitidis have generated limited insight into differential ...
Robert A. G. Huis in ‘t Veld +4 more
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Hfq variant with altered RNA binding functions [PDF]
Nucleic Acids Research, 2006 The interaction between Hfq and RNA is central to multiple regulatory processes. Using site-directed mutagenesis, we have found a missense mutation in Hfq (V43R) which strongly affects2 the RNA binding capacity of the Hfq protein and its ability to stimulate poly(A) tail elongation by poly(A)-polymerase in vitro.
Ziolkowska, K. +6 more
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Hfq proximity and orientation controls RNA annealing [PDF]
Nucleic Acids Research, 2012 Regulation of bacterial gene networks by small non-coding RNAs (sRNAs) requires base pairing with messenger RNA (mRNA) targets, which is facilitated by Hfq protein. Hfq is recruited to sRNAs and mRNAs through U-rich- and A-rich-binding sites, respectively, but their distance from the sRNA-mRNA complementary region varies widely among different genes ...
Panja, Subrata, Woodson, Sarah A.
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E. coli DNA associated with isolated Hfq interacts with Hfq's distal surface and C-terminal domain [PDF]
Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, 2010 The RNA-binding protein Hfq has been studied extensively for its function as a modulator of gene expression at the post-transcriptional level. While most Hfq studies have focused on the protein's interaction with sRNAs and mRNAs, Hfq binding to DNA has been observed but is less explored.
Taylor B, Updegrove +4 more
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Hfq affects mRNA levels independently of degradation [PDF]
BMC Molecular Biology, 2010 Abstract Background The bacterial Lsm protein, Hfq, is an RNA chaperone involved in many reactions related to RNA metabolism, such as replication and stability, control of small RNA activity and polyadenylation. Despite this wide spectrum of known functions, the global role of Hfq is almost certainly undervalued; its ...
Le Derout, Jacques +3 more
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The Hfq chaperone helps the ribosome mature [PDF]
The EMBO Journal, 2018 Ribosome biogenesis is a complex process that is facilitated by a large number of assembly factors. In this issue, Andrade et al (2018) provide evidence that a widely conserved RNA chaperone, Hfq, acts as a ribosomal assembly factor in bacteria. Hfq is known to support regulation of stress response genes by small RNAs.
Indra Mani Sharma +2 more
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Prediction of Hfq in actinobacteria [PDF]
, 2015 AbstractHfq is the bacterial orthologue of the eukaryotic (L)Sm family of proteins found across all domains of life and potentially an ancient protein, but it has not been found in all phyletic lines. A careful search successfully identified a distant hfq orthologue in the cyanobacteria leaving the actinobacteria as the major phylum with no known hfq ...
Bora, Nagamani +2 more
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Central role for RNase YbeY in Hfq-dependent and Hfq-independent small-RNA regulation in bacteria [PDF]
BMC Genomics, 2014 Conceptual parallels exist between bacterial and eukaryotic small-RNA (sRNA) pathways, yet relatively little is known about which protein may recognize and recruit bacterial sRNAs to interact with targets. In eukaryotes, Argonaute (AGO) proteins discharge such functions.
Pandey, Shree P. +5 more
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Identification of the Hfq-binding site on DsrA RNA: Hfq binds without altering DsrA secondary structure [PDF]
RNA, 2003 DsrA RNA regulates the translation of two global regulatory proteins in Escherichia coli. DsrA activates the translation of RpoS while repressing the translation of H-NS. The RNA-binding protein Hfq is necessary for DsrA to function in vivo. Although Hfq binds to DsrA in vitro, the role of Hfq in DsrA-mediated regulation is not known.
Cristin C, Brescia +3 more
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Defining a role for Hfq in Gram-positive bacteria: evidence for Hfq-dependent antisense regulation in Listeria monocytogenes [PDF]
Nucleic Acids Research, 2009 Small trans-encoded RNAs (sRNAs) modulate the translation and decay of mRNAs in bacteria. In Gram-negative species, antisense regulation by trans-encoded sRNAs relies on the Sm-like protein Hfq. In contrast to this, Hfq is dispensable for sRNA-mediated riboregulation in the Gram-positive species studied thus far.
Nielsen, Jesper Sejrup +6 more
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