Results 121 to 130 of about 273,042 (329)

Protocol: a versatile, inexpensive, high-throughput plant genomic DNA extraction method suitable for genotyping-by-sequencing

Plant Methods, 2018
The recent development of next-generation sequencing DNA marker technologies, such as genotyping-by-sequencing (GBS), generates thousands of informative single nucleotide polymorphism markers in almost any species, regardless of genomic resources.
C. Anderson, Benjamin K. Franzmayr, Soon Won Hong, Anna Larking, T. V. van Stijn, Rachel Tan, Roger A. Moraga, M. Faville, A. Griffiths   +8 more
semanticscholar   +1 more source

Circular RNA PTPN4 Contributes to Blood‐Brain Barrier Disruption during Early Epileptogenesis

Advanced Science, EarlyView.
Epileptic condition induces CircPTPN4 upregulation, which promotes ECE‐1 expression through competitive sequestration of miR‐145a‐5p. The elevated ECE‐1 catalyzes the ET‐1 production, leading to p38/MAPK pathway activation and subsequent downregulation of tight junction protein expression. This cascade results in increased BBB permeability and enhanced
Jiurong Yang, Yang Hu, Feiyu Wang, Xintao Peng, Honggang Qi, Yuanyuan Yao, Canyu Zhang, Lijie Zhou, Xuemei Liang, Kang Xu, Cong Zhang, Aifeng Zhang, Chen Chen, Yu Zeng, Chenchen Zhang, Guangming Gan, Xinjian Zhu   +16 more
wiley   +1 more source

SInC: An accurate and fast error-model based simulator for SNPs, Indels and CNVs coupled with a read generator for short-read sequence data [PDF]

, 2013
We report SInC (SNV, Indel and CNV) simulator and read generator, an open-source tool capable of simulating biological variants taking into account a platform-specific error model. SInC is capable of simulating and generating single- and paired-end reads
Gupta, Saurabh, Panda, Binay, Pattnaik, Swetansu, Rao, Arjun A   +3 more
core   +2 more sources

BARCOSEL: a tool for selecting an optimal barcode set for high-throughput sequencing

BMC Bioinformatics, 2018
Current high-throughput sequencing platforms provide capacity to sequence multiple samples in parallel. Different samples are labeled by attaching a short sample specific nucleotide sequence, barcode, to each DNA molecule prior pooling them into a mix ...
P. Somervuo, Patrik Koskinen, P. Mei, L. Holm, P. Auvinen, L. Paulin   +5 more
semanticscholar   +1 more source

PHR‐Mediated Pi Starvation Response Mobile Messenger RNAs Represent Noncoding Transcripts in Recipient Tissues

Advanced Science, EarlyView.
Mobile messenger RNAs (mRNAs) can travel long distances, serving as systemic signals that participate in plant growth and stress acclimation. This work determines that PHR proteins mediate the Pi starvation response (PSR)‐specific long‐distance transport of mRNAs and that these PSR‐specific mobile mRNAs represent noncoding transcripts in recipient ...
Weiguo Dong, Shuman Wang, Chao Liu, Lulu Qiao, Kai Liu, Qiongli Jin, Xiaorong Mo, Xingxing Shen, Keke Yi, Zhiye Wang   +9 more
wiley   +1 more source

Molecular profiling of head and neck squamous cell carcinomas in North-eastern Italy identifies possible tumour cell vulnerabilities

Translational Oncology
Background and Purpose: Head and Neck Squamous Cell Cancer (HNSCC) originates from the oral cavity, oropharynx, hypopharynx and larynx, and it ranks sixth among global cancers.
Monica Schiappacassi, Riccardo Spizzo, Jerry Polesel, Lorena Musco, Roberto Doliana, Luca Pellizzari, Valentina Lupato, Giuseppe Fanetti, Emanuela Vaccher, Diego Serraino, Luigi Barzan, Sandro Sulfaro, Vittorio Giacomarra, Giovanni Franchin, Gustavo Baldassarre   +14 more
doaj   +1 more source

EIF1AX Nucleolar Condensates Enhance Susceptibilities for the Management of Endometrial Cancer

Advanced Science, EarlyView.
This schematic illustrates the mechanism of a senolytic strategy in endometrial cancer. EIF1AX facilitates the incorporation of DDX21 into nucleolar condensates, an event that suppresses rDNA transcription and induces cellular senescence. The compound 2,5‐MeC exploits this pathway by promoting EIF1AX nucleolar translocation and condensate formation ...
Chengyu Lv, Zihang Lin, Jiandong Sun, Yuhong Ye, Qibin Wu, Liangzhi Cai, Dabin Liu, Pengming Sun, Shie Wang   +8 more
wiley   +1 more source

Additional file 6: of Uncommon nucleotide excision repair phenotypes revealed by targeted high-throughput sequencing

, 2016
Clinical pictures and brain MRI of patients #16 (mutated in ERCC5(XPG)). Sagittal T1 and axial T2-Flair (PPTX 970 kb)
Calmels, Nadège, Greff, Géraldine, Obringer, Cathy, Kempf, Nadine, Gasnier, Claire, Tarabeux, Julien, Miguet, Marguerite, Baujat, Geneviève, Bessis, Didier, Bretones, Patricia, Cavau, Anne, Digeon, Béatrice, Doco-Fenzy, Martine, Doray, Bérénice, Feillet, François, Gardeazabal, Jesus, Gener, Blanca, Julia, Sophie, Llano-Rivas, Isabel, Mazur, Artur, Michot, Caroline, Renaldo-Robin, Florence, Rossi, Massimiliano, Sabouraud, Pascal, Keren, Boris, Depienne, Christel, Muller, Jean, Jean-Louis Mandel, Laugel, Vincent   +28 more
openaire   +1 more source

Using high-throughput barcode sequencing to efficiently map connectomes

bioRxiv, 2017
The function of a neural circuit is determined by the details of its synaptic connections. At present, the only available method for determining a neural wiring diagram with single synapse precision—a “connectome”—is based on imaging methods that are ...
Ian D. Peikon, Justus M. Kebschull, Vasily V. Vagin, Diana I. Ravens, Yu-Chi Sun, Eric Brouzes, Ivan R. Corrêa, D. Bressan, A. Zador   +8 more
semanticscholar   +1 more source

TSHR‐Targeting Nucleic Acid Aptamer Treats Graves' Ophthalmopathy via Novel Allosteric Inhibition

Advanced Science, EarlyView.
This study presents YC3, a novel inhibitory TSHR‐targeting aptamer, as a promising therapeutic for Graves' ophthalmopathy (GO). YC3 suppresses pathological phenotypes in human orbital fibroblasts and improves outcomes in GO mice by binding to a previously unidentified allosteric site on TSHR, demonstrating the potential of aptamers in advancing ...
Yanchen Zhang, Ende Wu, Weibin Liu, Ling Zeng, Neng Ling, Hongmei Wang, Zhixing Li, Shuang Yao, Tonghe Pan, Xuanwen Li, Yate Huang, Xiaojing Li, Yunhai Tu, Wentao Yan, Jianzhang Wu, Mao Ye, Wencan Wu   +16 more
wiley   +1 more source