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High-throughput screening: advances in assay technologies
Current Opinion in Chemical Biology, 1997Both isotopic and nonisotopic assay methodologies are employed in high-throughput screening for drug discovery. Recent advances in cell-based and in vitro biochemical assays will be reviewed, with special emphasis on detection technologies amenable to automated 'mix and read' procedures in high-throughput screening.
G S, Sittampalam, S D, Kahl, W P, Janzen
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New assay technologies for high-throughput screening
Current Opinion in Chemical Biology, 1998The use of high-throughput screening for early stage drug discovery imposes several constraints on the format of assays for therapeutic targets of interest. Homogeneous cell-free assays based on energy transfer, fluorescence polarization spectroscopy or fluorescence correlation spectroscopy provide the sensitivity, ease, speed and resistance to ...
L, Silverman, R, Campbell, J R, Broach
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Cell-Based Assays for High-Throughput Screening
Molecular Biotechnology, 2010Cell-based assays represent approximately half of all high-throughput screens currently performed. Here, we review in brief the history and status of high-throughput screening (HTS), and summarize some of the challenges and benefits associated with the use of cell-based assays in HTS.
W Frank, An, Nicola, Tolliday
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Intracellular detection assays for high-throughput screening
Current Opinion in Biotechnology, 1998New optical assay methods promise to accelerate the use of living cells in screens for drug discovery. Most of these methods employ either fluorescent or luminescent read-outs and allow cell-based assays for most targets, including receptors, ion channels and intracellular enzymes. Furthermore, genetically encoded probes offer the possibility of custom-
J E, González, P A, Negulescu
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High-Throughput Screening Assay for Helicase Enzymes
Analytical Biochemistry, 1998We have developed a novel 96-well microtiter plate high-throughput screening filtration assay for the detection of helicase activity. In this paper we present data for detection of helicase activity of the UL5/8/52 helicase-primase complex from herpes simplex virus 1 (HSV1).
M, Sivaraja, H, Giordano, M G, Peterson
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Scintillation Proximity Assays in High-Throughput Screening
ASSAY and Drug Development Technologies, 2008Scintillation proximity assays (SPAs) have become a powerful tool for high-throughput screening (HTS) because they can measure the activity and binding of very diverse classes of drug targets. By applying the basic principles of ligand-receptor binding and enzyme kinetics, it is possible to build a large variety of miniaturized, high-throughput assays ...
J Fraser, Glickman +2 more
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Design and Implementation of High Throughput Screening Assays
Molecular Biotechnology, 2003HTS is at the core of the drug-discovery process, and so it is critical to design and implement HTS assays in a comprehensive fashion involving scientists from the disciplines of biology, chemistry, engineering, and informatics. This requires careful analysis of many variables, starting with the choice of assay target and ending with the discovery of ...
Ricardo, Macarrón, Robert P, Hertzberg
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Enzyme Assay Design for High-Throughput Screening
2009Enzymes continue to be a major drug target class for the pharmaceutical industry with high-throughput screening the approach of choice for identifying initial active chemical compounds. The development of fluorescent- or absorbance-based readouts typically remains the formats of choice for enzyme screens and a wealth of experience from both industry ...
Kevin P, Williams, John E, Scott
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High Throughput Receptor Screening Assays
2017The recent technical advances in functional expression of olfactory receptors (OR s) make full deorphanization of human OR repertoire a realistic objective. Such a global knowledge of the precise mechanisms of odorant/receptor pairings will represent a crucial step for the development of an accurate model of how human nose perceives its chemical ...
Alex Veithen +2 more
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High-Throughput Screening Assays for Lipolytic Enzymes
2017Screening is defined as the identification of hits within a large library of variants of an enzyme or protein with a predefined property. In theory, each variant present in the respective library needs to be assayed; however, to save time and consumables, many screening regimes involve a primary round to identify clones producing active enzymes.
Alexander, Fulton +4 more
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