Unnatural Amino Acid and Emerging Chemistry Approaches to Map RNA–Protein Interactions
Angewandte Chemie, EarlyView.This review highlights emerging chemistries for mapping RNA–protein interactions, including genetically encoded unnatural amino acids, novel photocrosslinkers, and non‐photoactivatable crosslinking systems. We compare their mechanisms, reactivity and applications, outlining how these next‐generation tools enable higher‐resolution, site‐specific ...
Eryn Lundrigan +3 more
wiley +2 more sources
Regulation of virulence in Francisella tularensis by small non-coding RNAs [PDF]
, 2011 Using a cDNA cloning and sequencing approach we have shown that Francisella tularensis expresses homologues of several small RNAs (sRNAs) that are well-conserved among diverse bacteria.
Alain Charbit +6 more
core +2 more sources
The Pseudomonas aeruginosa CrcZ RNA interferes with Hfq-mediated riboregulation.
PLoS ONE, 2017 The RNA chaperone Hfq regulates virulence and metabolism in the opportunistic pathogen Pseudomonas aeruginosa. During carbon catabolite repression (CCR) Hfq together with the catabolite repression control protein Crc can act as a translational repressor ...
Elisabeth Sonnleitner +2 more
doaj +1 more source
The Pleiotropic Phenotypes Caused by an hfq Null Mutation in Vibrio harveyi
Microorganisms, 2023 Hfq is a global regulator and can be involved in multiple cellular processes by assisting small regulatory RNAs (sRNAs) to target mRNAs. To gain insight into the virulence regulation of Hfq in Vibrio harveyi, the hfq null mutant, ∆hfq, was constructed in
Yiqin Deng +5 more
doaj +1 more source
Identifying and characterizing Hfq–RNA interactions [PDF]
Methods, 2013 To regulate stress responses and virulence, bacteria use small regulatory RNAs (sRNAs). These RNAs can up or down regulate target mRNAs through base pairing by influencing ribosomal access and RNA decay. A large class of these sRNAs, called trans-encoded sRNAs, requires the RNA binding protein Hfq to facilitate base pairing between the regulatory RNA ...
M A, Faner, A L, Feig
openaire +2 more sources
Apomorphine Targets the Pleiotropic Bacterial Regulator Hfq [PDF]
Antibiotics, 2021 Hfq is a bacterial regulator with key roles in gene expression. The protein notably regulates translation efficiency and RNA decay in Gram-negative bacteria, thanks to its binding to small regulatory noncoding RNAs. This property is of primary importance for bacterial adaptation and survival in hosts.
Florian Turbant +6 more
openaire +3 more sources
Identification of small RNAs abundant in Burkholderia cenocepacia biofilms reveal putative regulators with a potential role in carbon and iron metabolism [PDF]
, 2017 Small RNAs play a regulatory role in many central metabolic processes of bacteria, as well as in developmental processes such as biofilm formation. Small RNAs of Burkholderia cenocepacia, an opportunistic pathogenic beta-proteobacterium, are to date not ...
Coenye, Tom +2 more
core +3 more sources
The Irr and RirA proteins participate in a complex regulatory circuit and act in concert to modulate bacterioferritin expression in Ensifer meliloti 1021 [PDF]
, 2017 In this work we found that the bfr gene of the rhizobial species Ensifer meliloti, encoding a bacterioferritin iron storage protein, is involved in iron homeostasis and the oxidative stress response. This gene is located downstream of and overlapping the
Amarelle, Vanesa +4 more
core +1 more source
The hyperfine structure of highly charged $^{238}_{92}$U ions with rotationally excited nuclei [PDF]
, 1999 The hyperfine structure (hfs) of electron levels of $^{238}_{92}$U ions with the nucleus excited in the low-lying rotational $2^+$ state with an energy $E_{2^+} = 44.91$ keV is investigated.
Labzowsky, L. N. +4 more
core +2 more sources
Central role for RNase YbeY in Hfq-dependent and Hfq-independent small-RNA regulation in bacteria [PDF]
BMC Genomics, 2014 Conceptual parallels exist between bacterial and eukaryotic small-RNA (sRNA) pathways, yet relatively little is known about which protein may recognize and recruit bacterial sRNAs to interact with targets. In eukaryotes, Argonaute (AGO) proteins discharge such functions.
Pandey, Shree P. +5 more
openaire +4 more sources