Results 251 to 260 of about 56,372 (290)

Isolation of bacitracins A and F by high-speed counter-current chromatography

Journal of Chromatography A, 1991
The major components of bacitracin were separated and purified using high-speed counter-current chromatography (HSCCC). A systematic search for optimum two-phase solvent systems resulted in two systems: chloroform-ethanol-methanol-water (5:3:3:4) and chloroform-ethanol-water (5:4:3).
K, Harada, Y, Ikai, Y, Yamazaki, H, Oka, M, Suzuki, H, Nakazawa, Y, Ito   +6 more
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Preparative separation of grape skin polyphenols by high-speed counter-current chromatography

Food Chemistry, 2016
To develop an efficient method for large preparation of various individual polyphenols from white grape skins (Fernão Pires; Vitis vinifera) by preparative high-speed counter-current chromatography (HSCCC) and preparative-HPLC, an optimized preparative HSCCC condition with two-phase solvent system composed of Hex-EtOAc-H2O (1:50:50, v/v) was used to ...
Lanxin, Luo, Yan, Cui, Shuting, Zhang, Lingxi, Li, Yuanyuan, Li, Peiyu, Zhou, Baoshan, Sun   +6 more
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Dual-mode high-speed counter-current chromatography: retention, resolution and examples

Journal of Chromatography A, 1997
Counter-current chromatography (CCC) is a very versatile technique offering high resolution power in recovering very pure compounds from complex matrices. Dual-mode CCC where the phase role is reversed during the separation is investigated here; it ensures elution of all the injected species from the column while, unlike backflush, the separation is ...
M Agnely, D Thiébaut
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High-speed preparative counter-current chromatography with a coil planet centrifuge

Journal of Chromatography A, 1982
Abstract A compact table-top model of a flow-through coil planet centrifuge produces and efficient chromatagraphic separation of solutes on a preparative scale within 2–5 h. The use of a multi-layer coiled column promotes retention of the stationary phase with a high flow-rate of the mobile phase and permits almost universal application of ...
Yoichiro Ito, Jesse Sandlin, William G. Bowers   +2 more
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A review of the hydrodynamics of high-speed counter current chromatography

2023
This review outlines the current understanding of the flow behavior in high-speed counter-current chromatography (HSCCC). HSCCC has seen huge progress in modeling and predicting flow behavior, yet it still is not fully understood from a hydrodynamic perspective.
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Purification of paeoniflorin from Paeonia lactiflora Pall. by high-speed counter-current chromatography

Journal of Chromatography A, 2004
High-speed counter-current chromatography was successfully used for the first time for the preparative separation and purification of paeoniflorin from the Chinese medicinal plant Paeonia lactiflora Pall. using a two-phase solvent system composed of n-butanol-ethyl acetate-water (1:4:5, v/v) in a single run.
Chen, F, Lu, HT, Jiang, Y
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Isolation of hydroxycinnamoyltartaric acids from grape pomace by high-speed counter-current chromatography

Journal of Chromatography A, 2006
A method for the isolation of caftaric, coutaric and fertaric acids from grape pomace by high-speed counter-current chromatography (HSCCC) was developed. Using a system of hexane/ethyl acetate/methanol/water 3:7:3:7 (v/v/v/v) and 0.5% trifluoroacetic acid (TFA) in the head-to-tail elution mode, the target compounds were separated from co-extracted ...
Thorsten, Maier, Solveigh, Sanzenbacher, Dietmar R, Kammerer, Nicolai, Berardini, Jürgen, Conrad, Uwe, Beifuss, Reinhold, Carle, Andreas, Schieber   +7 more
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Isolation of dammarane saponins from Panax notoginseng by high-speed counter-current chromatography

Journal of Chromatography A, 2003
The Chinese phytomedicinal formulation Sanqi Zongdai Pian, traditionally prepared from crude extracts from roots of Panax notoginseng (Araliaceae), contains highly polar dammarane saponins which were separated at a preparative scale using high-speed counter-current chromatography (HSCCC). In each operation, 283 mg methanolic extract of five tablets was
Qizhen, Du, Gerold, Jerz, Reiner, Waibel, Peter, Winterhalter   +3 more
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Separation of proanthocyanidins isolated from tea leaves using high-speed counter-current chromatography

Journal of Chromatography A, 2009
The proanthocyanidin extract from tea (Camellia sinensis) leaves was purified for the further study of the biological role of proanthocyanidins in blister blight leaf disease of tea, which is caused by the fungus Exobasidium vexans. An aqueous acetone extract of proanthocyanidins prepared from healthy tea leaves was partially purified using Sephadex LH-
N, Savitri Kumar, W M A, Maduwantha B Wijekoon, Vijaya, Kumar, P A, Nimal Punyasiri, I, Sarath B Abeysinghe   +4 more
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Separation and purification of 10-deacetylbaccatin III by high-speed counter-current chromatography

Journal of Chromatography A, 1998
High-speed counter-current chromatography (HSCCC) was used for the separation and purification of 10-deacetylbaccatin III from the needle extract of Chinese yew (Taxus chinensis). The crude needle extract (500 mg/5 ml) was first separated with a two-phase solvent system composed of n-hexane-ethyl acetate-ethanol-water (2:5:2:5, v/v) and the partially ...
X, Cao, Y, Tian, T Y, Zhang, Y, Ito
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