Results 101 to 110 of about 213,884 (275)

A high-throughput sequencing test for diagnosing inherited bleeding, thrombotic, and platelet disorders.

Blood, 2016
Inherited bleeding, thrombotic, and platelet disorders (BPDs) are diseases that affect ∼300 individuals per million births. With the exception of hemophilia and von Willebrand disease patients, a molecular analysis for patients with a BPD is often ...
Ilenia Simeoni, J. Stephens, Fengyuan Hu, Sri V. V. Deevi, K. Megy, T. Bariana, C. Lentaigne, Sol Schulman, S. Sivapalaratnam, Minka J. A. Vries, S. Westbury, D. Greene, S. Papadia, M. Alessi, A. Attwood, M. Ballmaier, G. Baynam, E. Bermejo, M. Bertoli, P. Bray, L. Bury, M. Cattaneo, P. Collins, L. Daugherty, R. Favier, D. French, B. Furie, M. Gattens, M. Germeshausen, C. Ghevaert, A. Goodeve, J. Guerrero, D. Hampshire, D. Hart, J. Heemskerk, Y. Henskens, M. Hill, Nancy Hogg, J. Jolley, W. Kahr, A. Kelly, R. Kerr, Myrto A. Kostadima, S. Kunishima, Michele P. Lambert, R. Liesner, José A López, R. Mapeta, M. Mathias, C. Millar, A. Nathwani, M. Neerman-Arbez, A. Nurden, P. Nurden, M. Othman, K. Peerlinck, D. Perry, Pawan Poudel, P. Reitsma, M. Rondina, P. Smethurst, W. Stevenson, A. Szkotak, Salih Tuna, C. van Geet, Deborah Whitehorn, D. Wilcox, Bin Zhang, S. Revel-Vilk, P. Gresele, D. Bellissimo, C. Penkett, M. Laffan, A. Mumford, A. Rendon, K. Gomez, K. Freson, W. Ouwehand, E. Turro   +78 more
semanticscholar   +1 more source

Microbiome Single Cell Atlases Generated with a Commercial Instrument

Advanced Science, EarlyView.
Single cell sequencing is valuable for dissecting complex systems and uncovering unique features in individual cell types. However, tools for single cell microbial analysis are limited. Easily Accessible Single microbe sequencing (EASi‐seq), which adapts a commercially available single cell platform for efficient sequencing of single microbial genomes ...
Xiangpeng Li, Linfeng Xu, Benjamin Demaree, Cecilia Noecker, Jordan E. Bisanz, Daniel W. Weisgerber, Cyrus Modavi, Peter J. Turnbaugh, Adam R. Abate   +8 more
wiley   +1 more source

β‐Glucuronidase‐Expressing Lactobacillus reuteri Triggers Irinotecan Enterotoxicity Through Depleting the Regenerative Epithelial Stem/Progenitor Pool

Advanced Science, EarlyView.
XLP mitigated CPT11 mucositis by suppressing GUS‐expressing microbes, notably L. reuteri, and diminishing bacterial GUS activity, consequently reducing SN38 accumulation to protect the intestinal epithelium. This preservation of the mucosal stem cell niche enabled rapid regeneration of secretory lineages such as mucin‐producing goblet cells, which ...
Bei Yue, Ruiyang Gao, Ling Zhao, Donghui Liu, Cheng Lv, Ziyi Wang, Fangbin Ai, Beibei Zhang, Zhilun Yu, Xiaolong Geng, Hao Wang, Kang Wang, Kaixian Chen, Chenghai Liu, Zhengtao Wang, Wei Dou   +15 more
wiley   +1 more source

Alnustone Ameliorates Metabolic Dysfunction‐Associated Steatotic Liver Disease by Facilitating Mitochondrial Fatty Acid β‐Oxidation via Targeting Calmodulin

