Results 221 to 230 of about 123,864 (254)

A Colloidal Stability Assay Suitable for High-Throughput Screening

Analytical Chemistry, 2016
A library of 32 polystyrene copolymer latexes, with diameters ranging between 53 and 387 nm, was used to develop and demonstrate a high-throughput assay using a 96-well microplate platform to measure critical coagulation concentrations, a measure of colloidal stability.
Carla, Abarca, M Monsur, Ali, Songtao, Yang, Xiaofei, Dong, Robert H, Pelton   +4 more
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Introduction: Cell-Based Assays for High-Throughput Screening

2009
Cell-based assays represent approximately half of all high-throughput screens (HTS) currently performed. Here we review the history and status of HTS, and summarize some of the challenges and benefits associated with the use of cell-based assays in HTS, drawing upon themes that will reemerge in subsequent chapters in this book.
W Frank, An, Nicola J, Tolliday
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High Throughput Receptor Screening Assays

2017
The recent technical advances in functional expression of olfactory receptors (OR s) make full deorphanization of human OR repertoire a realistic objective. Such a global knowledge of the precise mechanisms of odorant/receptor pairings will represent a crucial step for the development of an accurate model of how human nose perceives its chemical ...
Alex Veithen, Magali Philippeau, Pierre Chatelain   +2 more
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High-throughput screening assays for the identification of chemical probes

Nature Chemical Biology, 2007
High-throughput screening (HTS) assays enable the testing of large numbers of chemical substances for activity in diverse areas of biology. The biological responses measured in HTS assays span isolated biochemical systems containing purified receptors or enzymes to signal transduction pathways and complex networks functioning in cellular environments ...
James, Inglese, Ronald L, Johnson, Anton, Simeonov, Menghang, Xia, Wei, Zheng, Christopher P, Austin, Douglas S, Auld   +6 more
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A high-throughput screening assay for hydroxynitrile lyase activity

Chemical Communications, 2006
A high-throughput screening assay for hydroxynitrile lyase activity accepting a wide range of HNL-substrates is presented, which is useful either for enzyme fingerprinting or screening of huge variant libraries generated in metagenome or directed evolution approaches.
Andexer, Jennifer, Guterl, Jan-Karl, Pohl, Martina, Eggert, Thorsten   +3 more
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Fluorescence Assays for High-Throughput Screening of Protein Kinases

Combinatorial Chemistry & High Throughput Screening, 2003
Protein kinases comprise one of the most important group of targets for drug discovery research today. Methods to identify novel kinase inhibitors by high-throughput screening have evolved rapidly in recent years. An important aspect is the availability of fluorescent probes that can be applied in a homogeneous, or mix-and-measure, assay format.
G J R, Zaman, A, Garritsen, Th, de Boer, C A A, van Boeckel   +3 more
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A fluorescence-based glycosyltransferase assay for high-throughput screening

Bioorganic & Medicinal Chemistry, 2014
Glycosyltransferases catalyze the transfer of a monosaccharide unit from a nucleotide or lipid sugar donor to polysaccharides, lipids, and proteins in a stereospecific manner. Considerable effort has been invested in engineering glycosyltransferases to diversify sugar-containing drugs. An important requirement for glycosyltransferase engineering is the
Jihye, Ryu, Min Sik, Eom, Wooseok, Ko, Min Su, Han, Hyun Soo, Lee   +4 more
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Phosphatase High-Throughput Screening Assay Design and Selection

2013
Phosphatases are a heterogeneous group of enzymes catalyzing dephosphorylation of diverse substrates ranging from small organic molecules to large phosphorylated multiprotein complexes. A wide variety of biochemical approaches for measuring phosphatase activity exists.
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Microplate assays for high-throughput screening.

Current opinion in drug discovery & development, 2009
Microplates containing an 8 x 12 array of 96-wells are the most prevalent format for screening compound libraries in high-throughput applications today. These plates are suitable for use in a wide variety of homogeneous and heterogeneous assays to measure binding interactions, enzyme activity and cell proliferation.
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A high throughput screening assay for human Thyroperoxidase inhibitors

Toxicology in Vitro
Rapid, human relevant assays are needed to assess potential hazards of the many chemicals in commerce. An assay of thyroid peroxidase (TPO) inhibition, using the substrate Amplex Ultra Red, was recently adapted for human TPO (AUR-hTPO). We tested a large number (788) of chemicals through this AUR-hTPO assay and compared performance with published ...
Hongyan, Dong, Katie Paul, Friedman, Alain, Filiatreault, Errol M, Thomson, Michael G, Wade   +4 more
openaire   +2 more sources