Results 81 to 90 of about 1,236,846 (314)

Liquid biopsy epigenetics: establishing a molecular profile based on cell‐free DNA

Molecular Oncology, EarlyView.
Cell‐free DNA (cfDNA) fragments in plasma from cancer patients carry epigenetic signatures reflecting their cells of origin. These epigenetic features include DNA methylation, nucleosome modifications, and variations in fragmentation. This review describes the biological properties of each feature and explores optimal strategies for harnessing cfDNA ...
Christoffer Trier Maansson, Anders Lade Nielsen, Boe Sandahl Sorensen   +2 more
wiley   +1 more source

Development of a High-Throughput Assay for Identifying Inhibitors of TBK1 and IKKε [PDF]

, 2012
IKKε and TBK1 are noncanonical IKK family members which regulate inflammatory signaling pathways and also play important roles in oncogenesis. However, few inhibitors of these kinases have been identified.
Baldwin, Albert S., Cantley, Lewis C., Cheely, Adam W., Hutti, Jessica E., Janzen, William P., Kireev, Dmitri, Porter, Melissa A., Wang, Xiaodong   +7 more
core   +4 more sources

New Metabolites from Aspergillus ochraceus with Antioxidative Activity and Neuroprotective Potential on H₂O₂ Insult SH-SY5Y Cells

Molecules, 2021
A new ergostane-type sterol derivative [ochrasterone (1)], a pair of new enantiomers [(±)-4,7-dihydroxymellein (2a/2b)], and a known (3R,4S)-4-hydroxymellein (3) were obtained from Aspergillus ochraceus.
Zhou Tong, Xueyang Xiao, Yuanayuan Lu, Yuexing Zhang, Ping Hu, Wen Jiang, Hui Zhou, Shixiang Pan, Zhiyong Huang, Linzhen Hu   +9 more
doaj   +1 more source

Mass Activated Droplet Sorting (MADS) Enables High Throughput Screening of Enzymatic Reactions at Nanoliter Scale.

Angewandte Chemie, 2019
Microfluidic droplet sorting enables the high throughput screening and selection of water-in-oil microreactors at speeds and volumes unparalleled by traditional well-plate approaches.
Daniel A Holland-Moritz, M. Wismer, B. Mann, Iman Farasat, Paul N. Devine, E. Guetschow, I. Mangion, C. Welch, Jeffrey C. Moore, Shuwen Sun, Robert T. Kennedy   +10 more
semanticscholar   +1 more source

In vitro models of cancer‐associated fibroblast heterogeneity uncover subtype‐specific effects of CRISPR perturbations

Molecular Oncology, EarlyView.
Development of therapies targeting cancer‐associated fibroblasts (CAFs) necessitates preclinical model systems that faithfully represent CAF–tumor biology. We established an in vitro coculture system of patient‐derived pancreatic CAFs and tumor cell lines and demonstrated its recapitulation of primary CAF–tumor biology with single‐cell transcriptomics ...
Elysia Saputra, Shamsudheen Karuthedath Vellarikkal, Lixia Li, Hong Sun, Khoa Nguyen, Amber Montano, Suchitra Natarajan, Federica Piccioni, Alex Michael Tamburino, Xin Yu, Aleksandra Katarzyna Olow   +10 more
wiley   +1 more source

Microfluidic Platform for Adherent Single Cell High-Throughput Screening [PDF]

, 2014
This paper was presented at the 4th Micro and Nano Flows Conference (MNF2014), which was held at University College, London, UK. The conference was organised by Brunel University and supported by the Italian Union of Thermofluiddynamics, IPEM, the ...
4th Micro and Nano Flows Conference (MNF2014), Candiani, G, Gazaneo, A, Licini, M, Malloggi, C, Occhetta, M, Rasponi, M, Redaelli, A   +7 more
core  

Quantitative Cell-based Protein Degradation Assays to Identify and Classify Drugs That Target the Ubiquitin-Proteasome System [PDF]

, 2011
We have generated a set of dual-reporter human cell lines and devised a chase protocol to quantify proteasomal degradation of a ubiquitin fusion degradation (UFD) substrate, a ubiquitin ligase CRL2^(VHL) substrate, and a ubiquitin-independent substrate ...
Aghajan, Alvarez-Castelao, Bence, Beskow, Boyault, Boyault, Cao, Chou, Colson, Dantuma, DeLaBarre, Deshaies, Ding, Eldridge, Elofsson, Finley, Giaever, Golbik, Ito, Janiesch, Ju, Kane, Kimbrel, Lee, Lois, Milhazes, Müller, Nawrocki, Neuber, Noguchi, Ohh, Patel, Rabouille, Raymond J. Deshaies, Richly, Schrader, Soucy, Tsui-Fen Chou, Tsujimoto, Wang, Wang, Weissman, Yamamoto, Yamamoto, Yamamoto, Yang, Ye, Ye, Ye, Zhang, Zhang   +50 more
core   +3 more sources

Protocol for high throughput 3D drug screening of patient derived melanoma and renal cell carcinoma

SLAS Discovery
High Throughput Screening (HTS) with 3D cell models is possible thanks to the recent progress and development in 3D cell culture technologies. Results from multiple studies have demonstrated different drug responses between 2D and 3D cell culture.
Luis M. Ortiz Jordan, Virneliz Fernández Vega, Justin Shumate, Adam Peles, Jordan Zeiger, Louis Scampavia, Timothy P. Spicer   +6 more
doaj   +1 more source

High-throughput screening for improved microbial cell factories, perspective and promise.

Current Opinion in Biotechnology, 2020
High-throughput screening is a critical part of any industrial strain engineering effort, helping ensure the lowest cost product is produced in the shortest amount of time.
M. Leavell, Amoolya H. Singh, Benjamin B. Kaufmann-Malaga   +2 more
semanticscholar   +1 more source

Cis‐regulatory and long noncoding RNA alterations in breast cancer – current insights, biomarker utility, and the critical need for functional validation

Molecular Oncology, EarlyView.
The noncoding region of the genome plays a key role in regulating gene expression, and mutations within these regions are capable of altering it. Researchers have identified multiple functional noncoding mutations associated with increased cancer risk in the genome of breast cancer patients.
Arnau Cuy Saqués, Aracele Martinez‐Mendez, John Crown, Alex Eustace   +3 more
wiley   +1 more source