Advanced Science, EarlyView.
This study identifies alnustone, a natural compound from Alpinia katsumadai, as a potent therapeutic agent for MASLD and MASH. Alnustone enhances mitochondrial fatty acid β‐oxidation by directly targeting calmodulin, improving liver steatosis, fibrosis, and insulin resistance in vivo.
Shourui Hu, Xiaofan Liang, Yiming Qin, Yuxuan Li, Yue Liu, Congcong Liu, Zongxuan Lin, Chunxuan Geng, Yanqi Xu, Daimin Wei, Yingying Qin, Han Zhao, Yuqing Zhang, Zi‐Jiang Chen   +13 more
wiley   +1 more source

Unbiased Characterization of Anopheles Mosquito Blood Meals by Targeted High-Throughput Sequencing

PLoS Neglected Tropical Diseases, 2016
Understanding mosquito host choice is important for assessing vector competence or identifying disease reservoirs. Unfortunately, the availability of an unbiased method for comprehensively evaluating the composition of insect blood meals is very limited,
K. Logue, John B. Keven, M. Cannon, L. Reimer, P. Siba, E. Walker, P. Zimmerman, D. Serre   +7 more
semanticscholar   +1 more source

IncRNA‐ZFAS1, an Emerging Gate‐Keeper in DNA Damage‐Dependent Transcriptional Regulation

Advanced Science, EarlyView.
LncZFAS1 plays a crucial role during DNA damage response in mammalian cells. Loss of lncZFAS1 results in deficient DNA lesion removal and reduced cell viability. Mechanistically, lncZFAS1 modulates RNAPII phosphorylation and transcription and thereby promotes both GG‐NER and TC‐NER upon UV damage.
Jiena Liu, Qing Lu, Zixuan Fan, Jiahui Lin, Nan He, Xin Zhang, Zhaoya Han, Tingting Zhu, Zhenzhen Wu, Yingying Xu, Yuming Wang   +10 more
wiley   +1 more source

Identification of miRNAs and their targets in wild tomato at moderately and acutely elevated temperatures by high-throughput sequencing and degradome analysis

Scientific Reports, 2016
MicroRNAs (miRNAs) are 19–24 nucleotide (nt) noncoding RNAs that play important roles in abiotic stress responses in plants. High temperatures have been the subject of considerable attention due to their negative effects on plant growth and development ...
R. Zhou, Qian Wang, F. Jiang, Xue Cao, Mintao Sun, Min Liu, Zhen Wu   +6 more
semanticscholar   +1 more source

Integrating Dense Genotyping with High‐Throughput Phenotyping Empowers the Genetic Dissection of Berry Quality and Resilience Traits in Grapevine

Advanced Science, EarlyView.
Researchers develop advanced tools to study grapevine traits like berry quality and stress resilience. A 200K SNP array and high‐throughput phenotyping enable the identification of loci linked to berry shape, sugar content, acidity, and cold tolerance. Functional validation of genes such as NAC08 reveals roles in cold tolerance.
Yuyu Zhang, Yongjian Wang, Michael Henke, Pablo Carbonell‐Bejerano, Zemin Wang, Pierre‐François Bert, Yi Wang, Huayang Li, Junhua Kong, Peige Fan, Zhanwu Dai, Zhenchang Liang   +11 more
wiley   +1 more source

Genetic landscape of myelodysplastic syndrome and its prognostic relevance: a study from Pakistan

Journal of the Pakistan Medical Association
Objective: To determine the genetic landscape of myelodysplastic syndrome patients, and to evaluate the impact of gene mutations on disease prognosis and overall survival.
Alia Waheed, Saleem Ahmed Khan, Rafia Mahmood, Hamid Saeed Malik, Humayoon Shafique Satti, Fehmida Farid Khan   +5 more
doaj   +1 more source

The efficacy of high-throughput sequencing and target enrichment on charred archaeobotanical remains

Scientific Reports, 2016
The majority of archaeological plant material is preserved in a charred state. Obtaining reliable ancient DNA data from these remains has presented challenges due to high rates of nucleotide damage, short DNA fragment lengths, low endogenous DNA content ...
H. Nistelberger, Oliver Smith, N. Wales, Bastiaan Star, S. Boessenkool   +4 more
semanticscholar   +1 more source