Results 141 to 150 of about 273,042 (329)

Synthetic spike-in standards for high-throughput 16S rRNA gene amplicon sequencing

Nucleic Acids Research, 2016
High-throughput sequencing of 16S rRNA gene amplicons (16S-seq) has become a widely deployed method for profiling complex microbial communities but technical pitfalls related to data reliability and quantification remain to be fully addressed.
Dieter M. Tourlousse, Satowa Yoshiike, Akiko Ohashi, S. Matsukura, N. Noda, Y. Sekiguchi   +5 more
semanticscholar   +1 more source

5‐Hydroxymethylcytosine Dynamics Reveals Coordinated Reprogramming of Parental Genomes and X Chromosome Dosage Balance in Mouse SCNT Embryos

Advanced Science, EarlyView.
Genome‐wide, high‐resolution profiling of hydroxymethylation in mouse SCNT embryos reveals a transient, allele‐symmetric 5hmC reprogramming pattern distinct from natural embryos, with X‐chromosomes and imprinting control regions resistant to proper remodeling.
Zeming Xiang, Rui Yan, Jing Guo, Mengyao Wang, Xin Cheng, Fan Zhang, Tianzi Guo, Xin Long, Fan Guo, Dan Liang   +9 more
wiley   +1 more source

Genetic landscape of myelodysplastic syndrome and its prognostic relevance: a study from Pakistan

Journal of the Pakistan Medical Association
Objective: To determine the genetic landscape of myelodysplastic syndrome patients, and to evaluate the impact of gene mutations on disease prognosis and overall survival.
Alia Waheed, Saleem Ahmed Khan, Rafia Mahmood, Hamid Saeed Malik, Humayoon Shafique Satti, Fehmida Farid Khan   +5 more
doaj   +1 more source

Additional file 3: of Uncommon nucleotide excision repair phenotypes revealed by targeted high-throughput sequencing

, 2016
Novel POLH splice mutation in non-related XP-variant patients. A: Pedigrees of the 4 XP-variant families coming from Northern Spain and mutated in the POLH gene. The arrows indicate the four patients studied by NGS. Patients #11, 12 and 13 were homozygous for the splice mutation c.764 + 1G > A whereas patient #10 was compound heterozygous for ...
Calmels, Nadège, Greff, Géraldine, Obringer, Cathy, Kempf, Nadine, Gasnier, Claire, Tarabeux, Julien, Miguet, Marguerite, Baujat, Geneviève, Bessis, Didier, Bretones, Patricia, Cavau, Anne, Digeon, Béatrice, Doco-Fenzy, Martine, Doray, Bérénice, Feillet, François, Gardeazabal, Jesus, Gener, Blanca, Julia, Sophie, Llano-Rivas, Isabel, Mazur, Artur, Michot, Caroline, Renaldo-Robin, Florence, Rossi, Massimiliano, Sabouraud, Pascal, Keren, Boris, Depienne, Christel, Muller, Jean, Jean-Louis Mandel, Laugel, Vincent   +28 more
openaire   +1 more source

Heap: a highly sensitive and accurate SNP detection tool for low-coverage high-throughput sequencing data

DNA Research, 2017
Recent availability of large-scale genomic resources enables us to conduct so called genome-wide association studies (GWAS) and genomic prediction (GP) studies, particularly with next-generation sequencing (NGS) data.
Masaaki Kobayashi, Hajime Ohyanagi, H. Takanashi, Satomi Asano, Toru Kudo, H. Kajiya-Kanegae, A. Nagano, H. Tainaka, Tsuyoshi Tokunaga, T. Sazuka, H. Iwata, N. Tsutsumi, K. Yano   +12 more
semanticscholar   +1 more source

Lactylation‐Driven YTHDC1 Alleviates MASLD by Suppressing PTPN22‐Mediated Dephosphorylation of NLRP3

Advanced Science, EarlyView.
In MASLD, YTHDC1 undergoes increased lactylation and ubiquitination, reducing its expression. AARS1 mediates lactylation at lysine 565, while disrupted binding to LDHA further promotes lactylation, suppressing YTHDC1. This downregulation enhances PTPN22 mRNA stability, leading to NLRP3 dephosphorylation and activation, which exacerbates inflammation ...
Feng Zhang, Linghua Zeng, Kunkun Zou, Keying Lin, Fangting Yao, Jinglun Song, Shumeng Zhang, Hanshu Feng, Zhichao Yang, Chunlei Wang, Hongtao Diao, Xue Kong, Tengfei Pan, Joonki Kim, Tianqi Duo, Linqiang Li, Yu Bian   +16 more
wiley   +1 more source

Additional file 1: of Quantification of experimentally induced nucleotide conversions in high-throughput sequencing datasets

, 2019
Supplementary information, figures and tables. (PDF 6280 kb)
Neumann, Tobias, Herzog, Veronika, Muhar, Matthias, Haeseler, Arndt, Zuber, Johannes, Ameres, Stefan, Rescheneder, Philipp   +6 more
openaire   +1 more source

Targeting Itga8 Mitigates Neurogenic Bladder Fibrosis Driven by Trem2⁺ Macrophage‐Derived Fn1 via FAK/RhoA/ROCK Signaling

Advanced Science, EarlyView.
Normal bladders exhibit quiescent fibroblasts/macrophages, whereas neurogenic bladders show acute‐phase Itga8⁺ fibroblast expansion driven by Trem2⁺ macrophage‐secreted Fn1, which activates FAK/RhoA/ROCK signaling, promotes cytoskeletal remodeling, and upregulates pro‐fibrotic genes.
Jiaxin Wang, Siyuan Wang, Lida Ren, Xinqi Liu, Lei Zhang, Peng Hu, Wenchao Xu, Shuaixiang Zheng, Jihong Liu, Qing Ling   +9 more
wiley   +1 more source

Allelic Variation in Maize Malate Dehydrogenase 7 Shapes Promoter Methylation and Banded Leaf and Sheath Blight Resistance

Advanced Science, EarlyView.
In this study maize chloroplastic malate dehydrogenase7 (ZmMDH7), is identified as a Rhizoctonia solani resistance gene in maize. ZmMDH7 is regulated by transcription factor ZmWRKY44 via pathogens challenge to elevate mitochondrial ROS and SA signaling pathway.
Luyang Wei, Junbin Chen, Chuang Liu, DanDan Liu, Meida Du, Shengfeng He, Wenyu Cheng, Vijai Bhadauria, You‐Liang Peng, Wangsheng Zhu   +9 more
wiley   +1 more source

"Sequencing-grade" screening for BRCA1 variants by oligo-arrays

Journal of Translational Medicine, 2008
The need for fast, efficient, and less costly means to screen genetic variants associated with disease predisposition led us to develop an oligo-nucleotide array-based process for gene-specific single nucleotide polymorphism (SNP) genotyping.
Fasano Ross, Sabatino Marianna, Tommasi Stefania, Zhao Yingdong, Menolascina Filippo, Monaco Alessandro, Paradiso Angelo, Marincola Francesco M, Wang Ena   +8 more
doaj   +1 more source