Results 261 to 270 of about 137,634 (294)

In Vitro High-Throughput Screening Assay for Modulators of Transcription

Analytical Biochemistry, 2002
We developed a 96-well microtiter-plate high-throughput screening (HTS) assay for the detection of modulators of transcription. This HTS assay consists of three steps: (1) the in vitro transcription reaction; (2) modification and hybridization of RNA products; and (3) washing and quantification.
León F, García-Martínez, Graham K, Bilter, Jun, Wu, Joe, O'Neill, Miguel S, Barbosa, Robert, Kovelman   +5 more
openaire   +2 more sources

Design and Implementation of High-Throughput Screening Assays

2016
HTS remains at the core of the drug discovery process, and so it is critical to design and implement HTS assays in a comprehensive fashion involving scientists from the disciplines of biology, chemistry, engineering, and informatics. This requires careful consideration of many options and variables, starting with the choice of screening strategy and ...
David J, Powell, Robert P, Hertzberg, Ricardo, Macarrόn   +2 more
openaire   +2 more sources

A Colloidal Stability Assay Suitable for High-Throughput Screening

Analytical Chemistry, 2016
A library of 32 polystyrene copolymer latexes, with diameters ranging between 53 and 387 nm, was used to develop and demonstrate a high-throughput assay using a 96-well microplate platform to measure critical coagulation concentrations, a measure of colloidal stability.
Carla, Abarca, M Monsur, Ali, Songtao, Yang, Xiaofei, Dong, Robert H, Pelton   +4 more
openaire   +2 more sources

A miniaturized high-throughput screening assay for fucosyltransferase VII

Analytical Biochemistry, 2008
Fucosyltransferase VII (FucTVII) is a very promising drug target for treatment of inflammatory skin diseases. Its activity is required for synthesis of the sialyl-Lewis X glycoepitopes on the E- and P-selectin ligands, necessary for lymphocyte migration into the skin.
Oliver von, Ahsen, Ulrike, Voigtmann, Monika, Klotz, Nikolay, Nifantiev, Arndt, Schottelius, Alexander, Ernst, Beate, Müller-Tiemann, Karsten, Parczyk   +7 more
openaire   +2 more sources

High-Throughput Screening Assay for Biological Hydrogen Production

Analytical Chemistry, 2008
This paper describes a screening assay, compatible with high-throughput bioprospecting or molecular biology methods, for assessing biological hydrogen (H2) production. While the assay is adaptable to various physical configurations, we describe its use in a 96-well, microtiter plate format with a lower plate containing H2-producing cyanobacteria ...
Paul S, Schrader, Elizabeth H, Burrows, Roger L, Ely   +2 more
openaire   +2 more sources

High-throughput screening assays for the identification of chemical probes

Nature Chemical Biology, 2007
High-throughput screening (HTS) assays enable the testing of large numbers of chemical substances for activity in diverse areas of biology. The biological responses measured in HTS assays span isolated biochemical systems containing purified receptors or enzymes to signal transduction pathways and complex networks functioning in cellular environments ...
James, Inglese, Ronald L, Johnson, Anton, Simeonov, Menghang, Xia, Wei, Zheng, Christopher P, Austin, Douglas S, Auld   +6 more
openaire   +2 more sources

Introduction: Cell-Based Assays for High-Throughput Screening

2009
Cell-based assays represent approximately half of all high-throughput screens (HTS) currently performed. Here we review the history and status of HTS, and summarize some of the challenges and benefits associated with the use of cell-based assays in HTS, drawing upon themes that will reemerge in subsequent chapters in this book.
W Frank, An, Nicola J, Tolliday
openaire   +2 more sources

High Throughput Receptor Screening Assays

2017
The recent technical advances in functional expression of olfactory receptors (OR s) make full deorphanization of human OR repertoire a realistic objective. Such a global knowledge of the precise mechanisms of odorant/receptor pairings will represent a crucial step for the development of an accurate model of how human nose perceives its chemical ...
Alex Veithen, Magali Philippeau, Pierre Chatelain   +2 more
openaire   +1 more source

A high-throughput screening assay for hydroxynitrile lyase activity

Chemical Communications, 2006
A high-throughput screening assay for hydroxynitrile lyase activity accepting a wide range of HNL-substrates is presented, which is useful either for enzyme fingerprinting or screening of huge variant libraries generated in metagenome or directed evolution approaches.
Andexer, Jennifer, Guterl, Jan-Karl, Pohl, Martina, Eggert, Thorsten   +3 more
openaire   +3 more sources

Fluorescence Assays for High-Throughput Screening of Protein Kinases

Combinatorial Chemistry & High Throughput Screening, 2003
Protein kinases comprise one of the most important group of targets for drug discovery research today. Methods to identify novel kinase inhibitors by high-throughput screening have evolved rapidly in recent years. An important aspect is the availability of fluorescent probes that can be applied in a homogeneous, or mix-and-measure, assay format.
G J R, Zaman, A, Garritsen, Th, de Boer, C A A, van Boeckel   +3 more
openaire   +2 more sources