Results 91 to 100 of about 273,042 (329)

A universal method for automated gene mapping [PDF]

, 2005
Small insertions or deletions (InDels) constitute a ubiquituous class of sequence polymorphisms found in eukaryotic genomes. Here, we present an automated high-throughput genotyping method that relies on the detection of fragment-length polymorphisms ...
Basler, Konrad, Hafen, Ernst, Hajnal, Alex, Hengartner, Michael, Nairz, Knud, Rimann, Ivo, Zipperlen, Peder   +6 more
core   +2 more sources

Additional file 3 of Correcting nucleotide-specific biases in high-throughput sequencing data

, 2017
Figure S2. ChIP-seq and DNase-seq coverage in a super enhancer region (Hnisz D, Abraham BJ, Lee TI, et al. Transcriptional super-enhancers connected to cell identity and disease. Cell. 2013;155(4):10.1016/j.cell.2013.09.053). This region is also in a DNase hypersensitivity region.
Wang, Jeremy, Quach, Bryan, Furey, Terrence   +2 more
openaire   +1 more source

High-throughput targeted long-read single cell sequencing reveals the clonal and transcriptional landscape of lymphocytes

Nature Communications, 2018
High-throughput single-cell RNA sequencing is a powerful technique but only generates short reads from one end of a cDNA template, limiting the reconstruction of highly diverse sequences such as antigen receptors. To overcome this limitation, we combined
Mandeep Singh, G. Al-Eryani, Shaun Carswell, James M. Ferguson, J. Blackburn, K. Barton, D. Roden, F. Luciani, Tri Giang Phan, S. Junankar, Katherine J. L. Jackson, C. Goodnow, Martin A. Smith, A. Swarbrick   +13 more
semanticscholar   +1 more source

Systemic T Cell Receptor Profiling Reveals Adaptive Immune Activation and Potential Immune Signatures of Diagnosis and Brain Atrophy in Epilepsy

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Epilepsy is increasingly associated with immune dysregulation and inflammation. The T cell receptor (TCR), a key mediator of adaptive immunity, shows repertoire alterations in various immune‐mediated diseases. The unique TCR sequence serves as a molecular barcode for T cells, and clonal expansion accompanied by reduced overall TCR ...
Yong‐Won Shin, Sang Bin Hong, Yong Woo Shin, Inpyeong Hwang, Jaeseong Oh, Jihyeon Choi, Narae Kim, Jangsup Moon, Keun‐Hwa Jung, Kyung‐Il Park, Ki‐Young Jung, Kon Chu, Sang Kun Lee   +12 more
wiley   +1 more source

The use of coded PCR primers enables high-throughput sequencing of multiple homolog amplification products by 454 parallel sequencing. [PDF]

PLoS ONE, 2007
The invention of the Genome Sequence 20 DNA Sequencing System (454 parallel sequencing platform) has enabled the rapid and high-volume production of sequence data.
Jonas Binladen, M Thomas P Gilbert, Jonathan P Bollback, Frank Panitz, Christian Bendixen, Rasmus Nielsen, Eske Willerslev   +6 more
doaj   +1 more source

Generation of large mitochondrial and nuclear nucleotide sequences and phylogenetic analyses using high-throughput short-read datasets for endangered Placostylinae snails of the southwest Pacific

Molluscan Research, 2021
Placostylinae are a sub-family of terrestrial land snails endemic to the southwest Pacific. Some species are harvested for food, and others are critically endangered. Here we assemble and characterise complete mitochondrial genomes, as well as three nuclear markers (partial 45S ribosomal cassettes and the histone genes H3 and H4) of five snail species ...
Mathieu Quenu, Steven A. Trewick, Elizabeth E. Daly, Mary Morgan-Richards   +3 more
openaire   +1 more source

Multiplex restriction amplicon sequencing: a novel next‐generation sequencing‐based marker platform for high‐throughput genotyping

Plant Biotechnology Journal, 2019
Summary To enable rapid selection of traits in marker‐assisted breeding, markers must be technically simple, low‐cost, high‐throughput and randomly distributed in a genome.
A. Bernardo, Paul St. Amand, Ha Le, Zhenqi Su, G. Bai   +4 more
semanticscholar   +1 more source

Laser‐Based Sculpturing of Embedded Ultrathin Metal‐Oxide Nanopores for Enhanced Biomolecular Sensing

Advanced Functional Materials, EarlyView.
Controlled laser‐drilling of embedded HfO2 membranes creates three layer nanopores with Gaussian‐shaped cavities sculptured in the supporting layers. These embedded solid‐state nanopores slow DNA translocation by 12‐fold compared to SiNx pores, enabling high‐resolution, label‐free detection of short DNAs, RNAs, and proteins.
Jostine Joby, Yulia Marom, Guocong Lin, Aleksandra Baron‐Wiechec, Amit Meller   +4 more
wiley   +1 more source

LinkedSV for detection of mosaic structural variants from linked-read exome and genome sequencing data

Nature Communications, 2019
Compared to single nucleotide variants and short indels, structural variants (SVs) are often more challenging to detect using high-throughput sequencing based methods. Here, the authors develop LinkedSV, a computational tool for SV detection using linked-
Li Fang, Charlly Kao, Michael V. Gonzalez, Fernanda A. Mafra, Renata Pellegrino da Silva, Mingyao Li, Sören-Sebastian Wenzel, Katharina Wimmer, Hakon Hakonarson, Kai Wang   +9 more
doaj   +1 more source

Genotyping by PCR and High-Throughput Sequencing of Commercial Probiotic Products Reveals Composition Biases.

Frontiers in Microbiology, 2016
Recent advances in microbiome research have brought renewed focus on beneficial bacteria, many of which are available in food and dietary supplements. Although probiotics have historically been defined as microorganisms that convey health benefits when ...
Wesley Morovic, Ashley A. Hibberd, Bryan Zabel, Rodolphe Barrangou, Buffy Stahl   +4 more
doaj   +1 more source