Results 41 to 50 of about 149,249 (267)

Melon Transcriptome Characterization: Simple Sequence Repeats and Single Nucleotide Polymorphisms Discovery for High Throughput Genotyping across the Species [PDF]

The Plant Genome, 2011
Melon (Cucumis melo L.) ranks among the highest-valued fruit crops worldwide. Some genomic tools are available for this crop, including a Sanger transcriptome. We report the generation of 689,054 C. melo high-quality expressed sequence tags (ESTs) from two 454 sequencing runs, using normalized and nonnormalized complementary DNA (cDNA) libraries ...
Blanca Postigo, José Miguel, Cañizares Sales, Joaquín, Ziarsolo Areitioaurtena, Pello, Esteras Gómez, Cristina, Mir, Gisela, Nuez Viñals, Fernando, Garcia-Mas, Jordi, Picó Sirvent, María Belén   +7 more
openaire   +4 more sources

High‐throughput capture of nucleotide sequence polymorphisms in three Brassica species (Brassicaceae) using DNA microarrays [PDF]

American Journal of Botany, 2012
• Premise of the study: To capture molecular markers that are applicable to environmental risk assessment of genetically modified oilseed rape, and to streamline their development, we screened variations in nucleotide sequences of three Brassica species by DNA microarray analysis.• Methods and Results: Using the Affymetrix GeneChip Arabidopsis ATH1 ...
Nobuyoshi Nakajima, Akihiro Kubo, Masanori Tamaoki, Yukio Kaneko, Hikaru Saji, Toru Nishizawa, Mitsuko Aono   +6 more
openaire   +3 more sources

A bioinformatics approach to microRNA-sequencing analysis

Osteoarthritis and Cartilage Open, 2021
The rapid expansion of Next Generation Sequencing (NGS) data availability has made exploration of appropriate bioinformatics analysis pipelines a timely issue.
Pratibha Potla, Shabana Amanda Ali, Mohit Kapoor   +2 more
doaj   +1 more source

Genetic, Clinicopathological, and Radiological Features of Intrahepatic Cholangiocarcinoma with Ductal Plate Malformation Pattern

Gut and Liver, 2022
Background/Aims: Intrahepatic cholangiocarcinoma (iCCA) with a ductal plate malformation (DPM) pattern is a recently recognized rare variant. The genomic profile of iCCA with DPM pattern needs to be elucidated.
Taek Chung, Hyungjin Rhee, Hyo Sup Shim, Jeong Eun Yoo, Gi Hong Choi, Haeryoung Kim, Young Nyun Park   +6 more
doaj   +1 more source

CRISPR-sub: Analysis of DNA substitution mutations caused by CRISPR-Cas9 in human cells

Computational and Structural Biotechnology Journal, 2020
CRISPR-Cas9 induces DNA cleavages at desired target sites in a guide RNA-dependent manner; DNA editing occurs through the resulting activity of DNA repair processes including non-homologous end joining (NHEJ), which is dominant in mammalian cells.
Gue-Ho Hwang, Jihyeon Yu, Soyeon Yang, Woo Jae Son, Kayeong Lim, Heon Seok Kim, Jin-Soo Kim, Sangsu Bae   +7 more
doaj   +1 more source

Chromosomal analyses of human giant diploid oocytes by next‐generation sequencing

Reproductive Medicine and Biology, 2021
Purpose Although giant oocytes (GOs) having about twice cytoplasmic volume compared with general oocytes in mammals including the human are rarely recovered, it is thought that GOs have potentially chromosomal abnormalities.
Hiroomi Kawano, Naoki Yamashita, Junya Ito, Naomi Kashiwazaki   +3 more
doaj   +1 more source

Detection of Persistent Viruses by High-Throughput Sequencing in Tomato and Pepper from Panama: Phylogenetic and Evolutionary Studies

Plants, 2021
High-throughput sequencing from symptomatic tomato and pepper plants collected in Panama rendered the complete genome of the southern tomato virus (isolate STV_Panama) and bell pepper endornavirus (isolate BPEV_Panama), and almost-complete genomes of ...
Luis Galipienso, Laura Elvira-González, Leonardo Velasco, José Ángel Herrera-Vásquez, Luis Rubio   +4 more
doaj   +1 more source

Multidimensional analysis of the host response reveals prognostic and pathogen-driven immune subtypes among adults with sepsis in Uganda

Critical Care, 2022
Background The global burden of sepsis is concentrated in sub-Saharan Africa, where severe infections disproportionately affect young, HIV-infected adults and high-burden pathogens are unique. In this context, poor understanding of sepsis immunopathology
Matthew J. Cummings, Barnabas Bakamutumaho, Adam Price, Nicholas Owor, John Kayiwa, Joyce Namulondo, Timothy Byaruhanga, Moses Muwanga, Christopher Nsereko, Stephen Sameroff, Rafal Tokarz, Wai Wong, Shivang S. Shah, Michelle H. Larsen, W. Ian Lipkin, Julius J. Lutwama, Max R. O’Donnell   +16 more
doaj   +1 more source

Organoids in pediatric cancer research

FEBS Letters, EarlyView.
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley   +1 more source

Genomics‐led approach to drug testing in models of undifferentiated pleomorphic sarcoma

Molecular Oncology, EarlyView.
GA text Genomic data from undifferentiated pleomorphic sarcoma patients and preclinical models were used to inform a targeted drug screen. Selected compounds were tested in 2D and 3D cultures of UPS cell lines. A combination of trametinib and infigratinib was synergistic in the majority of UPS cell lines tested, which was further confirmed in an ex ...
Piotr J. Manasterski, Molly R. Danks, John P. Thomson, Morwenna Muir, Martin Lee, John C. Dawson, Ana T. Amaral, Juan Diaz‐Martin, David S. Moura, Javier Martin‐Broto, Ali Alsaadi, Donald M. Salter, Ailsa J. Oswald, Graeme Grimes, Larry Hayward, Ted R. Hupp, Karen Sisley, Paul H. Huang, Neil O. Carragher, Valerie G. Brunton   +19 more
wiley   +1 more source