Results 91 to 100 of about 6,621 (215)

RNAs Associated With Bacterial Outer Membrane Vesicles: Structural Insights Into Surface Composition

Journal of Extracellular Vesicles, Volume 15, Issue 6, June 2026.
This work uncovers a new layer of bacterial communication. We show that RNAs, aided by the Hfq RNA‐chaperone, are not only packed inside Gram negative Outer Membrane Vesicles (OMVs) but can also be embedded in their membranes. This suggests that bacteria may use surface‐exposed RNAs to directly interact with host cells. Overall, our results broaden the
Kevin Mosca, Florian Turbant, Wafa Achouak, Frank Wien, Véronique Arluison   +4 more
wiley   +1 more source

The Amyloid Assembly of the Bacterial Hfq Is Lipid-Driven and Lipid-Specific

International Journal of Molecular Sciences
Under specific conditions, some proteins can self-assemble into fibrillar structures called amyloids. Initially, these proteins were associated with neurodegenerative diseases in eucaryotes.
Florian Turbant, Quentin Machiels, Jehan Waeytens, Frank Wien, V. Arluison   +4 more
semanticscholar   +1 more source

Pleiotropic Effects of Hfq on the Cytochrome c Content and Pyomelanin Production in Shewanella oneidensis

Applied and Environmental Microbiology, 2022
In bacteria, Hfq has been implicated in regulation of diverse biological processes posttranslationally. In S. oneidensis, Hfq affects the content of cytos c that serve as the basis of its respiratory versatility and potential application in bioenergy and
Wei Wang, Yawen Liang, Lulu Liu, Sirui Han, Shihua Wu, Haichun Gao   +5 more
semanticscholar   +1 more source

Identifying Subcellular Structure Components in Escherichia Coli by Crosslinking and SEC‐MS

PROTEOMICS, Volume 26, Issue 5, Page 27-36, May 2026.
ABSTRACT Cells are comprised of a broad spectrum of structures that compartmentalize biochemical and signaling mechanisms. These structures can be comprised of many biomolecules, but especially lipids, proteins, and nucleic acids. Techniques are limited to quantify or discover new subcellular structures.
Rachel A. Victor, Austin Lipinski, Paul R. Langlais, Jacob C. Schwartz   +3 more
wiley   +1 more source

Identification of the Hfq-binding site on DsrA RNA: Hfq binds without altering DsrA secondary structure [PDF]

RNA, 2003
DsrA RNA regulates the translation of two global regulatory proteins in Escherichia coli. DsrA activates the translation of RpoS while repressing the translation of H-NS. The RNA-binding protein Hfq is necessary for DsrA to function in vivo. Although Hfq binds to DsrA in vitro, the role of Hfq in DsrA-mediated regulation is not known.
Cristin C, Brescia, Peter J, Mikulecky, Andrew L, Feig, Darren D, Sledjeski   +3 more
openaire   +2 more sources

Hfq mediates transcriptome-wide RNA structurome reprogramming under virulence-inducing conditions in a phytopathogen.

Cell Reports
Although RNA structures play important roles in regulating gene expression, the mechanism and function of mRNA folding in plant bacterial pathogens remain elusive.
Canfeng Hua, Jiadai Huang, Yue Sun, Tingting Wang, Youyue Li, Zining Cui, Xin Deng   +6 more
semanticscholar   +1 more source

Engineering of the Small Noncoding RNA (sRNA) DsrA Together with the sRNA Chaperone Hfq Enhances the Acid Tolerance of Escherichia coli

Applied and Environmental Microbiology, 2021
Many of the traditional studies on bacterial acid tolerance generally focused on improving cell survival under extreme-pH conditions, but cell growth under less harsh acidic conditions is more relevant to industrial applications. Under normal conditions,
Zhanglin Lin, Jiahui Li, Xiaofang Yan, Jingduan Yang, Xiaofan Li, Ping Chen, Xiaofeng Yang   +6 more
semanticscholar   +1 more source

Comparative Proteomic Profiling of Clinical Klebsiella pneumoniae Strains and the Corresponding Outer Membrane Vesicles

Journal of Extracellular Biology, Volume 5, Issue 4, April 2026.
ABSTRACT Klebsiella pneumoniae (K. pneumoniae) has become a global threat due to the convergence of carbapenem resistance and hypervirulence. Outer membrane vesicles (OMVs) play important roles in bacterial pathogenicity, yet their functional coordination with strains remains unclear.
Zongping Li, Jingyuan Xi, Xinmiao Jia, Yangzhige He, Guibin Wang, Shiyu Chen, Xiaobing Chu, Qian Zhang, Ying Zhu, Wei Yu, Peiyao Jia, Xiaoyu Liu, Qiwen Yang   +12 more
wiley   +1 more source

Transcriptome-scale analysis uncovers conserved residues in the hydrophobic core of the bacterial RNA chaperone Hfq required for small regulatory RNA stability

bioRxiv
The RNA-chaperone Hfq plays crucial roles in bacterial gene expression and is a major facilitator of small regulatory RNA (sRNA) action. The toroidal molecular architecture of the Hfq hexamer contains three well characterised surfaces which allow it to ...
Josh McQuail, Miroslav Krepl, Kai Katsuya-Gaviria, Aline Tabib-Salazar, L. Burchell, Thorsten Bischler, T. Gräfenhan, P. Brear, Jiří Šponer, B. Luisi, S. Wigneshweraraj   +10 more
semanticscholar   +1 more source

Exploring the Diversity and Function of the SPFH‐Domain Containing Proteins in Pseudomonas aeruginosa

Environmental Microbiology Reports, Volume 18, Issue 2, April 2026.
We characterised nine SPFH‐domain containing proteins in Pseudomonas aeruginosa PA14 and found remarkable sequence divergence and scattered evolutionary distribution. Functional analyses showed that single or multiple deletions had no detectable impact on growth, motility, biofilm formation, stress resistance or virulence under laboratory conditions ...
Víctor Manuel García‐Maldonado, Claudia Rodríguez‐Rangel, Dimitris Georgellis, Adrián F. Alvarez   +3 more
wiley   +1 more source