Results 81 to 90 of about 6,621 (215)

Genome-wide profiling of Hfq-bound RNAs reveals the iron-responsive small RNA RusT in Caulobacter crescentus

mBio
The alphaproteobacterium Caulobacter crescentus thrives in oligotrophic environments and is able to optimally exploit minimal resources by entertaining an intricate network of gene expression control mechanisms.
Laura N. Vogt, Gaёl Panis, Anna Schäpers, Nikolai Peschek, Michaela Huber, Kai Papenfort, Patrick H. Viollier, K. Fröhlich   +7 more
semanticscholar   +1 more source

Hfq Is a Critical Modulator of Pathogenicity of Dickeya oryzae in Rice Seeds and Potato Tubers

Microorganisms, 2022
The frequent outbreaks of soft-rot diseases caused by Dickeya oryzae have emerged as severe problems in plant production in recent years and urgently require the elucidation of the virulence mechanisms of D. oryzae.
Zurong Shi, Qingwei Wang, Shunchang Wang, Chengrun Wang, Lianjun Zhang, Zhibin Liang   +5 more
semanticscholar   +1 more source

IMACulate(DE3), an E. coli Strain for High Purity His‐Tagged Protein Purifications

Biotechnology and Bioengineering, Volume 123, Issue 8, Page 2127-2138, August 2026.
ABSTRACT Immobilised Metal Affinity Chromatography (IMAC) is widely used to purify his‐tagged recombinant proteins from Escherichia coli. However, endogenous contaminants with histidine clusters, such as GFAT and PDH E1 proteins, are often co‐purified with the target protein.
Jia Q. Truong, Dingyi Yu, Nan Hao, Chris Langendorf, Keith. E. Shearwin, Jessica K. Holien   +5 more
wiley   +1 more source

‘GGFGGQ’ repeats in Hfq of Acinetobacter baumannii are essential for nutrient utilization and virulence

Journal of Biological Chemistry
The nosocomial pathogen Acinetobacter baumannii is known for causing lung and soft tissue infections in immunocompromised hosts. Its ability to adapt to various environments through post-transcriptional gene regulation is key to its success.
Abhiroop Sett, Pulak Kumar Maiti, Kritika Garg, Arsalan Hussain, Snehlata Saini, Shivam Pandey, R. Pathania   +6 more
semanticscholar   +1 more source

RNA-RNA interactomes of ProQ and Hfq reveal overlapping and competing roles

Molecules and Cells, 2019
SUMMARY Base pairing RNAs modulate gene expression in all studied organisms. In many bacteria, the base pairing between most small regulatory RNAs (sRNAs) and their targets is mediated by the Hfq RNA chaperone.
S. Melamed, Philip P. Adams, A. Zhang, Hongen Zhang, G. Storz   +4 more
semanticscholar   +1 more source

Rewiring of Gene Expression in Pseudomonas aeruginosa During Diauxic Growth Reveals an Indirect Regulation of the MexGHI-OpmD Efflux Pump by Hfq

Frontiers in Microbiology, 2022
In Pseudomonas aeruginosa, the RNA chaperone Hfq and the catabolite repression protein Crc act in concert to regulate numerous genes during carbon catabolite repression (CCR).
Marlena Rozner, Ella Nukarinen, Michael T. Wolfinger, F. Amman, W. Weckwerth, U. Bläsi, E. Sonnleitner   +6 more
semanticscholar   +1 more source

Scotty: lattice coincidences for macromolecular crystallographic phasing

Acta Crystallographica Section D, Volume 82, Issue 7, Page 813-823, July 2026.
Scotty is a tool that leverages Niggli‐cell matches to identify similar crystallographic lattices and perform phasing with difference Fourier methods.When a macromolecular crystal lattice targeted for study is closely related to a previously studied crystal lattice, phasing the target crystal by difference (fast‐)Fourier transform (DFFT) methods is ...
Airlie J. McCoy, Lawrence C. Andrews, Herbert J. Bernstein, Randy J. Read   +3 more
wiley   +1 more source

Hfq stimulates the activity of the CCA-adding enzyme [PDF]

BMC Molecular Biology, 2007
Abstract Background The bacterial Sm-like protein Hfq is known as an important regulator involved in many reactions of RNA metabolism. A prominent function of Hfq is the stimulation of RNA polyadenylation catalyzed by E. coli poly(A) polymerase I (PAP).
Scheibe, M. ; https://orcid.org/0000-0002-2181-2214, Bonin, S., Hajnsdorf, E., Betat, H., Mörl, M.   +4 more
openaire   +3 more sources

Role of the Sinorhizobium meliloti Global Regulator Hfq in Gene Regulation and Symbiosis

Molecular Plant-Microbe Interactions, 2010
The RNA-binding protein Hfq is a global regulator which controls diverse cellular processes in bacteria. To begin understanding the role of Hfq in the Sinorhizobium meliloti–Medicago truncatula nitrogen-fixing symbiosis, we defined free-living and ...
Mengsheng Gao, Melanie J. Barnett, Sharon R. Long, Max Teplitski   +3 more
doaj   +1 more source

The RNA Chaperone Protein Hfq Regulates the Characteristic Sporulation and Insecticidal Activity of Bacillus thuringiensis

Frontiers in Microbiology, 2022
Bacillus thuringiensis (Bt) is one of the most widely used bio-insecticides at present. It can produce many virulence factors and insecticidal crystal proteins during growth and sporulation.
Zhao-Yun Yu, Yang Fu, Wei Zhang, Li Zhu, Wen Yin, S. Chou, Jin He   +6 more
semanticscholar   +1 more source