Results 241 to 250 of about 16,128,167 (280)

A miniaturized high-throughput screening assay for fucosyltransferase VII

Analytical Biochemistry, 2008
Fucosyltransferase VII (FucTVII) is a very promising drug target for treatment of inflammatory skin diseases. Its activity is required for synthesis of the sialyl-Lewis X glycoepitopes on the E- and P-selectin ligands, necessary for lymphocyte migration into the skin.
Oliver von, Ahsen, Ulrike, Voigtmann, Monika, Klotz, Nikolay, Nifantiev, Arndt, Schottelius, Alexander, Ernst, Beate, Müller-Tiemann, Karsten, Parczyk   +7 more
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High-Throughput Screening Assay for Biological Hydrogen Production

Analytical Chemistry, 2008
This paper describes a screening assay, compatible with high-throughput bioprospecting or molecular biology methods, for assessing biological hydrogen (H2) production. While the assay is adaptable to various physical configurations, we describe its use in a 96-well, microtiter plate format with a lower plate containing H2-producing cyanobacteria ...
Paul S, Schrader, Elizabeth H, Burrows, Roger L, Ely   +2 more
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A Colloidal Stability Assay Suitable for High-Throughput Screening

Analytical Chemistry, 2016
A library of 32 polystyrene copolymer latexes, with diameters ranging between 53 and 387 nm, was used to develop and demonstrate a high-throughput assay using a 96-well microplate platform to measure critical coagulation concentrations, a measure of colloidal stability.
Carla, Abarca, M Monsur, Ali, Songtao, Yang, Xiaofei, Dong, Robert H, Pelton   +4 more
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Complex phenotypic assays in high-throughput screening

Current Opinion in Chemical Biology, 2004
High-throughput screening (HTS), systematically testing thousands of small molecules to find candidates for lead optimization, primarily involves exposure of purified proteins to arrayed collections of small molecules. More complex phenotypic assays, such as cell-based or whole-organism assays, traditionally have flanked HTS, preceding it to validate ...
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Introduction: Cell-Based Assays for High-Throughput Screening

2009
Cell-based assays represent approximately half of all high-throughput screens (HTS) currently performed. Here we review the history and status of HTS, and summarize some of the challenges and benefits associated with the use of cell-based assays in HTS, drawing upon themes that will reemerge in subsequent chapters in this book.
W Frank, An, Nicola J, Tolliday
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Fluorescence Assays for High-Throughput Screening of Protein Kinases

Combinatorial Chemistry & High Throughput Screening, 2003
Protein kinases comprise one of the most important group of targets for drug discovery research today. Methods to identify novel kinase inhibitors by high-throughput screening have evolved rapidly in recent years. An important aspect is the availability of fluorescent probes that can be applied in a homogeneous, or mix-and-measure, assay format.
G J R, Zaman, A, Garritsen, Th, de Boer, C A A, van Boeckel   +3 more
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High Throughput Receptor Screening Assays

2017
The recent technical advances in functional expression of olfactory receptors (OR s) make full deorphanization of human OR repertoire a realistic objective. Such a global knowledge of the precise mechanisms of odorant/receptor pairings will represent a crucial step for the development of an accurate model of how human nose perceives its chemical ...
Alex Veithen, Magali Philippeau, Pierre Chatelain   +2 more
openaire   +1 more source

Complement C5a Receptor Assay for High Throughput Screening

Journal of Receptor Research, 1991
The complement C5a receptor on U937 cells, a human histiocytic lymphoma cell line, stimulated with dibutyryl-cAMP have been stabilized for at least 3 months at a dilute, ready to use concentration. [125I]-Bolton Hunter labeled C5a, (recombinant, human) has been prepared by reverse phase HPLC to 2200 Ci/mmol. Using a filtration binding assay the Kd from
S R, Harris, R K, Garlick, J J, Miller, H N, Harney, P J, Monroe   +4 more
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Development of a Thyroperoxidase Inhibition Assay for High-Throughput Screening

Chemical Research in Toxicology, 2014
High-throughput screening (HTPS) assays to detect inhibitors of thyroperoxidase (TPO), the enzymatic catalyst for thyroid hormone (TH) synthesis, are not currently available. Herein, we describe the development of a HTPS TPO inhibition assay. Rat thyroid microsomes and a fluorescent peroxidase substrate, Amplex UltraRed (AUR), were employed in an end ...
Katie B, Paul, Joan M, Hedge, Daniel M, Rotroff, Michael W, Hornung, Kevin M, Crofton, Steven O, Simmons   +5 more
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A high-throughput screening assay for hydroxynitrile lyase activity

Chemical Communications, 2006
A high-throughput screening assay for hydroxynitrile lyase activity accepting a wide range of HNL-substrates is presented, which is useful either for enzyme fingerprinting or screening of huge variant libraries generated in metagenome or directed evolution approaches.
Andexer, Jennifer, Guterl, Jan-Karl, Pohl, Martina, Eggert, Thorsten   +3 more
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