Results 231 to 240 of about 16,128,167 (280)
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A fluorescence-based glycosyltransferase assay for high-throughput screening
Bioorganic and Medicinal Chemistry, 2014Glycosyltransferases catalyze the transfer of a monosaccharide unit from a nucleotide or lipid sugar donor to polysaccharides, lipids, and proteins in a stereospecific manner. Considerable effort has been invested in engineering glycosyltransferases to diversify sugar-containing drugs. An important requirement for glycosyltransferase engineering is the
Min Sik Eom, Min Su Han, Hyun Soo Lee
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Acetylcholinesterase Inhibition Assays for High-Throughput Screening
2022Acetylcholinesterase (AChE) hydrolyzes acetylcholine (ACh), a vital neurotransmitter that regulates muscle movement and brain function, including memory, attention, and learning. Inhibition of AChE activity can cause a variety of adverse health effects and toxicity. Identifying AChE inhibitors quickly and efficiently warrants developing AChE inhibition
Shuaizhang, Li +4 more
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Bioluminescent Assays for High-Throughput Screening
ASSAY and Drug Development Technologies, 2007In the development of high throughput screening (HTS) as a central paradigm of drug discovery, fluorescence has generally been adopted as the favored methodology. Nevertheless, luminescence has maintained a prominent position among certain assay formats, most notably genetic reporters.
Frank, Fan, Keith V, Wood
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Design and Implementation of High Throughput Screening Assays
Molecular Biotechnology, 2003HTS is at the core of the drug-discovery process, and so it is critical to design and implement HTS assays in a comprehensive fashion involving scientists from the disciplines of biology, chemistry, engineering, and informatics. This requires careful analysis of many variables, starting with the choice of assay target and ending with the discovery of ...
Ricardo, Macarrón, Robert P, Hertzberg
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Intracellular detection assays for high-throughput screening
Current Opinion in Biotechnology, 1998New optical assay methods promise to accelerate the use of living cells in screens for drug discovery. Most of these methods employ either fluorescent or luminescent read-outs and allow cell-based assays for most targets, including receptors, ion channels and intracellular enzymes. Furthermore, genetically encoded probes offer the possibility of custom-
J E, González, P A, Negulescu
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High-Throughput Screening Assay for Helicase Enzymes
Analytical Biochemistry, 1998We have developed a novel 96-well microtiter plate high-throughput screening filtration assay for the detection of helicase activity. In this paper we present data for detection of helicase activity of the UL5/8/52 helicase-primase complex from herpes simplex virus 1 (HSV1).
M, Sivaraja, H, Giordano, M G, Peterson
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High-Throughput Screening Assays for Lipolytic Enzymes
2017Screening is defined as the identification of hits within a large library of variants of an enzyme or protein with a predefined property. In theory, each variant present in the respective library needs to be assayed; however, to save time and consumables, many screening regimes involve a primary round to identify clones producing active enzymes.
Alexander, Fulton +4 more
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Enzyme Assay Design for High-Throughput Screening
2009Enzymes continue to be a major drug target class for the pharmaceutical industry with high-throughput screening the approach of choice for identifying initial active chemical compounds. The development of fluorescent- or absorbance-based readouts typically remains the formats of choice for enzyme screens and a wealth of experience from both industry ...
Kevin P, Williams, John E, Scott
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In Vitro High-Throughput Screening Assay for Modulators of Transcription
Analytical Biochemistry, 2002We developed a 96-well microtiter-plate high-throughput screening (HTS) assay for the detection of modulators of transcription. This HTS assay consists of three steps: (1) the in vitro transcription reaction; (2) modification and hybridization of RNA products; and (3) washing and quantification.
León F, García-Martínez +5 more
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Design and Implementation of High-Throughput Screening Assays
2016HTS remains at the core of the drug discovery process, and so it is critical to design and implement HTS assays in a comprehensive fashion involving scientists from the disciplines of biology, chemistry, engineering, and informatics. This requires careful consideration of many options and variables, starting with the choice of screening strategy and ...
David J, Powell +2 more
openaire +2 more sources