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A fluorescence-based glycosyltransferase assay for high-throughput screening
Bioorganic & Medicinal Chemistry, 2014Glycosyltransferases catalyze the transfer of a monosaccharide unit from a nucleotide or lipid sugar donor to polysaccharides, lipids, and proteins in a stereospecific manner. Considerable effort has been invested in engineering glycosyltransferases to diversify sugar-containing drugs. An important requirement for glycosyltransferase engineering is the
Jihye, Ryu +4 more
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A novel screening assay for hydroxynitrile lyases suitable for high-throughput screening
Journal of Biotechnology, 2007Hydroxynitrile lyases (Hnls) are important biocatalysts for the synthesis of optically pure cyanohydrins, which are used as precursors and building blocks for a wide range of high price fine chemicals. Although two Hnl enzymes, from the tropical rubber tree Hevea brasiliensis and from the almond tree Prunus amygdalus, are already used for large scale ...
B, Krammer +4 more
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A high throughput screening assay for human Thyroperoxidase inhibitors
Toxicology in VitroRapid, human relevant assays are needed to assess potential hazards of the many chemicals in commerce. An assay of thyroid peroxidase (TPO) inhibition, using the substrate Amplex Ultra Red, was recently adapted for human TPO (AUR-hTPO). We tested a large number (788) of chemicals through this AUR-hTPO assay and compared performance with published ...
Hongyan, Dong +4 more
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Phosphatase High-Throughput Screening Assay Design and Selection
2013Phosphatases are a heterogeneous group of enzymes catalyzing dephosphorylation of diverse substrates ranging from small organic molecules to large phosphorylated multiprotein complexes. A wide variety of biochemical approaches for measuring phosphatase activity exists.
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Microplate assays for high-throughput screening.
Current opinion in drug discovery & development, 2009Microplates containing an 8 x 12 array of 96-wells are the most prevalent format for screening compound libraries in high-throughput applications today. These plates are suitable for use in a wide variety of homogeneous and heterogeneous assays to measure binding interactions, enzyme activity and cell proliferation.
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