Results 131 to 140 of about 149,249 (267)

Retromer Regulates Macro‐ and Micro‐Autophagy via Distinct Vacuolar Proteases in the Rice Blast Fungus

Advanced Science, EarlyView.
In wild‐type of M. oryzae (Guy11), MoPrb1 and MoPep4 are delivered into the vacuole by the retromer complex to mediate protein degradation and selective autophagy. Their proper localization and catalytic functions are essential for the fungal vegetative growth, conidiation and pathogenicity. However, retromer mutations disrupt this transport, impairing
Dingyang Zhang, Jiexiong Hu, Yonghe Hong, Yuping Fan, Minghui Peng, Yakubu Saddeeq Abubakar, Shujing Liang, Nan Jiang, Lili Lin, Xiaohong Pan, Huakun Zheng, Naweed I. Naqvi, Zonghua Wang, Wenhui Zheng   +13 more
wiley   +1 more source

CRISPR‐DNA Polymerase Assisted Targeted Mutagenesis for Regulable Laboratory Evolution

Advanced Science, EarlyView.
This study develops an in vivo targeted mutagenesis system based on CRISPR‐guided bacteriophage T5 or T7 DNA polymerases. This system can enable all possible types of substitution mutations within an expanded mutagenesis window in Escherichia coli and exhibit application potential in different microorganisms, thus accelerating the evolution of ...
Shuaili Chen, Xiangdi Chen, Yifan Peng, Qinghua Li, Jingwen Zhou, Jianghua Li, Guocheng Du, Jian Chen, Guoqiang Zhang   +8 more
wiley   +1 more source

Unveiling Novel Viral Diversity, Biogeography, and Host Networks in Wildlife Through High‐Throughput Sequencing Data Mining

Advanced Science, EarlyView.
Analysis of 57 536 high‐throughput sequencing datasets uncovers a vast, hidden world of viruses in wildlife. The researchers reveal significant geographic and host‐specific patterns of viruses, and their surprising cross‐species transmissions, such as avian flu viruses infecting goats.
Hai Wang, Yafei Meng, Xiaoyuan Chen, Xinyuan Cui, Qian Zuo, Na Han, Xianghui Liang, Xuejuan Shen, Caiwu Li, Desheng Li, Fumin Wang, Liangping He, Rujian Chen, Xingbang Lu, Wenjie You, Aiping Wu, Rui‐Ai Chen, Wu Chen, Chan Ding, Yongyi Shen   +19 more
wiley   +1 more source

EasyAmplicon 2: Expanding PacBio and Nanopore Long Amplicon Sequencing Analysis Pipeline for Microbiome

Advanced Science, EarlyView.
EasyAmplicon 2 is an updated, user‐friendly, and community‐supported pipeline designed for full‐length amplicon data from long‐read sequencing technologies such as PacBio and Nanopore. It supports multiple platforms (Illumina, PacBio, Nanopore, etc.), integrates tools like DADA2 and Emu, and offers a complete workflow from raw data processing to high ...
Hao Luo, Defeng Bai, Zhihao Zhu, Salsabeel Yousuf, Haifei Yang, Jiani Xun, Meiyin Zeng, Yao Wang, Yunyun Gao, Kai Peng, Shanshan Xu, Yuanping Zhou, Tianyuan Zhang, Chuang Ma, Huiyu Hou, Xiulin Wan, Yang Zhou, Baolei Jia, Shi Huang, Renyou Gan, Tao Wen, Tong Chen, Xia Chen, Xiaofang Li, Yong‐Xin Liu   +24 more
wiley   +1 more source

T‐2 Toxin Exploits Gut‐Derived Staphylococcus Saprophyticus to Disrupt Hepatic Macrophage Homeostasis

