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Cell-Based Assays for High-Throughput Screening
Molecular Biotechnology, 2010Cell-based assays represent approximately half of all high-throughput screens currently performed. Here, we review in brief the history and status of high-throughput screening (HTS), and summarize some of the challenges and benefits associated with the use of cell-based assays in HTS.
W Frank An +2 more
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Complex phenotypic assays in high-throughput screening
Current Opinion in Chemical Biology, 2004High-throughput screening (HTS), systematically testing thousands of small molecules to find candidates for lead optimization, primarily involves exposure of purified proteins to arrayed collections of small molecules. More complex phenotypic assays, such as cell-based or whole-organism assays, traditionally have flanked HTS, preceding it to validate ...
Paul A Clemons
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Acetylcholinesterase Inhibition Assays for High-Throughput Screening
2022Acetylcholinesterase (AChE) hydrolyzes acetylcholine (ACh), a vital neurotransmitter that regulates muscle movement and brain function, including memory, attention, and learning. Inhibition of AChE activity can cause a variety of adverse health effects and toxicity. Identifying AChE inhibitors quickly and efficiently warrants developing AChE inhibition
Shuaizhang, Li +4 more
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Bioluminescent Assays for High-Throughput Screening
ASSAY and Drug Development Technologies, 2007In the development of high throughput screening (HTS) as a central paradigm of drug discovery, fluorescence has generally been adopted as the favored methodology. Nevertheless, luminescence has maintained a prominent position among certain assay formats, most notably genetic reporters.
Frank, Fan, Keith V, Wood
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Enzyme assays for high-throughput screening
Current Opinion in Biotechnology, 2004Assaying enzyme-catalyzed transformations in high-throughput is crucial to enzyme discovery, enzyme engineering and the drug discovery process. In enzyme assays, catalytic activity is detected using labelled substrates or indirect sensor systems that produce a detectable spectroscopic signal upon reaction.
Goddard JP, Reymond JL
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Design and Implementation of High Throughput Screening Assays
Molecular Biotechnology, 2003HTS is at the core of the drug-discovery process, and so it is critical to design and implement HTS assays in a comprehensive fashion involving scientists from the disciplines of biology, chemistry, engineering, and informatics. This requires careful analysis of many variables, starting with the choice of assay target and ending with the discovery of ...
Ricardo, Macarrón, Robert P, Hertzberg
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High-throughput screening: advances in assay technologies
Current Opinion in Chemical Biology, 1997Both isotopic and nonisotopic assay methodologies are employed in high-throughput screening for drug discovery. Recent advances in cell-based and in vitro biochemical assays will be reviewed, with special emphasis on detection technologies amenable to automated 'mix and read' procedures in high-throughput screening.
G S, Sittampalam, S D, Kahl, W P, Janzen
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New assay technologies for high-throughput screening
Current Opinion in Chemical Biology, 1998The use of high-throughput screening for early stage drug discovery imposes several constraints on the format of assays for therapeutic targets of interest. Homogeneous cell-free assays based on energy transfer, fluorescence polarization spectroscopy or fluorescence correlation spectroscopy provide the sensitivity, ease, speed and resistance to ...
L, Silverman, R, Campbell, J R, Broach
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Scintillation Proximity Assays in High-Throughput Screening
ASSAY and Drug Development Technologies, 2008Scintillation proximity assays (SPAs) have become a powerful tool for high-throughput screening (HTS) because they can measure the activity and binding of very diverse classes of drug targets. By applying the basic principles of ligand-receptor binding and enzyme kinetics, it is possible to build a large variety of miniaturized, high-throughput assays ...
J Fraser, Glickman +2 more
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