Results 51 to 60 of about 6,261 (210)

Central role for RNase YbeY in Hfq-dependent and Hfq-independent small-RNA regulation in bacteria [PDF]

BMC Genomics, 2014
Conceptual parallels exist between bacterial and eukaryotic small-RNA (sRNA) pathways, yet relatively little is known about which protein may recognize and recruit bacterial sRNAs to interact with targets. In eukaryotes, Argonaute (AGO) proteins discharge such functions.
Pandey, Shree P., Winkler, Jonathan A., Li, Hu, Camacho, Diogo M., Collins, James J., Walker, Graham C.   +5 more
openaire   +4 more sources

The Pleiotropic Phenotypes Caused by an hfq Null Mutation in Vibrio harveyi

Microorganisms, 2023
Hfq is a global regulator and can be involved in multiple cellular processes by assisting small regulatory RNAs (sRNAs) to target mRNAs. To gain insight into the virulence regulation of Hfq in Vibrio harveyi, the hfq null mutant, ∆hfq, was constructed in
Yiqin Deng, Shujun Zang, Ziyang Lin, Liwen Xu, Changhong Cheng, Juan Feng   +5 more
doaj   +1 more source

Identifying and characterizing Hfq–RNA interactions [PDF]

Methods, 2013
To regulate stress responses and virulence, bacteria use small regulatory RNAs (sRNAs). These RNAs can up or down regulate target mRNAs through base pairing by influencing ribosomal access and RNA decay. A large class of these sRNAs, called trans-encoded sRNAs, requires the RNA binding protein Hfq to facilitate base pairing between the regulatory RNA ...
M A, Faner, A L, Feig
openaire   +2 more sources

Hfq C-terminal region forms a β-rich amyloid-like motif without perturbing the N-terminal Sm-like structure

Communications Biology, 2023
Hfq is a pleitropic actor that serves as stress response and virulence factor in the bacterial cell. To execute its multiple functions, Hfq assembles into symmetric torus-shaped hexamers.
Mélanie Berbon, Denis Martinez, Estelle Morvan, Axelle Grélard, Brice Kauffmann, Jehan Waeytens, Frank Wien, Véronique Arluison, Birgit Habenstein   +8 more
doaj   +1 more source

Unnatural Amino Acid and Emerging Chemistry Approaches to Map RNA–Protein Interactions

Angewandte Chemie, Volume 138, Issue 6, 2 February 2026.
This review highlights emerging chemistries for mapping RNA–protein interactions, including genetically encoded unnatural amino acids, novel photocrosslinkers, and non‐photoactivatable crosslinking systems. We compare their mechanisms, reactivity and applications, outlining how these next‐generation tools enable higher‐resolution, site‐specific ...
Eryn Lundrigan, Parrish Evers, Elexa Scott, John Paul Pezacki   +3 more
wiley   +2 more sources

Structural insights into the dynamics and function of the C-terminus of the E. coli RNA chaperone Hfq

