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Bioluminescent Assays for High-Throughput Screening
ASSAY and Drug Development Technologies, 2007In the development of high throughput screening (HTS) as a central paradigm of drug discovery, fluorescence has generally been adopted as the favored methodology. Nevertheless, luminescence has maintained a prominent position among certain assay formats, most notably genetic reporters.
Frank, Fan, Keith V, Wood
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Design and Implementation of High Throughput Screening Assays
Molecular Biotechnology, 2003HTS is at the core of the drug-discovery process, and so it is critical to design and implement HTS assays in a comprehensive fashion involving scientists from the disciplines of biology, chemistry, engineering, and informatics. This requires careful analysis of many variables, starting with the choice of assay target and ending with the discovery of ...
Ricardo, Macarrón, Robert P, Hertzberg
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High-throughput screening: advances in assay technologies
Current Opinion in Chemical Biology, 1997Both isotopic and nonisotopic assay methodologies are employed in high-throughput screening for drug discovery. Recent advances in cell-based and in vitro biochemical assays will be reviewed, with special emphasis on detection technologies amenable to automated 'mix and read' procedures in high-throughput screening.
G S, Sittampalam, S D, Kahl, W P, Janzen
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New assay technologies for high-throughput screening
Current Opinion in Chemical Biology, 1998The use of high-throughput screening for early stage drug discovery imposes several constraints on the format of assays for therapeutic targets of interest. Homogeneous cell-free assays based on energy transfer, fluorescence polarization spectroscopy or fluorescence correlation spectroscopy provide the sensitivity, ease, speed and resistance to ...
L, Silverman, R, Campbell, J R, Broach
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Intracellular detection assays for high-throughput screening
Current Opinion in Biotechnology, 1998New optical assay methods promise to accelerate the use of living cells in screens for drug discovery. Most of these methods employ either fluorescent or luminescent read-outs and allow cell-based assays for most targets, including receptors, ion channels and intracellular enzymes. Furthermore, genetically encoded probes offer the possibility of custom-
J E, González, P A, Negulescu
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High-Throughput Screening Assay for Helicase Enzymes
Analytical Biochemistry, 1998We have developed a novel 96-well microtiter plate high-throughput screening filtration assay for the detection of helicase activity. In this paper we present data for detection of helicase activity of the UL5/8/52 helicase-primase complex from herpes simplex virus 1 (HSV1).
M, Sivaraja, H, Giordano, M G, Peterson
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High-Throughput Screening Assays for Lipolytic Enzymes
2017Screening is defined as the identification of hits within a large library of variants of an enzyme or protein with a predefined property. In theory, each variant present in the respective library needs to be assayed; however, to save time and consumables, many screening regimes involve a primary round to identify clones producing active enzymes.
Alexander, Fulton +4 more
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Enzyme Assay Design for High-Throughput Screening
2009Enzymes continue to be a major drug target class for the pharmaceutical industry with high-throughput screening the approach of choice for identifying initial active chemical compounds. The development of fluorescent- or absorbance-based readouts typically remains the formats of choice for enzyme screens and a wealth of experience from both industry ...
Kevin P, Williams, John E, Scott
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Complement C5a Receptor Assay for High Throughput Screening
Journal of Receptor Research, 1991The complement C5a receptor on U937 cells, a human histiocytic lymphoma cell line, stimulated with dibutyryl-cAMP have been stabilized for at least 3 months at a dilute, ready to use concentration. [125I]-Bolton Hunter labeled C5a, (recombinant, human) has been prepared by reverse phase HPLC to 2200 Ci/mmol. Using a filtration binding assay the Kd from
S R, Harris +4 more
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Development of a Thyroperoxidase Inhibition Assay for High-Throughput Screening
Chemical Research in Toxicology, 2014High-throughput screening (HTPS) assays to detect inhibitors of thyroperoxidase (TPO), the enzymatic catalyst for thyroid hormone (TH) synthesis, are not currently available. Herein, we describe the development of a HTPS TPO inhibition assay. Rat thyroid microsomes and a fluorescent peroxidase substrate, Amplex UltraRed (AUR), were employed in an end ...
Katie B, Paul +5 more
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