Results 241 to 250 of about 56,372 (290)

Separation and purification of peptides by high-speed counter-current chromatography

Journal of Chromatography A, 1995
Preparative separations of peptides have been accomplished using high-speed counter-current chromatography. This has been made possible by the use of a particular solvent system that does not exhibit solvent carryover at high speed flow and centrifugation conditions.
M, Knight, M O, Fagarasan, K, Takahashi, A Z, Geblaoui, Y, Ma, Y, Ito   +5 more
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Rapid separation of flavonoids by analytical high-speed counter-current chromatography

Journal of Chromatography A, 1988
A commercial model of the analytical high-speed counter-current chromatography instrument was used for separation of flavonoids from a crude ethanol extract of dried fruits of sea buckthorn (Hippophae rhamnoides). Using a two-phase solvent system of chloroform-methanol-water (4:3:2), a five-fold increase in flow-rate of the mobile phase from 60 to 300 ...
T Y, Zhang, R, Xiao, Z Y, Xiao, L K, Pannell, Y, Ito   +4 more
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Separation of ivermectin components by high-speed counter-current chromatography

Journal of Chromatography A, 1996
Abstract High-speed counter-current chromatography (HSCCC) has been successfully applied to the separation of the ivermectin components. A 25-mg quantity of the sample was separated using a two-phase solvent system of n -hexane-ethyl acetate-methanol-water (19:1:10:10, v/v).
Hisao Oka, Yoshimoto Ikai, Junko Hayakawa, Ken-ichi Harada, Mokoto Suzuki, Atsuko Shimizu, Tomoko Hayashi, Kazue Takeba, Hiroyuki Nakazawa, Yoichiro Ito   +9 more
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Preparative separation of the naphthopyranone glycosides by high-speed counter-current chromatography

Journal of Chromatography A, 2001
High-speed counter-current chromatography was applied to the preparative separation and purification of naphthopyranone glycosides from a crude 70% ethanolic extract of the capitula of Paepalanthus microphyllus. The solvent system used was composed of water-ethanol-ethyl acetate-hexane (10:4:10:4, v/v).
dos Santos, L. C., Vilegas, Wagner
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Preparative separation of cacao bean procyanidins by high-speed counter-current chromatography

Journal of Chromatography B, 2016
In this work, an efficient method for preparative separation of procyanidins from raw cacao bean extract by high-speed counter-current chromatography (HSCCC) was developed. Under the optimized solvent system of n-hexane-ethyl acetate-water (1:50:50, v/v/v) with a combination of head-tail and tail-head elution modes, various procyanidins fractions with ...
Lingxi, Li, Shuting, Zhang, Yan, Cui, Yuanyuan, Li, Lanxin, Luo, Peiyu, Zhou, Baoshan, Sun   +6 more
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Resolution of gram quantities of racemates by high-speed counter-current chromatography

Journal of Chromatography A, 1995
Gram quantities of (+/-)-dinitrobenzoyl amino acids were separated by high-speed counter-current chromatography (CCC) using N-dodecanoyl-L-proline-3,5-dimethylanilide as a chiral selector (CS). Standard and pH-zone-refining CCC techniques were compared.
Y, Ma, Y, Ito, A, Foucault
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Practical approach to high-speed counter-current chromatography

Journal of Chromatography A, 1991
Abstract Counter-current chromatography (CCC0 is mainly used in the preparative separation, extraction and purification of samples. CCC does not operate with a solid stationary phase. Two immiscible liquid phases are used. One is the liquid mobile phase and the other is the liquid stationary phase.
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Separation of salidroside from Rhodiola crenulata by high-speed counter-current chromatography

Journal of Chromatography A, 2002
High-speed counter-current chromatography (HSCCC) was used to purify salidroside from an extract of Rhodiola crenulata with two steps using a two-phase solvent system composed of ethyl acetate-n-butanol-water (1:4:5, v/v) in the first run and chloroform-methanol-isopropanol-water (5:6:1:4) in the second run.
Xiao, Han, Tianyou, Zhang, Yun, Wei, Xueli, Cao, Yoichiro, Ito   +4 more
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Improved method for continuous uv monitoring in high-speed counter-current chromatography

Journal of Chromatography A, 1989
Continuous UV monitoring of the effluent in high-speed counter-current chromatography often encounters difficulty mainly due to the thermolabile nature of the mobile phase which tends to develop turbidity in the flow cell under a slight shift of the ambient temperature.
H, Oka, Y, Ito
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Separation of Rare Earth Element Radioisotopes by Reverse-Phase High-Speed Counter-Current Chromatography

Journal of Chromatography A, 2023
Analytical scale purification of rare earth element (REE) radioisotopes is typically accomplished using cation-exchange resins (e.g. AG 50W-X8) and high-performance liquid chromatography (HPLC). Despite the variety of improvements made since the development of this separation process in the 1950s, nearest neighbor separations remain a challenge, as ...
Mateusz Dembowski, Hope E. Rasmussen, John E. Rowley, Janelle E. Droessler, George S. Goff, Iain May   +5 more
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