Results 41 to 50 of about 153,654 (268)

A bioinformatics approach to microRNA-sequencing analysis

Osteoarthritis and Cartilage Open, 2021
The rapid expansion of Next Generation Sequencing (NGS) data availability has made exploration of appropriate bioinformatics analysis pipelines a timely issue.
Pratibha Potla, Shabana Amanda Ali, Mohit Kapoor   +2 more
doaj   +1 more source

Intervention of next-generation sequencing in diagnosis of Alzheimer’s disease: challenges and future prospects [PDF]

Dementia & Neuropsychologia, 2023
Clinical diagnosis of several neurodegenerative disorders based on clinical phenotype is challenging due to its heterogeneous nature and overlapping disease manifestations.
Tijimol Chandy
doaj   +1 more source

Genetic, Clinicopathological, and Radiological Features of Intrahepatic Cholangiocarcinoma with Ductal Plate Malformation Pattern

Gut and Liver, 2022
Background/Aims: Intrahepatic cholangiocarcinoma (iCCA) with a ductal plate malformation (DPM) pattern is a recently recognized rare variant. The genomic profile of iCCA with DPM pattern needs to be elucidated.
Taek Chung, Hyungjin Rhee, Hyo Sup Shim, Jeong Eun Yoo, Gi Hong Choi, Haeryoung Kim, Young Nyun Park   +6 more
doaj   +1 more source

Next-Generation Sequencing Reveals Differentially Expressed Genes and Pathways in Urethral Cancer: Exploring A Poorly Understood Malignancy [PDF]

Journal of Urologic Oncology
Purpose Primary urethral cancer (PUC) is an uncommon malignancy with scarce diagnostic and treatment options, resulting in a limited understanding of its genetic foundation. This exploratory study compares gene expression profiles between urethral cancer
Nickolas Kinachtchouk, Samantha Freeman, Rachael Gotlieb, Kailey Hooper, Travis Sullivan, Eric Burks, Kimberly Christ, Alex Vanni   +7 more
doaj   +1 more source

Chromosomal analyses of human giant diploid oocytes by next‐generation sequencing

Reproductive Medicine and Biology, 2021
Purpose Although giant oocytes (GOs) having about twice cytoplasmic volume compared with general oocytes in mammals including the human are rarely recovered, it is thought that GOs have potentially chromosomal abnormalities.
Hiroomi Kawano, Naoki Yamashita, Junya Ito, Naomi Kashiwazaki   +3 more
doaj   +1 more source

Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer

FEBS Letters, EarlyView.
Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani, Noemi E. Nwosu, Viktoria Gocke, Müge Kasanmascheff, Hannes Mutschler   +4 more
wiley   +1 more source

Multidimensional analysis of the host response reveals prognostic and pathogen-driven immune subtypes among adults with sepsis in Uganda

Critical Care, 2022
Background The global burden of sepsis is concentrated in sub-Saharan Africa, where severe infections disproportionately affect young, HIV-infected adults and high-burden pathogens are unique. In this context, poor understanding of sepsis immunopathology
Matthew J. Cummings, Barnabas Bakamutumaho, Adam Price, Nicholas Owor, John Kayiwa, Joyce Namulondo, Timothy Byaruhanga, Moses Muwanga, Christopher Nsereko, Stephen Sameroff, Rafal Tokarz, Wai Wong, Shivang S. Shah, Michelle H. Larsen, W. Ian Lipkin, Julius J. Lutwama, Max R. O’Donnell   +16 more
doaj   +1 more source

Cell wall target fragment discovery using a low‐cost, minimal fragment library

FEBS Letters, EarlyView.
LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan, Mathew Stanley, Olawale Raimi, Andrew T. Ferenbach, Helge C Dorfmueller, Daan M. F. van Aalten   +5 more
wiley   +1 more source

Detection of Persistent Viruses by High-Throughput Sequencing in Tomato and Pepper from Panama: Phylogenetic and Evolutionary Studies

Plants, 2021
High-throughput sequencing from symptomatic tomato and pepper plants collected in Panama rendered the complete genome of the southern tomato virus (isolate STV_Panama) and bell pepper endornavirus (isolate BPEV_Panama), and almost-complete genomes of ...
Luis Galipienso, Laura Elvira-González, Leonardo Velasco, José Ángel Herrera-Vásquez, Luis Rubio   +4 more
doaj   +1 more source

Liquid biopsy epigenetics: establishing a molecular profile based on cell‐free DNA

Molecular Oncology, EarlyView.
Cell‐free DNA (cfDNA) fragments in plasma from cancer patients carry epigenetic signatures reflecting their cells of origin. These epigenetic features include DNA methylation, nucleosome modifications, and variations in fragmentation. This review describes the biological properties of each feature and explores optimal strategies for harnessing cfDNA ...
Christoffer Trier Maansson, Anders Lade Nielsen, Boe Sandahl Sorensen   +2 more
wiley   +1 more source