Results 51 to 60 of about 902,297 (302)

HaploGrep 2: mitochondrial haplogroup classification in the era of high-throughput sequencing

Nucleic Acids Res., 2016
Mitochondrial DNA (mtDNA) profiles can be classified into phylogenetic clusters (haplogroups), which is of great relevance for evolutionary, forensic and medical genetics.
H. Weissensteiner, D. Pacher, A. Kloss-Brandstätter, L. Forer, Günther Specht, H. Bandelt, F. Kronenberg, A. Salas, S. Schönherr   +8 more
semanticscholar   +1 more source

High-throughput sequencing of a South American Amerindian. [PDF]

PLoS ONE, 2013
The emergence of next-generation sequencing technologies allowed access to the vast amounts of information that are contained in the human genome. This information has contributed to the understanding of individual and population-based variability and ...
André M Ribeiro-dos-Santos, Jorge Estefano Santana de Souza, Renan Almeida, Dayse O Alencar, Maria Silvanira Barbosa, Leonor Gusmão, Wilson A Silva, Sandro J de Souza, Artur Silva, Ândrea Ribeiro-dos-Santos, Sylvain Darnet, Sidney Santos   +11 more
doaj   +1 more source

Unifying the analysis of high-throughput sequencing datasets: characterizing RNA-seq, 16S rRNA gene sequencing and selective growth experiments by compositional data analysis

Microbiome, 2014
Experimental designs that take advantage of high-throughput sequencing to generate datasets include RNA sequencing (RNA-seq), chromatin immunoprecipitation sequencing (ChIP-seq), sequencing of 16S rRNA gene fragments, metagenomic analysis and selective ...
A. Fernandes, J. N. Reid, Jean M. Macklaim, T. A. McMurrough, D. Edgell, G. Gloor   +5 more
semanticscholar   +1 more source

ReSeq simulates realistic Illumina high-throughput sequencing data

Genome Biology, 2021
In high-throughput sequencing data, performance comparisons between computational tools are essential for making informed decisions at each step of a project.
Stephan Schmeing, Mark D. Robinson
doaj   +1 more source

LoFreq: a sequence-quality aware, ultra-sensitive variant caller for uncovering cell-population heterogeneity from high-throughput sequencing datasets

Nucleic Acids Research, 2012
The study of cell-population heterogeneity in a range of biological systems, from viruses to bacterial isolates to tumor samples, has been transformed by recent advances in sequencing throughput. While the high-coverage afforded can be used, in principle,
A. Wilm, P. Aw, D. Bertrand, G. Yeo, S. Ong, C. Wong, C. Khor, R. Petric, M. Hibberd, N. Nagarajan   +9 more
semanticscholar   +1 more source

Efficient COI barcoding using high throughput single-end 400 bp sequencing

BMC Genomics, 2020
Background Over the last decade, the rapid development of high-throughput sequencing platforms has accelerated species description and assisted morphological classification through DNA barcoding. However, the current high-throughput DNA barcoding methods
Chentao Yang, Yuxuan Zheng, Shangjin Tan, Guanliang Meng, Wei Rao, Caiqing Yang, David G. Bourne, Paul A. O’Brien, Junqiang Xu, Sha Liao, Ao Chen, Xiaowei Chen, Xinrui Jia, Ai-bing Zhang, Shanlin Liu   +14 more
doaj   +1 more source

Scrutinizing virus genome termini by high-throughput sequencing. [PDF]

PLoS ONE, 2014
Analysis of genomic terminal sequences has been a major step in studies on viral DNA replication and packaging mechanisms. However, traditional methods to study genome termini are challenging due to the time-consuming protocols and their inefficiency ...
Shasha Li, Hang Fan, Xiaoping An, Huahao Fan, Huanhuan Jiang, Yubao Chen, Yigang Tong   +6 more
doaj   +1 more source

SnapShot: High-Throughput Sequencing Applications

Cell, 2011
/ 25 (X2) 8 daysPacific Biosciences http://www.pacificbiosciences.comNo amplification; Synthesis Fluorescence >1000 bp (SE) 150,000 per SMRT cell N/AIon Torrent http://www.iontorrent.comEmulsion PCR; Synthesis Change in pH 200 bp (SE) Variable 90% of the full genome resolved (both alleles)400 human genomes per monthModified and updated from Metzker ...
Han, Hong, Nutiu, Razvan, Moffat, Jason, Blencowe, Benjamin J.   +3 more
openaire   +2 more sources

High-throughput sequencing of the transcriptome and chromatin accessibility in the same cell

Nature Biotechnology, 2019
Single-cell RNA sequencing can reveal the transcriptional state of cells, yet provides little insight into the upstream regulatory landscape associated with open or accessible chromatin regions.
S. Chen, B. Lake, Kun Zhang
semanticscholar   +1 more source

High-throughput sequencing: a failure mode analysis

BMC Genomics, 2005
Background Basic manufacturing principles are becoming increasingly important in high-throughput sequencing facilities where there is a constant drive to increase quality, increase efficiency, and decrease operating costs.
Marra Marco A, Balasundaram Miruna, Barber Sarah A, Smailus Duane, Stott Jeffery M, Yang George S, Holt Robert A   +6 more
doaj   +1 more source