Results 211 to 220 of about 123,864 (254)

Intracellular detection assays for high-throughput screening

Current Opinion in Biotechnology, 1998
New optical assay methods promise to accelerate the use of living cells in screens for drug discovery. Most of these methods employ either fluorescent or luminescent read-outs and allow cell-based assays for most targets, including receptors, ion channels and intracellular enzymes. Furthermore, genetically encoded probes offer the possibility of custom-
J E, González, P A, Negulescu
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High-Throughput Screening Assay for Helicase Enzymes

Analytical Biochemistry, 1998
We have developed a novel 96-well microtiter plate high-throughput screening filtration assay for the detection of helicase activity. In this paper we present data for detection of helicase activity of the UL5/8/52 helicase-primase complex from herpes simplex virus 1 (HSV1).
M, Sivaraja, H, Giordano, M G, Peterson
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High-Throughput Screening Assays for Lipolytic Enzymes

2017
Screening is defined as the identification of hits within a large library of variants of an enzyme or protein with a predefined property. In theory, each variant present in the respective library needs to be assayed; however, to save time and consumables, many screening regimes involve a primary round to identify clones producing active enzymes.
Alexander, Fulton, Marc R, Hayes, Ulrich, Schwaneberg, Jörg, Pietruszka, Karl-Erich, Jaeger   +4 more
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Complement C5a Receptor Assay for High Throughput Screening

Journal of Receptor Research, 1991
The complement C5a receptor on U937 cells, a human histiocytic lymphoma cell line, stimulated with dibutyryl-cAMP have been stabilized for at least 3 months at a dilute, ready to use concentration. [125I]-Bolton Hunter labeled C5a, (recombinant, human) has been prepared by reverse phase HPLC to 2200 Ci/mmol. Using a filtration binding assay the Kd from
S R, Harris, R K, Garlick, J J, Miller, H N, Harney, P J, Monroe   +4 more
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Enzyme Assay Design for High-Throughput Screening

2009
Enzymes continue to be a major drug target class for the pharmaceutical industry with high-throughput screening the approach of choice for identifying initial active chemical compounds. The development of fluorescent- or absorbance-based readouts typically remains the formats of choice for enzyme screens and a wealth of experience from both industry ...
Kevin P, Williams, John E, Scott
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Development of a Thyroperoxidase Inhibition Assay for High-Throughput Screening

Chemical Research in Toxicology, 2014
High-throughput screening (HTPS) assays to detect inhibitors of thyroperoxidase (TPO), the enzymatic catalyst for thyroid hormone (TH) synthesis, are not currently available. Herein, we describe the development of a HTPS TPO inhibition assay. Rat thyroid microsomes and a fluorescent peroxidase substrate, Amplex UltraRed (AUR), were employed in an end ...
Katie B, Paul, Joan M, Hedge, Daniel M, Rotroff, Michael W, Hornung, Kevin M, Crofton, Steven O, Simmons   +5 more
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In Vitro High-Throughput Screening Assay for Modulators of Transcription

Analytical Biochemistry, 2002
We developed a 96-well microtiter-plate high-throughput screening (HTS) assay for the detection of modulators of transcription. This HTS assay consists of three steps: (1) the in vitro transcription reaction; (2) modification and hybridization of RNA products; and (3) washing and quantification.
León F, García-Martínez, Graham K, Bilter, Jun, Wu, Joe, O'Neill, Miguel S, Barbosa, Robert, Kovelman   +5 more
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Design and Implementation of High-Throughput Screening Assays

2016
HTS remains at the core of the drug discovery process, and so it is critical to design and implement HTS assays in a comprehensive fashion involving scientists from the disciplines of biology, chemistry, engineering, and informatics. This requires careful consideration of many options and variables, starting with the choice of screening strategy and ...
David J, Powell, Robert P, Hertzberg, Ricardo, Macarrόn   +2 more
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A miniaturized high-throughput screening assay for fucosyltransferase VII

Analytical Biochemistry, 2008
Fucosyltransferase VII (FucTVII) is a very promising drug target for treatment of inflammatory skin diseases. Its activity is required for synthesis of the sialyl-Lewis X glycoepitopes on the E- and P-selectin ligands, necessary for lymphocyte migration into the skin.
Oliver von, Ahsen, Ulrike, Voigtmann, Monika, Klotz, Nikolay, Nifantiev, Arndt, Schottelius, Alexander, Ernst, Beate, Müller-Tiemann, Karsten, Parczyk   +7 more
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High-Throughput Screening Assay for Biological Hydrogen Production

Analytical Chemistry, 2008
This paper describes a screening assay, compatible with high-throughput bioprospecting or molecular biology methods, for assessing biological hydrogen (H2) production. While the assay is adaptable to various physical configurations, we describe its use in a 96-well, microtiter plate format with a lower plate containing H2-producing cyanobacteria ...
Paul S, Schrader, Elizabeth H, Burrows, Roger L, Ely   +2 more
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