Results 121 to 130 of about 213,884 (275)

CRISPR-sub: Analysis of DNA substitution mutations caused by CRISPR-Cas9 in human cells

Computational and Structural Biotechnology Journal, 2020
CRISPR-Cas9 induces DNA cleavages at desired target sites in a guide RNA-dependent manner; DNA editing occurs through the resulting activity of DNA repair processes including non-homologous end joining (NHEJ), which is dominant in mammalian cells.
Gue-Ho Hwang, Jihyeon Yu, Soyeon Yang, Woo Jae Son, Kayeong Lim, Heon Seok Kim, Jin-Soo Kim, Sangsu Bae   +7 more
doaj  

Analysis of the Repertoire Features of TCR Beta Chain CDR3 in Human by High-Throughput Sequencing

Cellular Physiology and Biochemistry, 2016
Background/Aims: To ward off a wide variety of pathogens, the human adaptive immune system harbors a vast array of T-cell receptors, collectively referred to as the TCR repertoire.
X. Hou, Mingbang Wang, Chong Lu, Q. Xie, G. Cui, Jianing Chen, Yu Du, Y. Dai, H. Diao   +8 more
semanticscholar   +1 more source

Additional file 3: of Uncommon nucleotide excision repair phenotypes revealed by targeted high-throughput sequencing

, 2016
Novel POLH splice mutation in non-related XP-variant patients. A: Pedigrees of the 4 XP-variant families coming from Northern Spain and mutated in the POLH gene. The arrows indicate the four patients studied by NGS. Patients #11, 12 and 13 were homozygous for the splice mutation c.764 + 1G > A whereas patient #10 was compound heterozygous for ...
Calmels, Nadège, Greff, Géraldine, Obringer, Cathy, Kempf, Nadine, Gasnier, Claire, Tarabeux, Julien, Miguet, Marguerite, Baujat, Geneviève, Bessis, Didier, Bretones, Patricia, Cavau, Anne, Digeon, Béatrice, Doco-Fenzy, Martine, Doray, Bérénice, Feillet, François, Gardeazabal, Jesus, Gener, Blanca, Julia, Sophie, Llano-Rivas, Isabel, Mazur, Artur, Michot, Caroline, Renaldo-Robin, Florence, Rossi, Massimiliano, Sabouraud, Pascal, Keren, Boris, Depienne, Christel, Muller, Jean, Jean-Louis Mandel, Laugel, Vincent   +28 more
openaire   +1 more source

From Code to Life: The AI‐Driven Revolution in Genome Editing

Advanced Science, EarlyView.
This review explores AI‐driven advancements in genome editing, focusing on CRISPR optimization, enhanced targeting precision, reduced off‐target effects, and novel tool design. It addresses ethical challenges, data biases, and highlights future prospects in precision medicine, agriculture, and synthetic biology.
Zhidong Li, Wasi Ullah Khan, Genxiang Bai, Chao Dong, Jungang Wang, Youpeng Zhang, Chong Wang, Hongbin Zhang, Wenyi Wang, Ming Luo, Fei Chen   +10 more
wiley   +1 more source

Genome-wide identification of microRNAs in pomegranate (Punica granatum L.) by high-throughput sequencing

BMC Plant Biology, 2016
MicroRNAs (miRNAs), a class of small non-coding endogenous RNAs that regulate gene expression post-transcriptionally, play multiple key roles in plant growth and development and in biotic and abiotic stress response.
T. Saminathan, Abiodun Bodunrin, N. Singh, Ramajayam Devarajan, P. Nimmakayala, Moersfelder Jeff, M. Aradhya, U. Reddy   +7 more
semanticscholar   +1 more source

Small RNA Toxin‐Assisted Evolution of GC‐Preferred ErCas12a for Enhanced Genome Targeting Range

Advanced Science, EarlyView.
ErCas12a is engineered to target GC‐rich PAMs using a small RNA toxin‐aided positive screening system. The resulting variant, enErCas12a, exhibits an expanded PAM profile and facilitates efficient gene editing in both bacterial and mammalian cells, while preserving high targeting specificity for both canonical and non‐canonical PAM targets.
Zehua Chen, Junyuan Xue, Ziying Wang, Jinyuan Sun, Yinglu Cui, Tong Zhu, Huaiyi Yang, Ming Li, Bian Wu   +8 more
wiley   +1 more source

NIR Driven Pd/Cerium Oxide Nano‐Heterojunction for Enhanced Salvaging Sepsis Induced Acute Liver Injury via Reprogramming Redox Homeostasis in Synergy with Inducing Autophagy

Advanced Science, EarlyView.
In vivo SALI therapy is achieved by scavenging intracellular ROS levels, downregulating inflammatory factors expression levels, inducing macrophage M2 directional polarization, and activating Keap1/Nrf‐2/HO‐1 pathway to reprogram redox homeostasis, induce cellular autophagy, reduce systemic inflammation, and promote liver tissue repair. Abstract Sepsis
Tao Qin, Lian Qin, Yang Zhao, Yin Chen, Qianyue Liu, Xiaoguang Lin, Yongfeng Lan, Yaohui Huang, Yan Liu, Ke Zhang, Lifan Pan, Jiaxiao Li, Kunpeng Duan, Hao Liang, Mingjing Yin, Guiyang Fan, Lian Liu, Yu Deng, Lin Liao, Danke Su, Ming Gao, Junyu Lu   +21 more
wiley   +1 more source

Recent Advances in mRNA Delivery Systems for Cancer Therapy

Advanced Science, EarlyView.
This review systematically investigates the applications of mRNA therapy in cancer treatment, with particular emphasis on nonviral delivery systems, targeting strategies, stimulus‐responsive systems, and local delivery methods. Concluding with a meticulous evaluation, the review sheds light on the prevailing challenges while illuminating promising ...
Zheng Zhang, Ya‐Nan Fan, Si‐Qi Jiang, Ya‐Jing Ma, Yao‐Ru Yu, Yu‐Xin Qing, Qian‐Ru Li, Yi‐Lin Liu, Song Shen, Jun Wang   +9 more
wiley   +1 more source

Nanoparticle‐Mediated CXCL12–CXCR4 Inhibition Reprograms Macrophages and Suppresses Gastric Carcinoma

Advanced Science, EarlyView.
Molecular mechanism study of self‐assembled M2pep‐Cs NPs/Plerixafor nanoparticles in targeting MΦ reprogramming by regulating the CXCL12–CXCR4 signaling pathway to enhance immune recognition and clearance of GC (Created by BioRender). Abstract Gastric carcinoma (GC) remains a major global health challenge, requiring novel therapeutic approaches.
Qianqian Cao, Xiaolei Cheng, Rongbin Lv, Dianshui Sun, Jihua Wang, Runjia Fu, Rumei Gong, Yueying Xiao, Qin Liu, Xiaomei Li   +9 more
wiley   +1 more source

MINSEQE: Minimum Information about a high-throughput Nucleotide SeQuencing Experiment - a proposal for standards in functional genomic data reporting

, 2012
MINSEQE describes the Minimum Information about a high-throughput nucleotide SEQuencing Experiment that is needed to enable the unambiguous interpretation and facilitate reproduction of the results of the experiment. By analogy to the MIAME guidelines for microarray experiments, adherence to the MINSEQE guidelines will improve integration of multiple ...
Brazma, Alvis, Ball, Catherine, Bumgarner, Roger, Furlanello, Cesare, Miller, Michael, Quackenbush, John, Reich, Michael, Rustici, Gabriella, Stoeckert, Chris, Trutane, Stephen Chervitz, Taylor, Ronald C   +10 more
openaire   +1 more source