Results 51 to 60 of about 229,759 (254)

Metagenomics by next-generation sequencing (mNGS) in the etiological characterization of neonatal and pediatric sepsis: A systematic review

Frontiers in Pediatrics, 2023
IntroductionPediatric and neonatal sepsis is one of the main causes of mortality and morbidity in these age groups. Accurate and early etiological identification is essential for guiding antibiotic treatment, improving survival, and reducing ...
Sergio Agudelo-Pérez, Jaime Fernández-Sarmiento, Jaime Fernández-Sarmiento, Diana Rivera León, Ronald Guillermo Peláez   +4 more
doaj   +1 more source

Lévy statistics in coding and non-coding nucleotide sequences [PDF]

, 2001
We propose a new method of statistical analysis of nucleotide sequences yielding the true scaling without requiring any form of de-trending. With the help of artificial sequences that are proved to be statistically equivalent to the real DNA sequences we find that power-law correlations are present in both coding and non-coding sequences, in accordance
arxiv   +1 more source

Covalent On‐Cell Conjugation of Biomaterials Through Oxidative Phenolic Coupling Regulates Stem Cell Fate via Intracellular Biophysical Programming

Advanced Functional Materials, EarlyView.
On‐cell covalent crosslinking of hydrogel matrix to the membrane of a single cell using droplet microfluidics enables mechanotransduction and influences stem cell fate. This newly established approach influences stem cell fate, independent of YAP/TAZ mechanotransduction and non‐covalent reversible binding ligands yet by various intracellular ...
Castro Johnbosco, Malin Becker, Niels Willemen, Marieke Meteling, Kannan Govindaraj, Tom Kamperman, Jeroen Leijten   +6 more
wiley   +1 more source

LinkedSV for detection of mosaic structural variants from linked-read exome and genome sequencing data

Nature Communications, 2019
Compared to single nucleotide variants and short indels, structural variants (SVs) are often more challenging to detect using high-throughput sequencing based methods. Here, the authors develop LinkedSV, a computational tool for SV detection using linked-
Li Fang, Charlly Kao, Michael V. Gonzalez, Fernanda A. Mafra, Renata Pellegrino da Silva, Mingyao Li, Sören-Sebastian Wenzel, Katharina Wimmer, Hakon Hakonarson, Kai Wang   +9 more
doaj   +1 more source

Effects of Somatic Mutations Are Associated with SNP in the Progression of Individual Acute Myeloid Leukemia Patient: The Two-Hit Theory Explains Inherited Predisposition to Pathogenesis [PDF]

Genomics & Informatics, 2013
This study evaluated the effects of somatic mutations and single nucleotide polymorphisms (SNPs) on disease progression and tried to verify the two-hit theory in cancer pathogenesis.
Soyoung Park, Youngil Koh, Sung-Soo Yoon
doaj   +1 more source

Direct-Coupling Analysis of nucleotide coevolution facilitates RNA secondary and tertiary structure prediction [PDF]

Nucl. Acids Res. (2015) doi: 10.1093/nar/gkv932, First published online: September 29, 2015, 2015
Despite the biological importance of non-coding RNA, their structural characterization remains challenging. Making use of the rapidly growing sequence databases, we analyze nucleotide coevolution across homologous sequences via Direct-Coupling Analysis to detect nucleotide-nucleotide contacts.
arxiv   +1 more source

Chiral Engineered Biomaterials: New Frontiers in Cellular Fate Regulation for Regenerative Medicine

Advanced Functional Materials, EarlyView.
Chiral engineered biomaterials can selectively influence cell behaviors in regenerative medicine. This review covers chiral engineered biomaterials in terms of their fabrication methods, cellular response mechanisms, and applications in directing stem cell differentiation and tissue function.
Yuwen Wang, Xin Zhang, Denghui Xie, Chunying Chen, Zhifeng Huang, Zhong Alan Li   +5 more
wiley   +1 more source

Genotyping by PCR and High-Throughput Sequencing of Commercial Probiotic Products Reveals Composition Biases.

Frontiers in Microbiology, 2016
Recent advances in microbiome research have brought renewed focus on beneficial bacteria, many of which are available in food and dietary supplements. Although probiotics have historically been defined as microorganisms that convey health benefits when ...
Wesley Morovic, Ashley A. Hibberd, Bryan Zabel, Rodolphe Barrangou, Buffy Stahl   +4 more
doaj   +1 more source

Porous Silicon Nanoneedles Efficiently Deliver Adenine Base Editor to Correct a Recurrent Pathogenic COL7A1 Variant in Recessive Dystrophic Epidermolysis Bullosa

Advanced Materials, EarlyView.
Base editing using nanoneedles efficiently corrects pathogenic variants in the type VII collagen gene of primary fibroblasts from patients with recessive dystrophic epidermolysis bullosa. Nanoneedle editing induces minimal and reversible transcriptional perturbations while restoring the production and secretion of functional type VII collagen.
Salman Ahmad Mustfa, Marija Dimitrievska, Cong Wang, Chenlei Gu, Ningjia Sun, Katarzyna Romańczuk, Pawel Karpinski, Łukasz Łaczmański, John A. McGrath, Joanna Jacków‐Malinowska, Ciro Chiappini   +10 more
wiley   +1 more source

Exploiting the Unique Properties of Lanthanide Complexes as FRET Probes: from Quantitation to Protein Dynamics

Analysis &Sensing, Volume 3, Issue 1, January 2023., 2023
Lanthanide complexes are ideal donors for FRET (Förster resonance energy transfer) assays with a variety of applications from easy‐to‐use quantitation of proteins to sophisticated experiments giving real‐time information on the movement of proteins. This Review article introduces the principles of using such assays before surveying the full scope of ...
Simon Wheeler, Stephen J. Butler
wiley   +1 more source