, 2011
The hexameric Escherichia coli RNA chaperone Hfq (Hfq(Ec)) is involved in riboregulation of target mRNAs by small trans-encoded RNAs. Hfq proteins of different bacteria comprise an evolutionarily conserved core, whereas the C-terminus is variable in ...
Beich-Frandsen, Mads (Department of Structural and Computational Biology, Centre for Molecular Biology, University of Vienna), Kontaxis, Georg (Department of Structural and Computational Biology, Centre for Molecular Biology, University of Vienna), Blasi, U., Rajkowitsch, L., Miles, Andrew (Birkbeck College), Sjöblom, Björn (Department of Structural and Computational Biology, Centre for Molecular Biology, University of Vienna), Miles, Andrew J., Svergun, D.I., Kloiber, K., Kristina Djinović-Carugo, Vecerek, B., Karin Kloiber, Konarev, P.V., Lukas Rajkowitsch, Hämmerle, Hermann Konrad (Department of Biochemistry and Cell Biology, Centre for Molecular Biology, University of Vienna), Georg Kontaxis, Bläsi, Udo (Department of Microbiology, Immunobiology and Genetics, Centre for Molecular Biology, University of Vienna), Djinovic-Carugo, K., Robert Konrat, Branislav Večerek, Konarev, Peter V. (EMBL Hamburg), Andrew J. Miles, Kloiber, Karin (Department of Structural and Computational Biology, Centre for Molecular Biology, University of Vienna), Rajkowitsch, Lukas (Department of Biochemistry and Cell Biology, Centre for Molecular Biology, University of Vienna), Udo Bläsi, Wallace, Bonnie A., Svergun, D. I., Petr V. Konarev, Svergun, Dimitri I. (EMBL Hamburg), Björn Sjöblom, Hammerle, H., Mads Beich-Frandsen, Wallace, B. A., Sjoblom, B., Miles, A. J., Konrat, Robert (Department of Structural and Computational Biology, Centre for Molecular Biology, University of Vienna), Vecerek, Branislav (Department of Microbiology, Immunobiology and Genetics, Centre for Molecular Biology, University of Vienna), Dimitri I. Svergun, B. A. Wallace, Beich Frandsen, M., Djinovic Carugo, K., Konarev, P. V., Beich-Frandsen, M., Konrat, R., Djinovic-Carugo, Kristina (Department of Structural and Computational Biology, Centre for Molecular Biology, University of Vienna), Hermann Hämmerle, Kontaxis, G.   +46 more
core   +1 more source

Interaction of the RNA chaperone Hfq with mRNAs: direct and indirect roles of Hfq in iron metabolism of Escherichia coli [PDF]

Molecular Microbiology, 2003
SummaryThe Escherichia coli Sm‐like host factor I (Hfq) is thought to play direct and indirect roles in post‐transcriptional regulation by targeting small regulatory RNAs and mRNAs. In this study, we have used proteomics to identify new mRNA targets of Hfq.
Vecerek, Branislav, Moll, Isabella, Afonyushkin, Taras, Kaberdin, Vladimir, Bläsi, Udo   +4 more
openaire   +3 more sources

E. coli DNA associated with isolated Hfq interacts with Hfq's distal surface and C-terminal domain [PDF]

Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, 2010
The RNA-binding protein Hfq has been studied extensively for its function as a modulator of gene expression at the post-transcriptional level. While most Hfq studies have focused on the protein's interaction with sRNAs and mRNAs, Hfq binding to DNA has been observed but is less explored.
Taylor B, Updegrove, John J, Correia, Roberto, Galletto, Wlodzimierz, Bujalowski, Roger M, Wartell   +4 more
openaire   +2 more sources

Uncovering a previously unknown function of polyphosphate in polyadenylated RNA‐induced amyloidogenesis of Hfq

The FEBS Journal, EarlyView.
Polyphosphate interaction with Hfq and RNA drives phase separation and amyloid self‐assembly, uncovering a previously unrecognized role for polyP in bacterial stress responses. Our findings reveal new insights into the molecular mechanisms of RNA‐mediated phase separation, highlighting the role of polyP as a key modulator of this process. Polyphosphate
Kevin Mosca, Florian Turbant, Sambhasan Banerjee, Frank Wien, Richard R. Sinden, Véronique Arluison   +5 more
wiley   +1 more source

Sm‐like protein Hfq: Location of the ATP‐binding site and the effect of ATP on Hfq–RNA complexes [PDF]

Protein Science, 2007
AbstractSm‐like proteins are ubiquitous ring‐shaped oligomers that exhibit a variety of nucleic acid‐binding activities. They have been linked functionally to various cellular events involving RNA, and it is generally believed that their activity is exerted via the passive binding of nucleic acids.
Arluison, Veronique, Mutyam, Shravan, Mura, Cameron, Marco, Sergio, Sukhodolets, Maxim   +4 more
openaire   +3 more sources