Advanced Science, EarlyView.
T‐2 toxin promotes the proliferation of intestinal S. saprophyticus and facilitates its translocation to the liver in piglets and mice. The translocated bacteria trigger autophagy in Kupffer cells and recruit monocytes to the liver through the nucleotide‐binding oligomerization domain 2 (NOD2) signaling pathway, alongside the M1 polarization of hepatic
Yuanyuan Zhu, Liu Xu, Fangrui Guo, Jianyu Qu, Xiangyan Liu, Qiurong Xu, Jie Sheng, Jiangping Wang, Xiaohong Xie, Ruimin Ren, Chuan Zhou, Sisi Yan, Shuiping Liu, Zhihang Yuan, Rongfang Li, Jing Wu, Jine Yi, Yulong Yin, Lixin Wen, Ji Wang   +19 more
wiley   +1 more source

Correction to: Evaluation of a High-Throughput Dense Single-Nucleotide Polymorphism PCR Multiplex Next Generation Sequencing Application for Human Remains Identification, by Radecke, et al. Forensic Genomics 2023;3(3):75–93; doi: 10.1089/forensic.2023.0005

, 2023

openalex   +1 more source

Phenylboronic Acid‐Modified Copper Nanozymes as Nanoscavengers of Bacterial Polysaccharides Causing Acute Lung Injury

Advanced Science, EarlyView.
A novel copper nanocluster coated with 4‐mercaptobenzeneboronic acid (CuMPBA) is developed to target bacterial polysaccharides for addressing complicated acute lung injury (ALI) caused by severe burns and early pulmonary infections. CuMPBA exhibits dual anti‐inflammatory and antibacterial mechanisms by: (a) binding and degrading toxic bacterial ...
Haojie Ge, Min Wang, Haijiao Xie, Xu‐Lin Chen, Xianwen Wang   +4 more
wiley   +1 more source

Comprehensive Measurement of Inter‐Individual Variation in DNA Repair Capacity in Healthy Individuals

Advanced Science, EarlyView.
Susceptibility to the health effects of DNA damage depends on a person's ability to repair DNA damage, but DNA repair has been difficult to measure in populations. A functional assay called FM‐HCR is used to measure DNA repair in 6 major pathways across 56 individuals.
Ting Zhai, Patrizia Mazzucato, Catherine Ricciardi, David C. Christiani, Liming Liang, Leona D. Samson, Isaac A. Chaim, Zachary D. Nagel   +7 more
wiley   +1 more source

A Forward Genetics Strategy for High‐Throughput Gene Identification via Precise Image‐Based Phenotyping of an Indexed EMS Mutant Library

Advanced Science, EarlyView.
The GeneHunter‐Gene‐Level Association (GH‐GLA) pipeline enables high‐throughput gene identification in an indexed EMS population of wheat cultivar KN9204. It identifies 5905 trait‐associated wheat genes and validates key regulators of kernel weight and spikelet architecture via gene editing and haplotype analysis.
Haojie Wang, Fujun Sun, Zeyu Shi, Yufeng Yang, Yi Ding, Tengteng Zhang, Caihong Zhao, Jingzhong Xie, Yaqian Zhang, Caihua Li, Wenqiang Tang, Junming Li, Xigang Liu, Shusong Zheng, Ni Jiang, Fei He, Shuzhi Zheng   +16 more
wiley   +1 more source

Mapping the Non‐Canonical Splicing Variants: Decrypting the Hidden Genetic Architecture of Idiopathic Male Infertility

Advanced Science, EarlyView.
This study highlights the significance of non‐canonical splicing variants in male infertility, a factor often overlooked during the analysis of high‐throughput sequencing data. Incorporating the non‐canonical splicing variants prioritization in the genetic analysis pipeline will increase the genetic diagnosis of patients with male infertility ...
Kuokuo Li, Yuge Chen, Dongdong Tang, Yuying Sheng, Xu Han, Hao Geng, Na Zhang, Zongliu Duan, Guanxiong Wang, Yang Gao, Rui Guo, Rong Hua, Zhiming Ding, Chuan Xu, Qunshan Shen, Zhen Yu, Bing Song, Mingrong Lv, Yuping Xu, Huan Wu, Ji Wu, Yunxia Cao, Xiaojin He   +22 more
wiley   +1 more source