Results 81 to 90 of about 1,074,205 (395)

Cell wall target fragment discovery using a low‐cost, minimal fragment library

FEBS Letters, EarlyView.
LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan, Mathew Stanley, Olawale Raimi, Andrew T. Ferenbach, Helge C Dorfmueller, Daan M. F. van Aalten   +5 more
wiley   +1 more source

Transkingdom Networks: A Systems Biology Approach to Identify Causal Members of Host-Microbiota Interactions

, 2017
Improvements in sequencing technologies and reduced experimental costs have resulted in a vast number of studies generating high-throughput data. Although the number of methods to analyze these "omics" data has also increased, computational complexity ...
A Fuente de la, A Morgun, A Morgun, A Mortazavi, A Perez-Diez, A Yambartsev, AR Erickson, AR Joyce, B Buchfink, B Langmead, BJ Haas, C Trapnell, CL Poirel, CL Schoch, DH Huson, DJ Lane, DJ McCarthy, E Saccenti, E Stackebrandt, ER Mardis, F Imhann, F Meyer, Gautier, J Kuczynski, J Skinner, JG Caporaso, JL Brookman, JN Paulson, KL Mine, LD Thomas, M Martin, M Schirmer, MA Moreno-Risueno, ML Metzker, N Gehlenborg, N Krumm, N Segata, N Shulzhenko, O Morozova, P Li, PD Schloss, PW Laird, R Greer, R Schmieder, Ritchie, RL Greer, RR Rodrigues, S Anders, S Goodwin, S Lindgreen, S Weiss, S Zhao, SC Schuster, TJ Sharpton, W Zhang, X Dong, YJ Hua   +56 more
core   +1 more source

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

Species Identification and Profiling of Complex Microbial Communities Using Shotgun Illumina Sequencing of 16S rRNA Amplicon Sequences [PDF]

, 2013
The high throughput and cost-effectiveness afforded by short-read sequencing technologies, in principle, enable researchers to perform 16S rRNA profiling of complex microbial communities at unprecedented depth and resolution. Existing Illumina sequencing
Hibberd, Martin Lloyd, Ho, Eliza Xin Pei, Kukkillaya, Vinutha Uppoor, Lay, Christophe, Low, Louie, Nagarajan, Niranjan, Ong, Swee Hoe, Wilm, Andreas   +7 more
core   +4 more sources

Characterizing the salivary RNA landscape to identify potential diagnostic, prognostic, and follow‐up biomarkers for breast cancer

Molecular Oncology, EarlyView.
This study explores salivary RNA for breast cancer (BC) diagnosis, prognosis, and follow‐up. High‐throughput RNA sequencing identified distinct salivary RNA signatures, including novel transcripts, that differentiate BC from healthy controls, characterize histological and molecular subtypes, and indicate lymph node involvement.
Nicholas Rajan, Irina Primac, Emre Etlioglu, Laurens Debruyne, Ann Janssen, Magy Sallam, Kevin Tabury, Roel Quintens, Wiebren Tjalma, Mohammed Abderrafi Benotmane   +9 more
wiley   +1 more source

Variants of uncertain significance in the era of high-throughput genome sequencing: a lesson from breast and ovary cancers

Journal of experimental & clinical cancer research : CR, 2020
The promising expectations about personalized medicine have opened the path to routine large-scale sequencing and increased the importance of genetic counseling for hereditary cancers, among which hereditary breast and ovary cancers (HBOC) have a major ...
G. Federici, S. Soddu
semanticscholar   +1 more source

High throughput genetic sequence analysis [PDF]

Proceedings of the 11th ACM Conference on Computing Frontiers, 2014
We present an application paradigm in which an unsupervised machine learning approach is applied to high dimensional influenza sequence datasets: (1) human A/H3N2, (2) avian H5, and (3) North American swine influenza H3N2 virus. Interesting visual patterns observed in the A/H3N2 influenza virus led us to hypothesize that vaccination could be one of the
Ham Ching Lam, Steve Cunningham, Srinand Sreevatsan, Daniel Boley   +3 more
openaire   +1 more source

Bridging the gap: Multi‐stakeholder perspectives of molecular diagnostics in oncology

Molecular Oncology, EarlyView.
Although molecular diagnostics is transforming cancer care, implementing novel technologies remains challenging. This study identifies unmet needs and technology requirements through a two‐step stakeholder involvement. Liquid biopsies for monitoring applications and predictive biomarker testing emerge as key unmet needs. Technology requirements vary by
Jorine Arnouts, Senada Koljenović, Elise Daems, Karolien De Wael, Marc Peeters, Léon C. van Kempen, Greetje Vanhoutte, Karen Zwaenepoel, Timon Vandamme   +8 more
wiley   +1 more source

High Throughput Sequencing For Plant Virus Detection and Discovery.

Phytopathology, 2019
Over the last decade, virologists have discovered an unprecedented number of viruses using high throughput sequencing (HTS), which led to the advancement of our knowledge on the diversity of viruses in nature, particularly unraveling the virome of many ...
D. Villamor, T. Hồ, M. Rwahnih, Robert R. Martin, I. Tzanetakis   +4 more
semanticscholar   +1 more source

Predictors of response and rational combinations for the novel MCL‐1 inhibitor MIK665 in acute myeloid leukemia

Molecular Oncology, EarlyView.
This study characterizes the responses of primary acute myeloid leukemia (AML) patient samples to the MCL‐1 inhibitor MIK665. The results revealed that monocytic differentiation is associated with MIK665 sensitivity. Conversely, elevated ABCB1 expression is a potential biomarker of resistance to the treatment, which can be overcome by the combination ...
Joseph Saad, Rhiannon Newman, Elmira Khabusheva, Sofia Aakko, Eric Durand, Mahesh Tambe, Heikki Kuusanmäki, Alun Parsons, Juho J. Miettinen, Komal Kumar Javarappa, Ezgi June Olgac, Nemo Ikonen, Mika Kontro, Kimmo Porkka, Heiko Maacke, Janghee Woo, Ensar Halilovic, Caroline A. Heckman   +17 more
wiley   +1 more source