Defining a role for Hfq in Gram-positive bacteria: evidence for Hfq-dependent antisense regulation in Listeria monocytogenes [PDF]
Nucleic Acids Research, 2009 Small trans-encoded RNAs (sRNAs) modulate the translation and decay of mRNAs in bacteria. In Gram-negative species, antisense regulation by trans-encoded sRNAs relies on the Sm-like protein Hfq. In contrast to this, Hfq is dispensable for sRNA-mediated riboregulation in the Gram-positive species studied thus far.
Nielsen, Jesper Sejrup +6 more
openaire +4 more sources
Non-Coding RNA, 2021 Many RNA-RNA interactions depend on molecular chaperones to form and remain stable in living cells. A prime example is the RNA chaperone Hfq, which is a critical effector involved in regulatory interactions between small RNAs (sRNAs) and cognate target ...
David Lalaouna +8 more
doaj +1 more source
Structural and biochemical studies on ATP binding and hydrolysis by the Escherichia coli RNA chaperone Hfq. [PDF]
PLoS ONE, 2012 In Escherichia coli the RNA chaperone Hfq is involved in riboregulation by assisting base-pairing between small regulatory RNAs (sRNAs) and mRNA targets. Several structural and biochemical studies revealed RNA binding sites on either surface of the donut
Hermann Hämmerle +6 more
doaj +1 more source
Springback analysis of AA5754 after hot stamping: experiments and FE modelling [PDF]
, 2016 In this paper, the springback of the aluminium alloy AA5754 under hot stamping conditions was characterised under stretch and pure bending conditions. It was found that elevated temperature stamping was beneficial for springback reduction, particularly ...
Ailing Wang +7 more
core +1 more source
Isofunctional but Structurally Different Methyltransferases for Dithiolopyrrolone Diversification
Angewandte Chemie, Volume 136, Issue 49, December 2, 2024.Two for one job: DtpM and XrdM are two bacterial enzymes that have different structures but still catalyze the same reaction: the amide N‐methylation of dithiolopyrrolones (DTPs). Ligand binding to both enzymes was studied using X‐ray crystallography, modeling, mutagenesis and activity assays.
Li Su +13 more
wiley +2 more sources
The Escherichia coli Hfq protein: an unattended DNA-transactions regulator
Frontiers in Molecular Biosciences, 2016 The Hfq protein was discovered in Escherichia coli as a host factor for bacteriophage Qβ RNA replication. Subsequent studies indicated that Hfq is a pleiotropic regulator of bacterial gene expression.
Grzegorz M Cech +6 more
doaj +1 more source
Monopole Floer homology for rational homology 3-spheres
, 2010 We give a new construction of monopole Floer homology for spin-c rational homology 3-spheres. As applications we define two invariants of certain smooth compact 4-manifolds with b_1=1 and b^+=0.Comment: 60 pages, to appear in Duke J. Math.
Froyshov, Kim A.
core +1 more source
Hfq variant with altered RNA binding functions [PDF]
Nucleic Acids Research, 2006 The interaction between Hfq and RNA is central to multiple regulatory processes. Using site-directed mutagenesis, we have found a missense mutation in Hfq (V43R) which strongly affects2 the RNA binding capacity of the Hfq protein and its ability to stimulate poly(A) tail elongation by poly(A)-polymerase in vitro.
Olivier Pellegrini +6 more
openaire +4 more sources
The Irr and RirA proteins participate in a complex regulatory circuit and act in concert to modulate bacterioferritin expression in Ensifer meliloti 1021 [PDF]
, 2017 In this work we found that the bfr gene of the rhizobial species Ensifer meliloti, encoding a bacterioferritin iron storage protein, is involved in iron homeostasis and the oxidative stress response. This gene is located downstream of and overlapping the
Amarelle, Vanesa +4 more
core +1 more source
E. coli DNA associated with isolated Hfq interacts with Hfq's distal surface and C-terminal domain [PDF]
Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, 2010 The RNA-binding protein Hfq has been studied extensively for its function as a modulator of gene expression at the post-transcriptional level. While most Hfq studies have focused on the protein's interaction with sRNAs and mRNAs, Hfq binding to DNA has been observed but is less explored.
Taylor B. Updegrove +4 more
openaire +3 more sources