Results 61 to 70 of about 902,297 (302)

Investigation of gut microbiome changes in type 1 diabetic mellitus rats based on high-throughput sequencing.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2020
The incidence of type 1 diabetes mellitus (T1DM) is increasing year by year, gut microbiota is considered to be closely related to the occurrence and development of T1DM in recent years.
Quantao Ma, Yaqi Li, Jingkang Wang, Pengfei Li, Yuhui Duan, Hongyu Dai, Yongcheng An, Long Cheng, Tie-shan Wang, Chunguo Wang, Ting-Ye Wang, Baosheng Zhao   +11 more
semanticscholar   +1 more source

Transcriptome-Wide High-Throughput m6A Sequencing of Differential m6A Methylation Patterns in the Human Rheumatoid Arthritis Fibroblast-Like Synoviocytes Cell Line MH7A

Journal of Inflammation Research, 2021
Hui Jiang1,2 *,* Kefeng Cao3 *,* Chang Fan,1,2 Xiaoya Cui,1,2 Yanzhen Ma,1,2 Jian Liu1 1Experimental Center of Clinical Research, First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, Anhui, People’s Republic of China; 2School ...
Jiang H, Cao K, Fan C, Cui X, Ma Y, Liu J   +5 more
doaj  

Phigaro: high-throughput prophage sequence annotation [PDF]

Bioinformatics, 2020
Abstract Summary Phigaro is a standalone command-line application that is able to detect prophage regions taking raw genome and metagenome assemblies as an input. It also produces dynamic annotated ‘prophage genome maps’ and marks possible transposon insertion spots inside prophages.
Elizaveta V. Starikova, Polina O. Tikhonova, Nikita A. Prianichnikov, Chris M. Rands, Evgeny M. Zdobnov, Elena N. Ilina, Vadim M. Govorun   +6 more
openaire   +2 more sources

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

Robust Sub-nanomolar Library Preparation for High Throughput Next Generation Sequencing

BMC Genomics, 2018
Background Current library preparation protocols for Illumina HiSeq and MiSeq DNA sequencers require ≥2 nM initial library for subsequent loading of denatured cDNA onto flow cells.
Wells W. Wu, Je-Nie Phue, Chun-Ting Lee, Changyi Lin, Lai Xu, Rong Wang, Yaqin Zhang, Rong-Fong Shen   +7 more
doaj   +1 more source

Breaking Barriers with Bread: Using the Sourdough Starter Microbiome to Teach High-Throughput Sequencing Techniques

Journal of Microbiology & Biology Education, 2022
Widespread usage of high-throughput sequencing (HTS) in the LIFE SCIENCES has produced a demand for undergraduate and graduate institutions to offer classes exposing students to all aspects of HTS (sample acquisition, laboratory work, sequencing ...
Benjamin H. Holt, Alison Buchan, Jennifer M. DeBruyn, Heidi Goodrich-Blair, Elizabeth McPherson, Veronica A. Brown   +5 more
doaj   +1 more source

High-Throughput Sequencing Survey of Sunflower Soil

Microbiology Resource Announcements, 2021
Plant rhizobiomes are responsible for major soil processes in the immediate plant environment, but our knowledge of the linkage between below-ground microbiota diversity and plant health is limited. We studied the bacterial and archaeal communities of sunflower rhizosphere organisms by comparing the composition of these communities in bulk soil at ...
Olubukola Oluranti Babalola, Blessing Chidinma Nwachukwu, Ayansina Segun Ayangbenro   +2 more
openaire   +2 more sources

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source

Performance comparison of benchtop high-throughput sequencing platforms

Nature Biotechnology, 2012
Three benchtop high-throughput sequencing instruments are now available. The 454 GS Junior (Roche), MiSeq (Illumina) and Ion Torrent PGM (Life Technologies) are laser-printer sized and offer modest set-up and running costs.
N. Loman, Raju V. Misra, T. Dallman, C. Constantinidou, S. Gharbia, J. Wain, M. Pallen   +6 more
semanticscholar   +1 more source

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source