Improved COI taxonomic assignment workflow enhances detection of invertebrates in environmental DNA
Limnology and Oceanography: Methods, EarlyView.Abstract Environmental DNA (eDNA) metabarcoding is an increasingly popular and powerful tool in ecology, offering a non‐invasive method to assess biodiversity and monitor ecosystem health across diverse environments. While the mitochondrial cytochrome c oxidase subunit I (COI) gene is one of the most widely used markers for studying eukaryotic ...
Yuanyu Cheng +5 more
wiley +1 more source
High-throughput virome profiling reveals complex viral diversity, co-infection patterns, and novel viruses in <i>Cnidium officinale</i> in Korea. [PDF]
Front Plant SciKang JH +5 more
europepmc +1 more source
Mass Spectrometry Structural Proteomics Enabled by Limited Proteolysis and Cross‐Linking
Mass Spectrometry Reviews, EarlyView.ABSTRACT The exploration of protein structure and function stands at the forefront of life science and represents an ever‐expanding focus in the development of proteomics. As mass spectrometry (MS) offers readout of protein conformational changes at both the protein and peptide levels, MS‐based structural proteomics is making significant strides in the
Haiyan Lu +4 more
wiley +1 more source
Beyond PD‐1/PD‐L1: New Immune Checkpoints and Therapeutic Combinations in Cancer Immunotherapy
Medicine Bulletin, EarlyView.ABSTRACT Recently, immune checkpoint inhibitors (ICIs), particularly PD‐1/PD‐L1 and CTLA‐4 inhibitors, have revolutionized cancer treatment, significantly improving survival rates for various malignancies. However, ICI therapies targeting single checkpoints on T cells still face numerous challenges, such as low response rates and post‐treatment ...
Yangyang Li, Zizhen Kang, Yanyun Du
wiley +1 more source
Poly(A) tail regulation in stem cells and early development. [PDF]
Cell RegenMiao X, Hu G.
europepmc +1 more source
CRISPR Enabled Precision Oncology: From Gene Editing to Tumor Microenvironment Remodeling
Med Research, EarlyView.CRISPR technology has progressed from a prokaryotic immune system to a diverse suite of editing platforms, including Cas nucleases, base and prime editors, and RNA‐targeting enzymes. These advances enable precise genomic and epigenomic interventions, high‐throughput functional screening, and immune engineering.
Kailai Li +8 more
wiley +1 more source
<i>TP53</i> Mutations in Mantle Cell Lymphoma: From Backup to Game Changer. [PDF]
J Clin MedCarazzolo ME, Moioli A, Visco C.
europepmc +1 more source
Integrating Long‐Read Nanopore Sequencing for Precision Resolution of Genomic Variants in Dystonia
Movement Disorders, EarlyView.Abstract Background Although many individuals with dystonia present with features indicative of single‐gene etiologies, obtaining definitive genetic diagnoses can be challenging. Objective We assessed the value of nanopore‐based long‐read sequencing (LRS) in achieving molecular clarification of dystonic syndromes.
Ugo Sorrentino +30 more
wiley +1 more source
PAM-readID is a rapid, simple, and accurate PAM determination method for CRISPR-Cas enzymes in mammalian cells. [PDF]
Commun BiolWang Q +10 more
europepmc +1 more source